Dietary black soldier fly oil enhances growth performance, flesh quality, and health status of largemouth bass (Micropterus salmoides)

The study aimed to assess the effects of dietary black soldier fly oil (BSFO) on the growth performance, flesh quality, and health status of largemouth bass (Micropterus salmoides). Six iso-nitrogenous and isolipid diets were formulated by substituting fish oil and soybean oil (1/2, wt/wt) with BSFO...

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Main Authors: Hailin Yuan, Junru Hu, Xiangce Li, Qiuxuan Sun, Xiaohong Tan, Cuihong You, Yewei Dong, Yanhua Huang, Meng Zhou
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2024-09-01
Series:Animal Nutrition
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405654524000702
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author Hailin Yuan
Junru Hu
Xiangce Li
Qiuxuan Sun
Xiaohong Tan
Cuihong You
Yewei Dong
Yanhua Huang
Meng Zhou
author_facet Hailin Yuan
Junru Hu
Xiangce Li
Qiuxuan Sun
Xiaohong Tan
Cuihong You
Yewei Dong
Yanhua Huang
Meng Zhou
author_sort Hailin Yuan
collection DOAJ
description The study aimed to assess the effects of dietary black soldier fly oil (BSFO) on the growth performance, flesh quality, and health status of largemouth bass (Micropterus salmoides). Six iso-nitrogenous and isolipid diets were formulated by substituting fish oil and soybean oil (1/2, wt/wt) with BSFO in percentages of 0%, 20%, 40%, 60%, 80%, and 100%, respectively. The diets were fed to 960 fish (initial body weight = 16.5 g) in four replicates for 8 weeks. Indicators related to growth performance, body composition, hematology, flesh quality, expression of genes related to inflammatory cytokines and apoptosis, and the response of fish to Aeromonas veronii challenge were analyzed. The results showed that the weight gain rate was numerically improved in all BSFO substitution groups, ranging from 9.3% to 44.0% compared to the control group. The highest survival rate and the lowest hepatosomatic index and condition factor were observed in the BFSO20 group. In terms of flesh quality, the water-holding capacity of the dorsal muscle was elevated with higher levels of dietary BSFO. However, significant changes in texture properties (cohesiveness, gluing, and chewiness) were observed in the BSFO20 group (P < 0.05). Six hematological parameters related to glycolipid and liver function were optimized in most of the BFSO substitution groups. Furthermore, the expressions of six inflammation- and apoptosis-related genes (IL-1β, Bcl-xl, BAX, caspase8, TNF-α, and IL-10) were significantly affected by dietary BSFO (P < 0.05). Following bacterial challenge, the seven-day cumulative survival rates of fish were considerably increased from 10.0% in the control group to 60.0% and 66.7% in the BSFO80 and BSFO100 groups, respectively. One-variable linear regression analysis revealed that various parameters related to fish growth, flesh quality, and health status were significantly influenced by dietary BSFO substitution levels in a dose-dependent manner (P < 0.05). In conclusion, substituting around 20% of dietary fish oil and soybean oil with BSFO is promising in improving the growth performance and flesh quality of M. salmoides. However, to enhance immunity and disease resistance, it is recommended to further increase the inclusion of BSFO in the diet.
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spelling doaj-art-c9cda1a7e98e4bb0876ccd53038a73f82025-08-20T02:09:52ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452024-09-011823424510.1016/j.aninu.2024.03.019Dietary black soldier fly oil enhances growth performance, flesh quality, and health status of largemouth bass (Micropterus salmoides)Hailin Yuan0Junru Hu1Xiangce Li2Qiuxuan Sun3Xiaohong Tan4Cuihong You5Yewei Dong6Yanhua Huang7Meng Zhou8Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China; Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangzhou 510225, Guangdong, China; Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou 510225, Guangdong, ChinaGuangdong Provincial Key Laboratory of Animal Breeding and Nutrition Research, Guangzhou 510640, Guangdong, ChinaInnovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China; Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangzhou 510225, Guangdong, China; Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou 510225, Guangdong, ChinaInnovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China; Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangzhou 510225, Guangdong, China; Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou 510225, Guangdong, ChinaInnovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China; Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangzhou 510225, Guangdong, China; Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou 510225, Guangdong, ChinaInnovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China; Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangzhou 510225, Guangdong, China; Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou 510225, Guangdong, ChinaInnovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China; Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangzhou 510225, Guangdong, China; Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou 510225, Guangdong, ChinaInnovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China; Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangzhou 510225, Guangdong, China; Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou 510225, Guangdong, China; Corresponding authors.Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China; Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangzhou 510225, Guangdong, China; Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou 510225, Guangdong, China; Corresponding authors.The study aimed to assess the effects of dietary black soldier fly oil (BSFO) on the growth performance, flesh quality, and health status of largemouth bass (Micropterus salmoides). Six iso-nitrogenous and isolipid diets were formulated by substituting fish oil and soybean oil (1/2, wt/wt) with BSFO in percentages of 0%, 20%, 40%, 60%, 80%, and 100%, respectively. The diets were fed to 960 fish (initial body weight = 16.5 g) in four replicates for 8 weeks. Indicators related to growth performance, body composition, hematology, flesh quality, expression of genes related to inflammatory cytokines and apoptosis, and the response of fish to Aeromonas veronii challenge were analyzed. The results showed that the weight gain rate was numerically improved in all BSFO substitution groups, ranging from 9.3% to 44.0% compared to the control group. The highest survival rate and the lowest hepatosomatic index and condition factor were observed in the BFSO20 group. In terms of flesh quality, the water-holding capacity of the dorsal muscle was elevated with higher levels of dietary BSFO. However, significant changes in texture properties (cohesiveness, gluing, and chewiness) were observed in the BSFO20 group (P < 0.05). Six hematological parameters related to glycolipid and liver function were optimized in most of the BFSO substitution groups. Furthermore, the expressions of six inflammation- and apoptosis-related genes (IL-1β, Bcl-xl, BAX, caspase8, TNF-α, and IL-10) were significantly affected by dietary BSFO (P < 0.05). Following bacterial challenge, the seven-day cumulative survival rates of fish were considerably increased from 10.0% in the control group to 60.0% and 66.7% in the BSFO80 and BSFO100 groups, respectively. One-variable linear regression analysis revealed that various parameters related to fish growth, flesh quality, and health status were significantly influenced by dietary BSFO substitution levels in a dose-dependent manner (P < 0.05). In conclusion, substituting around 20% of dietary fish oil and soybean oil with BSFO is promising in improving the growth performance and flesh quality of M. salmoides. However, to enhance immunity and disease resistance, it is recommended to further increase the inclusion of BSFO in the diet.http://www.sciencedirect.com/science/article/pii/S2405654524000702Micropterus salmoidesBlack soldier fly oil (BSFO)Growth performanceFlesh qualityHealth status
spellingShingle Hailin Yuan
Junru Hu
Xiangce Li
Qiuxuan Sun
Xiaohong Tan
Cuihong You
Yewei Dong
Yanhua Huang
Meng Zhou
Dietary black soldier fly oil enhances growth performance, flesh quality, and health status of largemouth bass (Micropterus salmoides)
Animal Nutrition
Micropterus salmoides
Black soldier fly oil (BSFO)
Growth performance
Flesh quality
Health status
title Dietary black soldier fly oil enhances growth performance, flesh quality, and health status of largemouth bass (Micropterus salmoides)
title_full Dietary black soldier fly oil enhances growth performance, flesh quality, and health status of largemouth bass (Micropterus salmoides)
title_fullStr Dietary black soldier fly oil enhances growth performance, flesh quality, and health status of largemouth bass (Micropterus salmoides)
title_full_unstemmed Dietary black soldier fly oil enhances growth performance, flesh quality, and health status of largemouth bass (Micropterus salmoides)
title_short Dietary black soldier fly oil enhances growth performance, flesh quality, and health status of largemouth bass (Micropterus salmoides)
title_sort dietary black soldier fly oil enhances growth performance flesh quality and health status of largemouth bass micropterus salmoides
topic Micropterus salmoides
Black soldier fly oil (BSFO)
Growth performance
Flesh quality
Health status
url http://www.sciencedirect.com/science/article/pii/S2405654524000702
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