Complete biosynthetic pathway of furochromones in Saposhnikovia divaricata and its evolutionary mechanism in Apiaceae plants
Abstract Furochromones are specific bioactive secondary metabolites of many Apiaceae plants. Their biosynthesis remains largely unexplored. In this work, we dissect the complete biosynthetic pathway of major furochromones in the medicinal plant Saposhnikovia divaricata by characterizing prenyltransf...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-04-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-58498-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850258873336725504 |
|---|---|
| author | Jian-lin Zou Hong-ye Li Bao Nie Zi-long Wang Chun-xue Zhao Yun-gang Tian Li-qun Lin Wei-zhe Xu Zhuang-wei Hou Wen-kai Sun Xiao-xu Han Meng Zhang Hao-tian Wang Qing-yan Li Li Wang Min Ye |
| author_facet | Jian-lin Zou Hong-ye Li Bao Nie Zi-long Wang Chun-xue Zhao Yun-gang Tian Li-qun Lin Wei-zhe Xu Zhuang-wei Hou Wen-kai Sun Xiao-xu Han Meng Zhang Hao-tian Wang Qing-yan Li Li Wang Min Ye |
| author_sort | Jian-lin Zou |
| collection | DOAJ |
| description | Abstract Furochromones are specific bioactive secondary metabolites of many Apiaceae plants. Their biosynthesis remains largely unexplored. In this work, we dissect the complete biosynthetic pathway of major furochromones in the medicinal plant Saposhnikovia divaricata by characterizing prenyltransferase, peucenin cyclase, methyltransferase, hydroxylase, and glycosyltransferases. De novo biosynthesis of prim-O-glucosylcimifugin and 5-O-methylvisamminoside is realized in Nicotiana benthamiana leaves. Through comparative genomic and transcriptomic analyses, we further find that proximal duplication and high expression of a pentaketide chromone synthase gene SdPCS, together with the presence of a lineage-specific peucenin cyclase gene SdPC, lead to the predominant accumulation of furochromones in the roots of S. divaricata among surveyed Apiaceae plants. This study paves the way for metabolic engineering production of furochromones, and sheds light into evolutionary mechanisms of furochromone biosynthesis among Apiaceae plants. |
| format | Article |
| id | doaj-art-c9757fe45abb4977b090053b8b6836db |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-c9757fe45abb4977b090053b8b6836db2025-08-20T01:56:01ZengNature PortfolioNature Communications2041-17232025-04-0116111510.1038/s41467-025-58498-8Complete biosynthetic pathway of furochromones in Saposhnikovia divaricata and its evolutionary mechanism in Apiaceae plantsJian-lin Zou0Hong-ye Li1Bao Nie2Zi-long Wang3Chun-xue Zhao4Yun-gang Tian5Li-qun Lin6Wei-zhe Xu7Zhuang-wei Hou8Wen-kai Sun9Xiao-xu Han10Meng Zhang11Hao-tian Wang12Qing-yan Li13Li Wang14Min Ye15State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural SciencesState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural SciencesCivil Aviation Medicine Center, Civil Aviation Administration of China, A-1 GaojingShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural SciencesShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural SciencesShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural SciencesState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityCivil Aviation Medicine Center, Civil Aviation Administration of China, A-1 GaojingShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural SciencesState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityAbstract Furochromones are specific bioactive secondary metabolites of many Apiaceae plants. Their biosynthesis remains largely unexplored. In this work, we dissect the complete biosynthetic pathway of major furochromones in the medicinal plant Saposhnikovia divaricata by characterizing prenyltransferase, peucenin cyclase, methyltransferase, hydroxylase, and glycosyltransferases. De novo biosynthesis of prim-O-glucosylcimifugin and 5-O-methylvisamminoside is realized in Nicotiana benthamiana leaves. Through comparative genomic and transcriptomic analyses, we further find that proximal duplication and high expression of a pentaketide chromone synthase gene SdPCS, together with the presence of a lineage-specific peucenin cyclase gene SdPC, lead to the predominant accumulation of furochromones in the roots of S. divaricata among surveyed Apiaceae plants. This study paves the way for metabolic engineering production of furochromones, and sheds light into evolutionary mechanisms of furochromone biosynthesis among Apiaceae plants.https://doi.org/10.1038/s41467-025-58498-8 |
| spellingShingle | Jian-lin Zou Hong-ye Li Bao Nie Zi-long Wang Chun-xue Zhao Yun-gang Tian Li-qun Lin Wei-zhe Xu Zhuang-wei Hou Wen-kai Sun Xiao-xu Han Meng Zhang Hao-tian Wang Qing-yan Li Li Wang Min Ye Complete biosynthetic pathway of furochromones in Saposhnikovia divaricata and its evolutionary mechanism in Apiaceae plants Nature Communications |
| title | Complete biosynthetic pathway of furochromones in Saposhnikovia divaricata and its evolutionary mechanism in Apiaceae plants |
| title_full | Complete biosynthetic pathway of furochromones in Saposhnikovia divaricata and its evolutionary mechanism in Apiaceae plants |
| title_fullStr | Complete biosynthetic pathway of furochromones in Saposhnikovia divaricata and its evolutionary mechanism in Apiaceae plants |
| title_full_unstemmed | Complete biosynthetic pathway of furochromones in Saposhnikovia divaricata and its evolutionary mechanism in Apiaceae plants |
| title_short | Complete biosynthetic pathway of furochromones in Saposhnikovia divaricata and its evolutionary mechanism in Apiaceae plants |
| title_sort | complete biosynthetic pathway of furochromones in saposhnikovia divaricata and its evolutionary mechanism in apiaceae plants |
| url | https://doi.org/10.1038/s41467-025-58498-8 |
| work_keys_str_mv | AT jianlinzou completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT hongyeli completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT baonie completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT zilongwang completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT chunxuezhao completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT yungangtian completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT liqunlin completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT weizhexu completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT zhuangweihou completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT wenkaisun completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT xiaoxuhan completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT mengzhang completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT haotianwang completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT qingyanli completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT liwang completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants AT minye completebiosyntheticpathwayoffurochromonesinsaposhnikoviadivaricataanditsevolutionarymechanisminapiaceaeplants |