Suppressive effects of co-stimulatory molecule expressions on mouse splenocytes by anti-allergic agents in vitro
The influence of anti-allergic drugs, epinastine hydrochloride (EP) and disodium cromoglycate (DSCG), on the co-stimulatory molecule expression was examined using in vitro cell culture technique. Spleen cells obtained from BALB/c mice 10 days after immunization with haemocyanin absorbed to aluminium...
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| Format: | Article |
| Language: | English |
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Wiley
2000-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/096293500411523 |
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| author | Jun-ichi Ito Kazuhito Asano Elzbieta Tryka Ken-ichi Kanai Sumiko Yamamoto Tadashi Hisamitsu Harumi Suzaki |
| author_facet | Jun-ichi Ito Kazuhito Asano Elzbieta Tryka Ken-ichi Kanai Sumiko Yamamoto Tadashi Hisamitsu Harumi Suzaki |
| author_sort | Jun-ichi Ito |
| collection | DOAJ |
| description | The influence of anti-allergic drugs, epinastine hydrochloride (EP) and disodium cromoglycate (DSCG), on the co-stimulatory molecule expression was examined using in vitro cell culture technique. Spleen cells obtained from BALB/c mice 10 days after immunization with haemocyanin absorbed to aluminium hydroxide were cultured in the presence of 100.0 μg/ml haemocyanin and various concentrations of the agents. Low concentrations (< 1.5 2 10-4 M) of EP and DSCG did not influence spleen cell blastic activity induced by antigenic stimulation, whereas these agents caused significant inhibition of spleen cell activation when 2 × 10-4 M of the agents were added to cell cultures. EP and DSCG also did not affect blastic activity of sensitized splenic T cells by anti-CD3 monoclonal antibody stimulation even when these cells were cultured in the presence of 2 × 10-4 M of the agents. We next examined the influence of EP and DSCG on the expression of co-stimulatory molecules on spleen cells in response to antigenic stimulation. Sensitized spleen cells were cultured in the presence of 2 × 10-4 M of the agents and the expression of molecules were examined by flow cytometer 24 h later. EP and DSCG suppressed the expression of costimulatory molecules, CD40 and CD80, but not CD86, on splenic B cells which were enhanced by antigenic stimulation in vitro. |
| format | Article |
| id | doaj-art-c982737fe74f49fabdac4b1ab7db03e2 |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-c982737fe74f49fabdac4b1ab7db03e22025-08-20T03:24:03ZengWileyMediators of Inflammation0962-93511466-18612000-01-0192697510.1080/096293500411523Suppressive effects of co-stimulatory molecule expressions on mouse splenocytes by anti-allergic agents in vitroJun-ichi Ito0Kazuhito Asano1Elzbieta Tryka2Ken-ichi Kanai3Sumiko Yamamoto4Tadashi Hisamitsu5Harumi Suzaki6Department of Otolaryngology, School of Medicine, Showa University, Tokyo, JapanDepartment of Physiology, School of Medicine, Showa University, Tokyo, JapanDepartment of Otolaryngology, School of Medicine, Showa University, Tokyo, JapanDepartment of Otolaryngology, School of Medicine, Showa University, Tokyo, JapanDepartment of Otolaryngology, School of Medicine, Showa University, Tokyo, JapanDepartment of Physiology, School of Medicine, Showa University, Tokyo, JapanDepartment of Otolaryngology, School of Medicine, Showa University, Tokyo, JapanThe influence of anti-allergic drugs, epinastine hydrochloride (EP) and disodium cromoglycate (DSCG), on the co-stimulatory molecule expression was examined using in vitro cell culture technique. Spleen cells obtained from BALB/c mice 10 days after immunization with haemocyanin absorbed to aluminium hydroxide were cultured in the presence of 100.0 μg/ml haemocyanin and various concentrations of the agents. Low concentrations (< 1.5 2 10-4 M) of EP and DSCG did not influence spleen cell blastic activity induced by antigenic stimulation, whereas these agents caused significant inhibition of spleen cell activation when 2 × 10-4 M of the agents were added to cell cultures. EP and DSCG also did not affect blastic activity of sensitized splenic T cells by anti-CD3 monoclonal antibody stimulation even when these cells were cultured in the presence of 2 × 10-4 M of the agents. We next examined the influence of EP and DSCG on the expression of co-stimulatory molecules on spleen cells in response to antigenic stimulation. Sensitized spleen cells were cultured in the presence of 2 × 10-4 M of the agents and the expression of molecules were examined by flow cytometer 24 h later. EP and DSCG suppressed the expression of costimulatory molecules, CD40 and CD80, but not CD86, on splenic B cells which were enhanced by antigenic stimulation in vitro.http://dx.doi.org/10.1080/096293500411523 |
| spellingShingle | Jun-ichi Ito Kazuhito Asano Elzbieta Tryka Ken-ichi Kanai Sumiko Yamamoto Tadashi Hisamitsu Harumi Suzaki Suppressive effects of co-stimulatory molecule expressions on mouse splenocytes by anti-allergic agents in vitro Mediators of Inflammation |
| title | Suppressive effects of co-stimulatory molecule expressions on mouse splenocytes by anti-allergic agents in vitro |
| title_full | Suppressive effects of co-stimulatory molecule expressions on mouse splenocytes by anti-allergic agents in vitro |
| title_fullStr | Suppressive effects of co-stimulatory molecule expressions on mouse splenocytes by anti-allergic agents in vitro |
| title_full_unstemmed | Suppressive effects of co-stimulatory molecule expressions on mouse splenocytes by anti-allergic agents in vitro |
| title_short | Suppressive effects of co-stimulatory molecule expressions on mouse splenocytes by anti-allergic agents in vitro |
| title_sort | suppressive effects of co stimulatory molecule expressions on mouse splenocytes by anti allergic agents in vitro |
| url | http://dx.doi.org/10.1080/096293500411523 |
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