Quantification of Myoinositol in Serum by Electrochemical Detection with an Unmodified Screen-Printed Carbon Electrode
Simple, rapid, and accurate detection of myoinositol (MI) concentration in blood is crucial in diagnosing polycystic ovary syndrome, neurological disorders, and cancer. A novel electrochemical detection (IED) method was established to quantify MI in human serum using a disposable unmodified screen-p...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/3998338 |
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| author | Xinrui Jin Yuanqing Zhao Xiujuan Gu Min Zhong Xin Kong Guangrong Li Gang Tian Jinbo Liu |
| author_facet | Xinrui Jin Yuanqing Zhao Xiujuan Gu Min Zhong Xin Kong Guangrong Li Gang Tian Jinbo Liu |
| author_sort | Xinrui Jin |
| collection | DOAJ |
| description | Simple, rapid, and accurate detection of myoinositol (MI) concentration in blood is crucial in diagnosing polycystic ovary syndrome, neurological disorders, and cancer. A novel electrochemical detection (IED) method was established to quantify MI in human serum using a disposable unmodified screen-printed carbon electrode (SPCE) for the first time. MI was detected indirectly by the reaction product of myoinositol dehydrogenase (IDH) and cofactor β-nicotinamide adenine dinucleotide (NAD+). Good linear calibration curves were obtained at the concentration range from 5.0 μM to 500.0 μM (R2 = 0.9981) with the lower limits of detection (LOD) and quantification (LOQ) of 1.0 μM and 2.5 μM, respectively. Recoveries were calculated at three spiked concentrations, and the values were between 90.3 and 106%, with relative standard deviation values of 3.2–6.2% for intraday precision and 7.1–9.0% for interday precision. The SPCE-electrochemical biosensor is simple, accurate, and without modification, showing great potential for point-of-care testing (POCT) of serum MI in clinical samples. |
| format | Article |
| id | doaj-art-c9ae83c773104f938f90aeb20f031de3 |
| institution | DOAJ |
| issn | 2090-8873 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-c9ae83c773104f938f90aeb20f031de32025-08-20T03:23:59ZengWileyJournal of Analytical Methods in Chemistry2090-88732022-01-01202210.1155/2022/3998338Quantification of Myoinositol in Serum by Electrochemical Detection with an Unmodified Screen-Printed Carbon ElectrodeXinrui Jin0Yuanqing Zhao1Xiujuan Gu2Min Zhong3Xin Kong4Guangrong Li5Gang Tian6Jinbo Liu7Department of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineSimple, rapid, and accurate detection of myoinositol (MI) concentration in blood is crucial in diagnosing polycystic ovary syndrome, neurological disorders, and cancer. A novel electrochemical detection (IED) method was established to quantify MI in human serum using a disposable unmodified screen-printed carbon electrode (SPCE) for the first time. MI was detected indirectly by the reaction product of myoinositol dehydrogenase (IDH) and cofactor β-nicotinamide adenine dinucleotide (NAD+). Good linear calibration curves were obtained at the concentration range from 5.0 μM to 500.0 μM (R2 = 0.9981) with the lower limits of detection (LOD) and quantification (LOQ) of 1.0 μM and 2.5 μM, respectively. Recoveries were calculated at three spiked concentrations, and the values were between 90.3 and 106%, with relative standard deviation values of 3.2–6.2% for intraday precision and 7.1–9.0% for interday precision. The SPCE-electrochemical biosensor is simple, accurate, and without modification, showing great potential for point-of-care testing (POCT) of serum MI in clinical samples.http://dx.doi.org/10.1155/2022/3998338 |
| spellingShingle | Xinrui Jin Yuanqing Zhao Xiujuan Gu Min Zhong Xin Kong Guangrong Li Gang Tian Jinbo Liu Quantification of Myoinositol in Serum by Electrochemical Detection with an Unmodified Screen-Printed Carbon Electrode Journal of Analytical Methods in Chemistry |
| title | Quantification of Myoinositol in Serum by Electrochemical Detection with an Unmodified Screen-Printed Carbon Electrode |
| title_full | Quantification of Myoinositol in Serum by Electrochemical Detection with an Unmodified Screen-Printed Carbon Electrode |
| title_fullStr | Quantification of Myoinositol in Serum by Electrochemical Detection with an Unmodified Screen-Printed Carbon Electrode |
| title_full_unstemmed | Quantification of Myoinositol in Serum by Electrochemical Detection with an Unmodified Screen-Printed Carbon Electrode |
| title_short | Quantification of Myoinositol in Serum by Electrochemical Detection with an Unmodified Screen-Printed Carbon Electrode |
| title_sort | quantification of myoinositol in serum by electrochemical detection with an unmodified screen printed carbon electrode |
| url | http://dx.doi.org/10.1155/2022/3998338 |
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