Effect of Benzyl Adenine Concentration on Callus Induction of Geranium Plants (Pelargonium graveolens L'Her) from Petiole and Leaf Explants
Geranium plant (Pelargonium graveolens L'Her) is one of the geranium oil producing plants that has many benefits. Callus culture is a technique that can be used to plant multiplication and increase production of secondary metabolites. This study aims to determine the effect of the concentration...
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University of Brawijaya
2020-02-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/376 |
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| author | Moch Faizul Huda Serafinah Indriyani Wahyu Widoretno |
| author_facet | Moch Faizul Huda Serafinah Indriyani Wahyu Widoretno |
| author_sort | Moch Faizul Huda |
| collection | DOAJ |
| description | Geranium plant (Pelargonium graveolens L'Her) is one of the geranium oil producing plants that has many benefits. Callus culture is a technique that can be used to plant multiplication and increase production of secondary metabolites. This study aims to determine the effect of the concentration of Benzyl Adenine on the formation of geranium callus from petiole and leaf explants. Callus induction was carried out by culturing petiole and leaf explants on MS medium + 0.1 mg.L-1 NAA + Benzyl Adenine (0; 0.5; 1; 1.5 and 2 mg.L-1). Callus morphological parameters, percentage of callus formation, and time of first callus formation were observed. The formation of geranium callus influenced by the explant type and the concentration of Benzyl Adenine. In the 2nd week, the geranium callus was initiated, light green colored with a compact callus texture. At 4th week, the percentage of callus formation containing NAA 0.1 mg.L-1 of petiole and leaf explants was 20% and 8%, whereas the percentage of callus formation on medium containing 0.1 mg.L-1 NAA combined with 0.5-2 mg.L-1 Benzyl Adenine of petiole and leaf explants was 52-80% and 24-52%. The best percentage of callus formation was found on the culture medium containing 1 mg.L-1 Benzyl Adenine, equaled 80% of petiole explants, and 52% of leaf explants. |
| format | Article |
| id | doaj-art-c99f6de217d04edaaf32e1a75669e550 |
| institution | DOAJ |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2020-02-01 |
| publisher | University of Brawijaya |
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| series | Journal of Experimental Life Science |
| spelling | doaj-art-c99f6de217d04edaaf32e1a75669e5502025-08-20T03:09:59ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552020-02-011012023https://doi.org/10.21776/ub.jels.2019.010.01.04Effect of Benzyl Adenine Concentration on Callus Induction of Geranium Plants (Pelargonium graveolens L'Her) from Petiole and Leaf ExplantsMoch Faizul Huda0Serafinah Indriyani1Wahyu Widoretno2Universitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaGeranium plant (Pelargonium graveolens L'Her) is one of the geranium oil producing plants that has many benefits. Callus culture is a technique that can be used to plant multiplication and increase production of secondary metabolites. This study aims to determine the effect of the concentration of Benzyl Adenine on the formation of geranium callus from petiole and leaf explants. Callus induction was carried out by culturing petiole and leaf explants on MS medium + 0.1 mg.L-1 NAA + Benzyl Adenine (0; 0.5; 1; 1.5 and 2 mg.L-1). Callus morphological parameters, percentage of callus formation, and time of first callus formation were observed. The formation of geranium callus influenced by the explant type and the concentration of Benzyl Adenine. In the 2nd week, the geranium callus was initiated, light green colored with a compact callus texture. At 4th week, the percentage of callus formation containing NAA 0.1 mg.L-1 of petiole and leaf explants was 20% and 8%, whereas the percentage of callus formation on medium containing 0.1 mg.L-1 NAA combined with 0.5-2 mg.L-1 Benzyl Adenine of petiole and leaf explants was 52-80% and 24-52%. The best percentage of callus formation was found on the culture medium containing 1 mg.L-1 Benzyl Adenine, equaled 80% of petiole explants, and 52% of leaf explants.https://jels.ub.ac.id/index.php/jels/article/view/376bacallusleafpetiolepelargonium graveolens l'her. |
| spellingShingle | Moch Faizul Huda Serafinah Indriyani Wahyu Widoretno Effect of Benzyl Adenine Concentration on Callus Induction of Geranium Plants (Pelargonium graveolens L'Her) from Petiole and Leaf Explants Journal of Experimental Life Science ba callus leaf petiole pelargonium graveolens l'her. |
| title | Effect of Benzyl Adenine Concentration on Callus Induction of Geranium Plants (Pelargonium graveolens L'Her) from Petiole and Leaf Explants |
| title_full | Effect of Benzyl Adenine Concentration on Callus Induction of Geranium Plants (Pelargonium graveolens L'Her) from Petiole and Leaf Explants |
| title_fullStr | Effect of Benzyl Adenine Concentration on Callus Induction of Geranium Plants (Pelargonium graveolens L'Her) from Petiole and Leaf Explants |
| title_full_unstemmed | Effect of Benzyl Adenine Concentration on Callus Induction of Geranium Plants (Pelargonium graveolens L'Her) from Petiole and Leaf Explants |
| title_short | Effect of Benzyl Adenine Concentration on Callus Induction of Geranium Plants (Pelargonium graveolens L'Her) from Petiole and Leaf Explants |
| title_sort | effect of benzyl adenine concentration on callus induction of geranium plants pelargonium graveolens l her from petiole and leaf explants |
| topic | ba callus leaf petiole pelargonium graveolens l'her. |
| url | https://jels.ub.ac.id/index.php/jels/article/view/376 |
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