Comprehensive characterization of the competitive endogenous RNA network revealing its immune-related functions in hepatic ischemia-reperfusion injury.
Hepatic ischemia-reperfusion injury (HIRI) is a common complication in liver surgery and transplantation. Recent studies have revealed the significant role of the competing endogenous RNA (ceRNA) network in HIRI. Herein, we comprehensively analyzed the HIRI-related ceRNA network and its correlation...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0327101 |
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| author | Lirong Liu Qi Wang Shuang Qin Chang Su Xin Hai Zhenkuan Yuan |
| author_facet | Lirong Liu Qi Wang Shuang Qin Chang Su Xin Hai Zhenkuan Yuan |
| author_sort | Lirong Liu |
| collection | DOAJ |
| description | Hepatic ischemia-reperfusion injury (HIRI) is a common complication in liver surgery and transplantation. Recent studies have revealed the significant role of the competing endogenous RNA (ceRNA) network in HIRI. Herein, we comprehensively analyzed the HIRI-related ceRNA network and its correlation with immune-related pathways and immune cells in HIRI patients. We identified 449 lncRNAs, 26 miRNAs, and 548 mRNAs differentially expressed in HIRI patients. We constructed a HIRI-related ceRNA network in liver transplant patients consisting of 3 lncRNAs, 3 miRNAs, and 29 mRNAs. Biological function analysis showed that the HIRI-related ceRNA network contributes to HIRI progression by regulating calcium ion-related regulatory pathways and processes. An immune-related ceRNA subnetwork, which consists of 1 lncRNA (PARD6G-AS1), 1 miRNA (hsa-miRNA-125b-5p), and 4 mRNAs (PLAU, CCR5, FGF5 and IL24) was obtained. The immune-related ceRNA subnetwork was significantly related to the immune-related pathways and immune cell infiltration. The PARD6G-AS1/miR-125b-5p/IL24 axis was identified as a potential ceRNA sponge that may influence NK cell activity in HIRI. Our results underlined that the lncRNA-miRNA-mRNA ceRNA network can positively or negatively regulate immune-related functions and infiltrating immune cells mediated HIRI, which could provide further insight into novel molecular therapeutic targets. |
| format | Article |
| id | doaj-art-c9bd55fb5ea84ea590a97c8ee8f2c122 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-c9bd55fb5ea84ea590a97c8ee8f2c1222025-08-20T03:42:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01207e032710110.1371/journal.pone.0327101Comprehensive characterization of the competitive endogenous RNA network revealing its immune-related functions in hepatic ischemia-reperfusion injury.Lirong LiuQi WangShuang QinChang SuXin HaiZhenkuan YuanHepatic ischemia-reperfusion injury (HIRI) is a common complication in liver surgery and transplantation. Recent studies have revealed the significant role of the competing endogenous RNA (ceRNA) network in HIRI. Herein, we comprehensively analyzed the HIRI-related ceRNA network and its correlation with immune-related pathways and immune cells in HIRI patients. We identified 449 lncRNAs, 26 miRNAs, and 548 mRNAs differentially expressed in HIRI patients. We constructed a HIRI-related ceRNA network in liver transplant patients consisting of 3 lncRNAs, 3 miRNAs, and 29 mRNAs. Biological function analysis showed that the HIRI-related ceRNA network contributes to HIRI progression by regulating calcium ion-related regulatory pathways and processes. An immune-related ceRNA subnetwork, which consists of 1 lncRNA (PARD6G-AS1), 1 miRNA (hsa-miRNA-125b-5p), and 4 mRNAs (PLAU, CCR5, FGF5 and IL24) was obtained. The immune-related ceRNA subnetwork was significantly related to the immune-related pathways and immune cell infiltration. The PARD6G-AS1/miR-125b-5p/IL24 axis was identified as a potential ceRNA sponge that may influence NK cell activity in HIRI. Our results underlined that the lncRNA-miRNA-mRNA ceRNA network can positively or negatively regulate immune-related functions and infiltrating immune cells mediated HIRI, which could provide further insight into novel molecular therapeutic targets.https://doi.org/10.1371/journal.pone.0327101 |
| spellingShingle | Lirong Liu Qi Wang Shuang Qin Chang Su Xin Hai Zhenkuan Yuan Comprehensive characterization of the competitive endogenous RNA network revealing its immune-related functions in hepatic ischemia-reperfusion injury. PLoS ONE |
| title | Comprehensive characterization of the competitive endogenous RNA network revealing its immune-related functions in hepatic ischemia-reperfusion injury. |
| title_full | Comprehensive characterization of the competitive endogenous RNA network revealing its immune-related functions in hepatic ischemia-reperfusion injury. |
| title_fullStr | Comprehensive characterization of the competitive endogenous RNA network revealing its immune-related functions in hepatic ischemia-reperfusion injury. |
| title_full_unstemmed | Comprehensive characterization of the competitive endogenous RNA network revealing its immune-related functions in hepatic ischemia-reperfusion injury. |
| title_short | Comprehensive characterization of the competitive endogenous RNA network revealing its immune-related functions in hepatic ischemia-reperfusion injury. |
| title_sort | comprehensive characterization of the competitive endogenous rna network revealing its immune related functions in hepatic ischemia reperfusion injury |
| url | https://doi.org/10.1371/journal.pone.0327101 |
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