Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers.
Type VII collagen, as a major component of anchoring fibrils found at basement membrane zones, is crucial in anchoring epithelial tissue layers to their underlying stroma. Recently, type VII collagen was discovered in the inner human retina by means of immunohistochemistry, while proteomic investiga...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145502&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850189420152487936 |
|---|---|
| author | Bart Wullink Hendri H Pas Roelofje J Van der Worp Roel Kuijer Leonoor I Los |
| author_facet | Bart Wullink Hendri H Pas Roelofje J Van der Worp Roel Kuijer Leonoor I Los |
| author_sort | Bart Wullink |
| collection | DOAJ |
| description | Type VII collagen, as a major component of anchoring fibrils found at basement membrane zones, is crucial in anchoring epithelial tissue layers to their underlying stroma. Recently, type VII collagen was discovered in the inner human retina by means of immunohistochemistry, while proteomic investigations demonstrated type VII collagen at the vitreoretinal interface of chicken. Because of its potential anchoring function at the vitreoretinal interface, we further assessed the presence of type VII collagen at this site. We evaluated the vitreoretinal interface of human donor eyes by means of immunohistochemistry, confocal microscopy, immunoelectron microscopy, and Western blotting. Firstly, type VII collagen was detected alongside vitreous fibers6 at the vitreoretinal interface. Because of its known anchoring function, it is likely that type VII collagen is involved in vitreoretinal attachment. Secondly, type VII collagen was found within cytoplasmic vesicles of inner retinal cells. These cells resided most frequently in the ganglion cell layer and inner plexiform layer. Thirdly, type VII collagen was found in astrocytic cytoplasmic inclusions, known as corpora amylacea. The intraretinal presence of type VII collagen was confirmed by Western blotting of homogenized retinal preparations. These data add to the understanding of vitreoretinal attachment, which is important for a better comprehension of common vitreoretinal attachment pathologies. |
| format | Article |
| id | doaj-art-c9616bb2d6144a24869c458b756fa5ee |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-c9616bb2d6144a24869c458b756fa5ee2025-08-20T02:15:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014550210.1371/journal.pone.0145502Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers.Bart WullinkHendri H PasRoelofje J Van der WorpRoel KuijerLeonoor I LosType VII collagen, as a major component of anchoring fibrils found at basement membrane zones, is crucial in anchoring epithelial tissue layers to their underlying stroma. Recently, type VII collagen was discovered in the inner human retina by means of immunohistochemistry, while proteomic investigations demonstrated type VII collagen at the vitreoretinal interface of chicken. Because of its potential anchoring function at the vitreoretinal interface, we further assessed the presence of type VII collagen at this site. We evaluated the vitreoretinal interface of human donor eyes by means of immunohistochemistry, confocal microscopy, immunoelectron microscopy, and Western blotting. Firstly, type VII collagen was detected alongside vitreous fibers6 at the vitreoretinal interface. Because of its known anchoring function, it is likely that type VII collagen is involved in vitreoretinal attachment. Secondly, type VII collagen was found within cytoplasmic vesicles of inner retinal cells. These cells resided most frequently in the ganglion cell layer and inner plexiform layer. Thirdly, type VII collagen was found in astrocytic cytoplasmic inclusions, known as corpora amylacea. The intraretinal presence of type VII collagen was confirmed by Western blotting of homogenized retinal preparations. These data add to the understanding of vitreoretinal attachment, which is important for a better comprehension of common vitreoretinal attachment pathologies.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145502&type=printable |
| spellingShingle | Bart Wullink Hendri H Pas Roelofje J Van der Worp Roel Kuijer Leonoor I Los Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers. PLoS ONE |
| title | Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers. |
| title_full | Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers. |
| title_fullStr | Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers. |
| title_full_unstemmed | Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers. |
| title_short | Type VII Collagen Expression in the Human Vitreoretinal Interface, Corpora Amylacea and Inner Retinal Layers. |
| title_sort | type vii collagen expression in the human vitreoretinal interface corpora amylacea and inner retinal layers |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145502&type=printable |
| work_keys_str_mv | AT bartwullink typeviicollagenexpressioninthehumanvitreoretinalinterfacecorporaamylaceaandinnerretinallayers AT hendrihpas typeviicollagenexpressioninthehumanvitreoretinalinterfacecorporaamylaceaandinnerretinallayers AT roelofjejvanderworp typeviicollagenexpressioninthehumanvitreoretinalinterfacecorporaamylaceaandinnerretinallayers AT roelkuijer typeviicollagenexpressioninthehumanvitreoretinalinterfacecorporaamylaceaandinnerretinallayers AT leonoorilos typeviicollagenexpressioninthehumanvitreoretinalinterfacecorporaamylaceaandinnerretinallayers |