Gene Expression Profiling for In Silico Microdissection of Hodgkin's Lymphoma Microenvironment and Identification of Prognostic Features

Gene expression profiling studies based on DNA microarrays have demonstrated their ability to define the interaction pathways between neoplastic and nonmalignant stromal cells in cancer tissues. During the past ten years, a number of approaches including microdissection have tried to resolve the var...

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Main Authors: François Bertucci, Bruno Chetaille, Luc Xerri
Format: Article
Language:English
Published: Wiley 2011-01-01
Series:Advances in Hematology
Online Access:http://dx.doi.org/10.1155/2011/485310
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author François Bertucci
Bruno Chetaille
Luc Xerri
author_facet François Bertucci
Bruno Chetaille
Luc Xerri
author_sort François Bertucci
collection DOAJ
description Gene expression profiling studies based on DNA microarrays have demonstrated their ability to define the interaction pathways between neoplastic and nonmalignant stromal cells in cancer tissues. During the past ten years, a number of approaches including microdissection have tried to resolve the variability in DNA microarray measurements stemming from cancer tissue sample heterogeneity. Another approach, designated as virtual or in silico microdissection, avoids the laborious and time-consuming step of anatomic microdissection. It consists of confronting the gene expression profiles of complex tissue samples to those of cell lines representative of different cell lineages, different differentiation stages, or different signaling pathways. This strategy has been used in recent studies aiming to analyze microenvironment alterations using gene expression profiling of nonmicrodissected classical Hodgkin lymphoma tissues in order to generate new prognostic factors. These recent contributions are detailed and discussed in the present paper.
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spelling doaj-art-c9646acb9abb468dbbe2785fe6dc211b2025-08-20T03:19:34ZengWileyAdvances in Hematology1687-91041687-91122011-01-01201110.1155/2011/485310485310Gene Expression Profiling for In Silico Microdissection of Hodgkin's Lymphoma Microenvironment and Identification of Prognostic FeaturesFrançois Bertucci0Bruno Chetaille1Luc Xerri2Department of Bio-Pathology, Institut Paoli-Calmettes, 232, bd Sainte-Marguerite, 13273 Marseille Cedex 09, FranceDepartment of Bio-Pathology, Institut Paoli-Calmettes, 232, bd Sainte-Marguerite, 13273 Marseille Cedex 09, FranceDepartment of Bio-Pathology, Institut Paoli-Calmettes, 232, bd Sainte-Marguerite, 13273 Marseille Cedex 09, FranceGene expression profiling studies based on DNA microarrays have demonstrated their ability to define the interaction pathways between neoplastic and nonmalignant stromal cells in cancer tissues. During the past ten years, a number of approaches including microdissection have tried to resolve the variability in DNA microarray measurements stemming from cancer tissue sample heterogeneity. Another approach, designated as virtual or in silico microdissection, avoids the laborious and time-consuming step of anatomic microdissection. It consists of confronting the gene expression profiles of complex tissue samples to those of cell lines representative of different cell lineages, different differentiation stages, or different signaling pathways. This strategy has been used in recent studies aiming to analyze microenvironment alterations using gene expression profiling of nonmicrodissected classical Hodgkin lymphoma tissues in order to generate new prognostic factors. These recent contributions are detailed and discussed in the present paper.http://dx.doi.org/10.1155/2011/485310
spellingShingle François Bertucci
Bruno Chetaille
Luc Xerri
Gene Expression Profiling for In Silico Microdissection of Hodgkin's Lymphoma Microenvironment and Identification of Prognostic Features
Advances in Hematology
title Gene Expression Profiling for In Silico Microdissection of Hodgkin's Lymphoma Microenvironment and Identification of Prognostic Features
title_full Gene Expression Profiling for In Silico Microdissection of Hodgkin's Lymphoma Microenvironment and Identification of Prognostic Features
title_fullStr Gene Expression Profiling for In Silico Microdissection of Hodgkin's Lymphoma Microenvironment and Identification of Prognostic Features
title_full_unstemmed Gene Expression Profiling for In Silico Microdissection of Hodgkin's Lymphoma Microenvironment and Identification of Prognostic Features
title_short Gene Expression Profiling for In Silico Microdissection of Hodgkin's Lymphoma Microenvironment and Identification of Prognostic Features
title_sort gene expression profiling for in silico microdissection of hodgkin s lymphoma microenvironment and identification of prognostic features
url http://dx.doi.org/10.1155/2011/485310
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