Dietary regulation of gut bacteria in the Pacific oyster Magallana gigas and Mediterranean mussel Mytilus galloprovincialis

Feed composition affects the gut bacterial community in aquatic species, but little is known about how diet type impacts gut microbiota in molluscan bivalves. This study investigates the impact of feed composition on the gut bacterial community in two commercially important bivalves: Pacific oyster...

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Main Authors: Shirin Akter, Melissa L. Wos-Oxley, Sarah R. Catalano, Md Mahbubul Hassan, Xiaoxu Li, Andrew P.A. Oxley, Jian G. Qin
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2025-09-01
Series:Aquaculture and Fisheries
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Online Access:http://www.sciencedirect.com/science/article/pii/S2468550X24001187
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author Shirin Akter
Melissa L. Wos-Oxley
Sarah R. Catalano
Md Mahbubul Hassan
Xiaoxu Li
Andrew P.A. Oxley
Jian G. Qin
author_facet Shirin Akter
Melissa L. Wos-Oxley
Sarah R. Catalano
Md Mahbubul Hassan
Xiaoxu Li
Andrew P.A. Oxley
Jian G. Qin
author_sort Shirin Akter
collection DOAJ
description Feed composition affects the gut bacterial community in aquatic species, but little is known about how diet type impacts gut microbiota in molluscan bivalves. This study investigates the impact of feed composition on the gut bacterial community in two commercially important bivalves: Pacific oyster Magallana gigas and Mediterranean mussel Mytilus galloprovincialis. Oysters and mussels were fed with two types of marine algae, live Isochrysis galbana (microalgae) and the dried seaweed powder Ulva sp. (macroalgae) under a laboratory condition for two months. The V1–V2 region of the 16S rDNA was sequenced through an Illumina MiSeq platform to compare the gut bacterial community of oysters and mussels at the start and the end of the feeding trial. Feed composition directly impacted both alpha and beta diversities of gut microbiota, but the degree of the impact differed between bivalve species. The difference in the gut bacterial assemblages of oysters and mussels in the pre- and post-feeding trials indicated that the diet type and host species shape the composition of gut bacteria. Spirochaeta was absent in mussel guts during the pre- and post-feeding trials but was present in oysters, revealing host specificity in colonizing gut bacteria. The results suggest that the diet type and host species can significantly impact the gut bacterial community structure. This study confirms that diet can modulate gut microbial composition in marine filter-feeding organisms, but the extent of dietary modulation depends on feed type and host species.
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spelling doaj-art-c99baa7f79b34994b73dda3e63edee0b2025-08-22T04:57:02ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2025-09-0110585285910.1016/j.aaf.2024.08.001Dietary regulation of gut bacteria in the Pacific oyster Magallana gigas and Mediterranean mussel Mytilus galloprovincialisShirin Akter0Melissa L. Wos-Oxley1Sarah R. Catalano2Md Mahbubul Hassan3Xiaoxu Li4Andrew P.A. Oxley5Jian G. Qin6College of Science and Engineering, Flinders University, Adelaide, 5001, SA, AustraliaSchool of Medicine, Deakin University, Geelong, 3216, VIC, AustraliaAquatic Sciences Centre, South Australian Research and Development Institute, West Beach, 5024, SA, AustraliaAquaculture Research and Development, Department of Primary Industries and Regional Development, Hillarys, 6025, WA, AustraliaAquatic Sciences Centre, South Australian Research and Development Institute, West Beach, 5024, SA, AustraliaSchool of Life and Environmental Sciences, Deakin University, Geelong, 3216, VIC, AustraliaCollege of Science and Engineering, Flinders University, Adelaide, 5001, SA, Australia; Corresponding author.Feed composition affects the gut bacterial community in aquatic species, but little is known about how diet type impacts gut microbiota in molluscan bivalves. This study investigates the impact of feed composition on the gut bacterial community in two commercially important bivalves: Pacific oyster Magallana gigas and Mediterranean mussel Mytilus galloprovincialis. Oysters and mussels were fed with two types of marine algae, live Isochrysis galbana (microalgae) and the dried seaweed powder Ulva sp. (macroalgae) under a laboratory condition for two months. The V1–V2 region of the 16S rDNA was sequenced through an Illumina MiSeq platform to compare the gut bacterial community of oysters and mussels at the start and the end of the feeding trial. Feed composition directly impacted both alpha and beta diversities of gut microbiota, but the degree of the impact differed between bivalve species. The difference in the gut bacterial assemblages of oysters and mussels in the pre- and post-feeding trials indicated that the diet type and host species shape the composition of gut bacteria. Spirochaeta was absent in mussel guts during the pre- and post-feeding trials but was present in oysters, revealing host specificity in colonizing gut bacteria. The results suggest that the diet type and host species can significantly impact the gut bacterial community structure. This study confirms that diet can modulate gut microbial composition in marine filter-feeding organisms, but the extent of dietary modulation depends on feed type and host species.http://www.sciencedirect.com/science/article/pii/S2468550X24001187DietGut microbiotaOystersMusselsUlva powderMacroalgal debris
spellingShingle Shirin Akter
Melissa L. Wos-Oxley
Sarah R. Catalano
Md Mahbubul Hassan
Xiaoxu Li
Andrew P.A. Oxley
Jian G. Qin
Dietary regulation of gut bacteria in the Pacific oyster Magallana gigas and Mediterranean mussel Mytilus galloprovincialis
Aquaculture and Fisheries
Diet
Gut microbiota
Oysters
Mussels
Ulva powder
Macroalgal debris
title Dietary regulation of gut bacteria in the Pacific oyster Magallana gigas and Mediterranean mussel Mytilus galloprovincialis
title_full Dietary regulation of gut bacteria in the Pacific oyster Magallana gigas and Mediterranean mussel Mytilus galloprovincialis
title_fullStr Dietary regulation of gut bacteria in the Pacific oyster Magallana gigas and Mediterranean mussel Mytilus galloprovincialis
title_full_unstemmed Dietary regulation of gut bacteria in the Pacific oyster Magallana gigas and Mediterranean mussel Mytilus galloprovincialis
title_short Dietary regulation of gut bacteria in the Pacific oyster Magallana gigas and Mediterranean mussel Mytilus galloprovincialis
title_sort dietary regulation of gut bacteria in the pacific oyster magallana gigas and mediterranean mussel mytilus galloprovincialis
topic Diet
Gut microbiota
Oysters
Mussels
Ulva powder
Macroalgal debris
url http://www.sciencedirect.com/science/article/pii/S2468550X24001187
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