Population structure of Hispanics in the United States: the multi-ethnic study of atherosclerosis.

Using ~60,000 SNPs selected for minimal linkage disequilibrium, we perform population structure analysis of 1,374 unrelated Hispanic individuals from the Multi-Ethnic Study of Atherosclerosis (MESA), with self-identification corresponding to Central America (n = 93), Cuba (n = 50), the Dominican Rep...

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Main Authors: Ani Manichaikul, Walter Palmas, Carlos J Rodriguez, Carmen A Peralta, Jasmin Divers, Xiuqing Guo, Wei-Min Chen, Quenna Wong, Kayleen Williams, Kathleen F Kerr, Kent D Taylor, Michael Y Tsai, Mark O Goodarzi, Michèle M Sale, Ana V Diez-Roux, Stephen S Rich, Jerome I Rotter, Josyf C Mychaleckyj
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS Genetics
Online Access:https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1002640&type=printable
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author Ani Manichaikul
Walter Palmas
Carlos J Rodriguez
Carmen A Peralta
Jasmin Divers
Xiuqing Guo
Wei-Min Chen
Quenna Wong
Kayleen Williams
Kathleen F Kerr
Kent D Taylor
Michael Y Tsai
Mark O Goodarzi
Michèle M Sale
Ana V Diez-Roux
Stephen S Rich
Jerome I Rotter
Josyf C Mychaleckyj
author_facet Ani Manichaikul
Walter Palmas
Carlos J Rodriguez
Carmen A Peralta
Jasmin Divers
Xiuqing Guo
Wei-Min Chen
Quenna Wong
Kayleen Williams
Kathleen F Kerr
Kent D Taylor
Michael Y Tsai
Mark O Goodarzi
Michèle M Sale
Ana V Diez-Roux
Stephen S Rich
Jerome I Rotter
Josyf C Mychaleckyj
author_sort Ani Manichaikul
collection DOAJ
description Using ~60,000 SNPs selected for minimal linkage disequilibrium, we perform population structure analysis of 1,374 unrelated Hispanic individuals from the Multi-Ethnic Study of Atherosclerosis (MESA), with self-identification corresponding to Central America (n = 93), Cuba (n = 50), the Dominican Republic (n = 203), Mexico (n = 708), Puerto Rico (n = 192), and South America (n = 111). By projection of principal components (PCs) of ancestry to samples from the HapMap phase III and the Human Genome Diversity Panel (HGDP), we show the first two PCs quantify the Caucasian, African, and Native American origins, while the third and fourth PCs bring out an axis that aligns with known South-to-North geographic location of HGDP Native American samples and further separates MESA Mexican versus Central/South American samples along the same axis. Using k-means clustering computed from the first four PCs, we define four subgroups of the MESA Hispanic cohort that show close agreement with self-identification, labeling the clusters as primarily Dominican/Cuban, Mexican, Central/South American, and Puerto Rican. To demonstrate our recommendations for genetic analysis in the MESA Hispanic cohort, we present pooled and stratified association analysis of triglycerides for selected SNPs in the LPL and TRIB1 gene regions, previously reported in GWAS of triglycerides in Caucasians but as yet unconfirmed in Hispanic populations. We report statistically significant evidence for genetic association in both genes, and we further demonstrate the importance of considering population substructure and genetic heterogeneity in genetic association studies performed in the United States Hispanic population.
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spelling doaj-art-c9afd64b91424191b6d2b5c2f087e31c2025-08-20T03:10:25ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042012-01-0184e100264010.1371/journal.pgen.1002640Population structure of Hispanics in the United States: the multi-ethnic study of atherosclerosis.Ani ManichaikulWalter PalmasCarlos J RodriguezCarmen A PeraltaJasmin DiversXiuqing GuoWei-Min ChenQuenna WongKayleen WilliamsKathleen F KerrKent D TaylorMichael Y TsaiMark O GoodarziMichèle M SaleAna V Diez-RouxStephen S RichJerome I RotterJosyf C MychaleckyjUsing ~60,000 SNPs selected for minimal linkage disequilibrium, we perform population structure analysis of 1,374 unrelated Hispanic individuals from the Multi-Ethnic Study of Atherosclerosis (MESA), with self-identification corresponding to Central America (n = 93), Cuba (n = 50), the Dominican Republic (n = 203), Mexico (n = 708), Puerto Rico (n = 192), and South America (n = 111). By projection of principal components (PCs) of ancestry to samples from the HapMap phase III and the Human Genome Diversity Panel (HGDP), we show the first two PCs quantify the Caucasian, African, and Native American origins, while the third and fourth PCs bring out an axis that aligns with known South-to-North geographic location of HGDP Native American samples and further separates MESA Mexican versus Central/South American samples along the same axis. Using k-means clustering computed from the first four PCs, we define four subgroups of the MESA Hispanic cohort that show close agreement with self-identification, labeling the clusters as primarily Dominican/Cuban, Mexican, Central/South American, and Puerto Rican. To demonstrate our recommendations for genetic analysis in the MESA Hispanic cohort, we present pooled and stratified association analysis of triglycerides for selected SNPs in the LPL and TRIB1 gene regions, previously reported in GWAS of triglycerides in Caucasians but as yet unconfirmed in Hispanic populations. We report statistically significant evidence for genetic association in both genes, and we further demonstrate the importance of considering population substructure and genetic heterogeneity in genetic association studies performed in the United States Hispanic population.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1002640&type=printable
spellingShingle Ani Manichaikul
Walter Palmas
Carlos J Rodriguez
Carmen A Peralta
Jasmin Divers
Xiuqing Guo
Wei-Min Chen
Quenna Wong
Kayleen Williams
Kathleen F Kerr
Kent D Taylor
Michael Y Tsai
Mark O Goodarzi
Michèle M Sale
Ana V Diez-Roux
Stephen S Rich
Jerome I Rotter
Josyf C Mychaleckyj
Population structure of Hispanics in the United States: the multi-ethnic study of atherosclerosis.
PLoS Genetics
title Population structure of Hispanics in the United States: the multi-ethnic study of atherosclerosis.
title_full Population structure of Hispanics in the United States: the multi-ethnic study of atherosclerosis.
title_fullStr Population structure of Hispanics in the United States: the multi-ethnic study of atherosclerosis.
title_full_unstemmed Population structure of Hispanics in the United States: the multi-ethnic study of atherosclerosis.
title_short Population structure of Hispanics in the United States: the multi-ethnic study of atherosclerosis.
title_sort population structure of hispanics in the united states the multi ethnic study of atherosclerosis
url https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1002640&type=printable
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