Role of TGF-β1/SMAD signaling cascade in the regulation of cyclooxygenase-2 expression in human breast cancer cells

The expression of the SMAD2, SMAD4, SMAD7, and СOX-2 genes and the levels of Smad4, phosphorylated Smad2, and Smad7 proteins were analyzed in the human breast cancer (BC) cell lines MCF-7, BT-474, and ZR-75-1. The SMAD2 and SMAD4 genes were found to suppress COX-2 gene expression in the estrogen rec...

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Main Authors: M. A. Taipov, Z. N. Nikiforova, O. M. Pavlova, I. A. Kudryavtsev, N. Ye. Arnotskaya, V. Ye. Shevchenko
Format: Article
Language:Russian
Published: ABV-press 2014-07-01
Series:Опухоли женской репродуктивной системы
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Online Access:https://ojrs.abvpress.ru/ojrs/article/view/33
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author M. A. Taipov
Z. N. Nikiforova
O. M. Pavlova
I. A. Kudryavtsev
N. Ye. Arnotskaya
V. Ye. Shevchenko
author_facet M. A. Taipov
Z. N. Nikiforova
O. M. Pavlova
I. A. Kudryavtsev
N. Ye. Arnotskaya
V. Ye. Shevchenko
author_sort M. A. Taipov
collection DOAJ
description The expression of the SMAD2, SMAD4, SMAD7, and СOX-2 genes and the levels of Smad4, phosphorylated Smad2, and Smad7 proteins were analyzed in the human breast cancer (BC) cell lines MCF-7, BT-474, and ZR-75-1. The SMAD2 and SMAD4 genes were found to suppress COX-2 gene expression in the estrogen receptor-positive BC cells and to be linked to their metastatic potential. Smad4 and Smad2 apparently suppress COX-2 mRNA and protein synthesis and Smad7 is their antagonist and stimulates COX-2 formation. Smad2, Smad4, Smad7 proteins may be regarded as new potential targets for targeted BC therapy.
format Article
id doaj-art-c98ca73b0720479eb0a96d9ba11d1b63
institution Kabale University
issn 1994-4098
1999-8627
language Russian
publishDate 2014-07-01
publisher ABV-press
record_format Article
series Опухоли женской репродуктивной системы
spelling doaj-art-c98ca73b0720479eb0a96d9ba11d1b632025-08-20T04:00:02ZrusABV-pressОпухоли женской репродуктивной системы1994-40981999-86272014-07-0101-261210.17650/1994-4098-2013-0-1-2-6-1248Role of TGF-β1/SMAD signaling cascade in the regulation of cyclooxygenase-2 expression in human breast cancer cellsM. A. Taipov0Z. N. Nikiforova1O. M. Pavlova2I. A. Kudryavtsev3N. Ye. Arnotskaya4V. Ye. Shevchenko5N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowN.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowN.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowN.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowN.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowN.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowThe expression of the SMAD2, SMAD4, SMAD7, and СOX-2 genes and the levels of Smad4, phosphorylated Smad2, and Smad7 proteins were analyzed in the human breast cancer (BC) cell lines MCF-7, BT-474, and ZR-75-1. The SMAD2 and SMAD4 genes were found to suppress COX-2 gene expression in the estrogen receptor-positive BC cells and to be linked to their metastatic potential. Smad4 and Smad2 apparently suppress COX-2 mRNA and protein synthesis and Smad7 is their antagonist and stimulates COX-2 formation. Smad2, Smad4, Smad7 proteins may be regarded as new potential targets for targeted BC therapy.https://ojrs.abvpress.ru/ojrs/article/view/33breast cancercyclooxygenase-2 (cox-2)tgf-β1/smad signaling pathwaysmad2smad4and smad7 genessmad4smad2and smad7 proteinsphosphorylated smad2inhibitors of cox-2pge2human breast cancer cell lines mcf-7bt-474and zr- 75-1targets f
spellingShingle M. A. Taipov
Z. N. Nikiforova
O. M. Pavlova
I. A. Kudryavtsev
N. Ye. Arnotskaya
V. Ye. Shevchenko
Role of TGF-β1/SMAD signaling cascade in the regulation of cyclooxygenase-2 expression in human breast cancer cells
Опухоли женской репродуктивной системы
breast cancer
cyclooxygenase-2 (cox-2)
tgf-β1/smad signaling pathway
smad2
smad4
and smad7 genes
smad4
smad2
and smad7 proteins
phosphorylated smad2
inhibitors of cox-2
pge2
human breast cancer cell lines mcf-7
bt-474
and zr- 75-1
targets f
title Role of TGF-β1/SMAD signaling cascade in the regulation of cyclooxygenase-2 expression in human breast cancer cells
title_full Role of TGF-β1/SMAD signaling cascade in the regulation of cyclooxygenase-2 expression in human breast cancer cells
title_fullStr Role of TGF-β1/SMAD signaling cascade in the regulation of cyclooxygenase-2 expression in human breast cancer cells
title_full_unstemmed Role of TGF-β1/SMAD signaling cascade in the regulation of cyclooxygenase-2 expression in human breast cancer cells
title_short Role of TGF-β1/SMAD signaling cascade in the regulation of cyclooxygenase-2 expression in human breast cancer cells
title_sort role of tgf β1 smad signaling cascade in the regulation of cyclooxygenase 2 expression in human breast cancer cells
topic breast cancer
cyclooxygenase-2 (cox-2)
tgf-β1/smad signaling pathway
smad2
smad4
and smad7 genes
smad4
smad2
and smad7 proteins
phosphorylated smad2
inhibitors of cox-2
pge2
human breast cancer cell lines mcf-7
bt-474
and zr- 75-1
targets f
url https://ojrs.abvpress.ru/ojrs/article/view/33
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