Optimization of methanol‐induced expression and His‐tag purification of Saccharomycopsis fibuligera R64 mutant α‐amylase in Pichia pastoris

Optimization of methanol‐induced expression and His‐tag purification of Saccharomycopsis fibuligera R64 mutant α‐amylase in Pichia pastoris

The Sfamy R64 α‐amylase mutant from Saccharomycopsis fibuligera was expressed in Pichia pastoris to explore its industrial potential. The gene encoding the mutant enzyme was cloned into the pPICZαA vector and transformed into P. pastoris SMD1168. Optimal expression was achieved at 1.5% methanol conc...

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Bibliographic Details
Main Authors: Clara Claudia, Elsa Destiana, Rista Awalia, Mia Tria Novianti, Taufik Ramdani Tohari, Dewi Astriany, Shinta Kusumawardani, Muhammad Yusuf, Umi Baroroh
Format: Article
Language:English
Published: Universitas Gadjah Mada, Yogyakarta 2025-06-01
Series:Indonesian Journal of Biotechnology
Subjects:
affinity chromatography
md simulation
methanol induction
pichia pastoris
sfamy r64 mutant
Online Access:https://jurnal.ugm.ac.id/ijbiotech/article/view/100845
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