Drug Target Identification and Prioritization for Treatment of Ovine Foot Rot: An In Silico Approach
Ovine foot rot is an infection of the feet of sheep, mainly caused by Dichelobacter nodosus. In its virulent form, it is highly contagious and debilitating, causing significant losses in the form of decline in wool growth and quality and poor fertility. Current methods of treatment are ineffective i...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2016/7361361 |
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| author | Abhishek Acharya Lalit C. Garg |
| author_facet | Abhishek Acharya Lalit C. Garg |
| author_sort | Abhishek Acharya |
| collection | DOAJ |
| description | Ovine foot rot is an infection of the feet of sheep, mainly caused by Dichelobacter nodosus. In its virulent form, it is highly contagious and debilitating, causing significant losses in the form of decline in wool growth and quality and poor fertility. Current methods of treatment are ineffective in complete eradication. Effective antibiotic treatment of foot rot is hence necessary to ensure better outcomes during control phases by reduction in culling count and the possibility of carriers of the infection. Using computational approaches, we have identified a set of 297 proteins that are essential to the D. nodosus and nonhomologous with sheep proteins. These proteins may be considered as potential vaccine candidates or drug targets for designing antibiotics against the bacterium. This core set of drug targets have been analyzed for pathway annotation to identify 67 proteins involved in unique bacterial pathways. Choke-point analysis on the drug targets identified 138 choke-point proteins, 29 involved in unique bacterial pathways. Subcellular localization was also predicted for each target to identify the ones that are membrane associated or secreted extracellularly. In addition, a total of 13 targets were identified that are common in at least 10 pathogenic bacterial species. |
| format | Article |
| id | doaj-art-c9aa7e5573344f62bd4828285ddded5d |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-c9aa7e5573344f62bd4828285ddded5d2025-02-03T01:27:17ZengWileyInternational Journal of Genomics2314-436X2314-43782016-01-01201610.1155/2016/73613617361361Drug Target Identification and Prioritization for Treatment of Ovine Foot Rot: An In Silico ApproachAbhishek Acharya0Lalit C. Garg1Gene Regulation Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, IndiaGene Regulation Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, IndiaOvine foot rot is an infection of the feet of sheep, mainly caused by Dichelobacter nodosus. In its virulent form, it is highly contagious and debilitating, causing significant losses in the form of decline in wool growth and quality and poor fertility. Current methods of treatment are ineffective in complete eradication. Effective antibiotic treatment of foot rot is hence necessary to ensure better outcomes during control phases by reduction in culling count and the possibility of carriers of the infection. Using computational approaches, we have identified a set of 297 proteins that are essential to the D. nodosus and nonhomologous with sheep proteins. These proteins may be considered as potential vaccine candidates or drug targets for designing antibiotics against the bacterium. This core set of drug targets have been analyzed for pathway annotation to identify 67 proteins involved in unique bacterial pathways. Choke-point analysis on the drug targets identified 138 choke-point proteins, 29 involved in unique bacterial pathways. Subcellular localization was also predicted for each target to identify the ones that are membrane associated or secreted extracellularly. In addition, a total of 13 targets were identified that are common in at least 10 pathogenic bacterial species.http://dx.doi.org/10.1155/2016/7361361 |
| spellingShingle | Abhishek Acharya Lalit C. Garg Drug Target Identification and Prioritization for Treatment of Ovine Foot Rot: An In Silico Approach International Journal of Genomics |
| title | Drug Target Identification and Prioritization for Treatment of Ovine Foot Rot: An In Silico Approach |
| title_full | Drug Target Identification and Prioritization for Treatment of Ovine Foot Rot: An In Silico Approach |
| title_fullStr | Drug Target Identification and Prioritization for Treatment of Ovine Foot Rot: An In Silico Approach |
| title_full_unstemmed | Drug Target Identification and Prioritization for Treatment of Ovine Foot Rot: An In Silico Approach |
| title_short | Drug Target Identification and Prioritization for Treatment of Ovine Foot Rot: An In Silico Approach |
| title_sort | drug target identification and prioritization for treatment of ovine foot rot an in silico approach |
| url | http://dx.doi.org/10.1155/2016/7361361 |
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