Metabolic phenotype of bovine blood-derived neutrophils is altered in milk
Abstract In a healthy udder, immune cells from the peripheral bloodstream migrate into mammary tissue in low numbers to provide baseline immune surveillance, without triggering inflammation. In bovine intramammary inflammation, on the other hand, high amounts of leukocytes are recruited, causing sev...
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| Format: | Article |
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Nature Portfolio
2025-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-93929-y |
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| author | Heidi C. Duda Carolin J. Sprenzel Andrea Didier Armin M. Scholz Cornelia A. Deeg Roxane L. Degroote |
| author_facet | Heidi C. Duda Carolin J. Sprenzel Andrea Didier Armin M. Scholz Cornelia A. Deeg Roxane L. Degroote |
| author_sort | Heidi C. Duda |
| collection | DOAJ |
| description | Abstract In a healthy udder, immune cells from the peripheral bloodstream migrate into mammary tissue in low numbers to provide baseline immune surveillance, without triggering inflammation. In bovine intramammary inflammation, on the other hand, high amounts of leukocytes are recruited, causing severe inflammation. We were interested in leukocyte subpopulations and functional differences between blood- and milk-derived neutrophils from healthy and inflamed udder quarters. In this context, we found a distinct leukocyte subpopulation profile dependent on the health status of mammary gland quarters, with a predominant T cells population in heathy mammary gland quarters and a shift to macrophages and granulocytes in inflammation. Further, we detected divergent expression of major histocompatibility complex class II and interleukin 2 receptor CD25 on the surface of milk- and blood-derived neutrophils, pointing to antigen presentation and immune modulatory properties. Moreover, we observed differences in production of reactive oxygen species, deviant early and late apoptosis and functional changes in these cells, pointing to an altered metabolic phenotype in milk cells dependent on the health status of mammary gland quarters. These findings provide insights into the functional adaptations of neutrophils in different environments, highlighting the importance of metabolic alterations for immune cell function. |
| format | Article |
| id | doaj-art-c99ac394f1ef4268a81706c71da02fdd |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-c99ac394f1ef4268a81706c71da02fdd2025-08-20T03:41:41ZengNature PortfolioScientific Reports2045-23222025-03-0115111310.1038/s41598-025-93929-yMetabolic phenotype of bovine blood-derived neutrophils is altered in milkHeidi C. Duda0Carolin J. Sprenzel1Andrea Didier2Armin M. Scholz3Cornelia A. Deeg4Roxane L. Degroote5Chair of Physiology, Department of Veterinary Sciences, Ludwig-Maximilians-University MunichChair of Physiology, Department of Veterinary Sciences, Ludwig-Maximilians-University MunichChair of Hygiene and Technology of Milk, Department of Veterinary Sciences, Ludwig-Maximilians-University MunichLivestock Center of the Faculty of Veterinary Medicine, Ludwig-Maximilians-University MunichChair of Physiology, Department of Veterinary Sciences, Ludwig-Maximilians-University MunichChair of Physiology, Department of Veterinary Sciences, Ludwig-Maximilians-University MunichAbstract In a healthy udder, immune cells from the peripheral bloodstream migrate into mammary tissue in low numbers to provide baseline immune surveillance, without triggering inflammation. In bovine intramammary inflammation, on the other hand, high amounts of leukocytes are recruited, causing severe inflammation. We were interested in leukocyte subpopulations and functional differences between blood- and milk-derived neutrophils from healthy and inflamed udder quarters. In this context, we found a distinct leukocyte subpopulation profile dependent on the health status of mammary gland quarters, with a predominant T cells population in heathy mammary gland quarters and a shift to macrophages and granulocytes in inflammation. Further, we detected divergent expression of major histocompatibility complex class II and interleukin 2 receptor CD25 on the surface of milk- and blood-derived neutrophils, pointing to antigen presentation and immune modulatory properties. Moreover, we observed differences in production of reactive oxygen species, deviant early and late apoptosis and functional changes in these cells, pointing to an altered metabolic phenotype in milk cells dependent on the health status of mammary gland quarters. These findings provide insights into the functional adaptations of neutrophils in different environments, highlighting the importance of metabolic alterations for immune cell function.https://doi.org/10.1038/s41598-025-93929-y |
| spellingShingle | Heidi C. Duda Carolin J. Sprenzel Andrea Didier Armin M. Scholz Cornelia A. Deeg Roxane L. Degroote Metabolic phenotype of bovine blood-derived neutrophils is altered in milk Scientific Reports |
| title | Metabolic phenotype of bovine blood-derived neutrophils is altered in milk |
| title_full | Metabolic phenotype of bovine blood-derived neutrophils is altered in milk |
| title_fullStr | Metabolic phenotype of bovine blood-derived neutrophils is altered in milk |
| title_full_unstemmed | Metabolic phenotype of bovine blood-derived neutrophils is altered in milk |
| title_short | Metabolic phenotype of bovine blood-derived neutrophils is altered in milk |
| title_sort | metabolic phenotype of bovine blood derived neutrophils is altered in milk |
| url | https://doi.org/10.1038/s41598-025-93929-y |
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