Evaluation of lyophilized Tanacetum vulgare extract in intraruminal bolus and granule forms for gastrointestinal nematode control in sheep: An in vivo clinical study
Background and Aim: Gastrointestinal nematode infections have a significant impact on the health and productivity of sheep worldwide. Conventional anthelmintics are facing rising resistance, prompting the need for alternative control strategies. Tanacetum vulgare (tansy), a traditionally used antipa...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Veterinary World
2025-07-01
|
| Series: | Veterinary World |
| Subjects: | |
| Online Access: | https://www.veterinaryworld.org/Vol.18/July-2025/19.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850061909844295680 |
|---|---|
| author | Aīda Vanaga Dace Keidāne Alīna Kļaviņa Ivars Lūsis Aija Mālniece Dace Bandere Renāte Teterovska Ance Bārzdiņa Oxana Brante Konstantins Logviss Oskars Radziņš Līga Kovaļčuka |
| author_facet | Aīda Vanaga Dace Keidāne Alīna Kļaviņa Ivars Lūsis Aija Mālniece Dace Bandere Renāte Teterovska Ance Bārzdiņa Oxana Brante Konstantins Logviss Oskars Radziņš Līga Kovaļčuka |
| author_sort | Aīda Vanaga |
| collection | DOAJ |
| description | Background and Aim: Gastrointestinal nematode infections have a significant impact on the health and productivity of sheep worldwide. Conventional anthelmintics are facing rising resistance, prompting the need for alternative control strategies. Tanacetum vulgare (tansy), a traditionally used antiparasitic herb in Latvia, has shown in vitro efficacy but lacks in vivo delivery validation. This study aimed to evaluate the in vivo antiparasitic efficacy and safety of T. vulgare extract administered through novel intraruminal boluses and granules in naturally infected sheep.
Materials and Methods: Thirty female Latvian Darkhead lambs (4–5 months; mean 35 ± 0.8 kg) were randomly assigned to five groups: Two bolus groups (A and B), one granule group, and two controls (negative and positive). Groups A and B received intraruminal boluses with different lyophilized extract formulations, the granule group received powdered T. vulgare mixed with feed, and the positive control received levamisole. Fecal egg counts (FECs), clinical signs, and hematological and biochemical parameters were assessed over 56 days. Disintegration testing simulated rumen conditions.
Results: No adverse clinical or physiological effects were observed. Bolus groups exhibited a more rapid and consistent reduction in strongylid FECs compared to the granule and negative control groups. On day 56, egg counts decreased to 325–358 eggs per gram (EPG) in bolus and granule groups, compared to 533 EPG in the negative control. Hematological and biochemical parameters remained within reference ranges.
Conclusion: T. vulgare extract administered through intraruminal bolus was safe, sustained animal health, and effectively reduced gastrointestinal nematode burden. Granules were less effective, potentially due to reduced palatability. Bolus-based phytotherapy may serve as a sustainable, prophylactic alternative to conventional anthelmintics. |
| format | Article |
| id | doaj-art-c94e552e43df4e0d8bf3e9bc79b03fda |
| institution | DOAJ |
| issn | 0972-8988 2231-0916 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj-art-c94e552e43df4e0d8bf3e9bc79b03fda2025-08-20T02:50:04ZengVeterinary WorldVeterinary World0972-89882231-09162025-07-011871991200110.14202/vetworld.2025.1991-2001Evaluation of lyophilized Tanacetum vulgare extract in intraruminal bolus and granule forms for gastrointestinal nematode control in sheep: An in vivo clinical studyAīda Vanaga0https://orcid.org/0000-0002-8096-6184Dace Keidāne1https://orcid.org/0009-0006-9678-1500Alīna Kļaviņa2https://orcid.org/0000-0001-9843-7304Ivars Lūsis3https://orcid.org/0000-0002-7203-5304Aija Mālniece4https://orcid.org/0000-0002-6404-3822Dace Bandere5https://orcid.org/0000-0001-9144-8799Renāte Teterovska6https://orcid.org/0000-0002-2979-9130Ance Bārzdiņa7https://orcid.org/0000-0002-6973-469XOxana Brante8https://orcid.org/0009-0005-7550-3475Konstantins Logviss9https://orcid.org/0000-0002-4780-0852Oskars Radziņš10https://orcid.org/0000-0002-2443-9582Līga Kovaļčuka11https://orcid.org/0000-0002-2524-7427Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Clinical Institute, Jelgava, LV–3004, Latvia.Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Institute of Food and Environmental Hygiene, Jelgava, LV–3004, Latvia.Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Institute of Food and Environmental Hygiene, Jelgava, LV–3004, Latvia.Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Institute of Food and Environmental Hygiene, Jelgava, LV–3004, Latvia.Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Clinical Institute, Jelgava, LV–3004, Latvia.Riga Stradiņš University, Faculty of Pharmacy, Riga, LV-1007, Latvia; Baltic Biomaterials Centre of Excellence, Headquarters at Riga Technical University, Riga LV-1048, Latvia.Riga Stradiņš University, Faculty of Pharmacy, Riga, LV-1007, Latvia; Baltic Biomaterials Centre of Excellence, Headquarters at Riga Technical University, Riga LV-1048, Latvia.Riga Stradiņš University, Faculty of Pharmacy, Riga, LV-1007, Latvia; Baltic Biomaterials Centre of Excellence, Headquarters at Riga Technical University, Riga LV-1048, Latvia.Riga Stradiņš University, Faculty of Pharmacy, Riga, LV-1007, Latvia; Baltic Biomaterials Centre of Excellence, Headquarters at Riga Technical University, Riga LV-1048, Latvia.Riga Stradiņš University, Faculty of Pharmacy, Riga, LV-1007, Latviap; Baltic Biomaterials Centre of Excellence, Headquarters at Riga Technical University, Riga LV-1048, Latvia.Riga Stradiņš University, Faculty of Pharmacy, Riga, LV-1007, Latvia; Baltic Biomaterials Centre of Excellence, Headquarters at Riga Technical University, Riga LV-1048, Latvia.Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Clinical Institute, Jelgava, LV–3004, Latvia.Background and Aim: Gastrointestinal nematode infections have a significant impact on the health and productivity of sheep worldwide. Conventional anthelmintics are facing rising resistance, prompting the need for alternative control strategies. Tanacetum vulgare (tansy), a traditionally used antiparasitic herb in Latvia, has shown in vitro efficacy but lacks in vivo delivery validation. This study aimed to evaluate the in vivo antiparasitic efficacy and safety of T. vulgare extract administered through novel intraruminal boluses and granules in naturally infected sheep. Materials and Methods: Thirty female Latvian Darkhead lambs (4–5 months; mean 35 ± 0.8 kg) were randomly assigned to five groups: Two bolus groups (A and B), one granule group, and two controls (negative and positive). Groups A and B received intraruminal boluses with different lyophilized extract formulations, the granule group received powdered T. vulgare mixed with feed, and the positive control received levamisole. Fecal egg counts (FECs), clinical signs, and hematological and biochemical parameters were assessed over 56 days. Disintegration testing simulated rumen conditions. Results: No adverse clinical or physiological effects were observed. Bolus groups exhibited a more rapid and consistent reduction in strongylid FECs compared to the granule and negative control groups. On day 56, egg counts decreased to 325–358 eggs per gram (EPG) in bolus and granule groups, compared to 533 EPG in the negative control. Hematological and biochemical parameters remained within reference ranges. Conclusion: T. vulgare extract administered through intraruminal bolus was safe, sustained animal health, and effectively reduced gastrointestinal nematode burden. Granules were less effective, potentially due to reduced palatability. Bolus-based phytotherapy may serve as a sustainable, prophylactic alternative to conventional anthelmintics.https://www.veterinaryworld.org/Vol.18/July-2025/19.pdffecal egg countgastrointestinal nematodesintraruminal bolusphytotherapysheeptanacetum vulgare |
| spellingShingle | Aīda Vanaga Dace Keidāne Alīna Kļaviņa Ivars Lūsis Aija Mālniece Dace Bandere Renāte Teterovska Ance Bārzdiņa Oxana Brante Konstantins Logviss Oskars Radziņš Līga Kovaļčuka Evaluation of lyophilized Tanacetum vulgare extract in intraruminal bolus and granule forms for gastrointestinal nematode control in sheep: An in vivo clinical study Veterinary World fecal egg count gastrointestinal nematodes intraruminal bolus phytotherapy sheep tanacetum vulgare |
| title | Evaluation of lyophilized Tanacetum vulgare extract in intraruminal bolus and granule forms for gastrointestinal nematode control in sheep: An in vivo clinical study |
| title_full | Evaluation of lyophilized Tanacetum vulgare extract in intraruminal bolus and granule forms for gastrointestinal nematode control in sheep: An in vivo clinical study |
| title_fullStr | Evaluation of lyophilized Tanacetum vulgare extract in intraruminal bolus and granule forms for gastrointestinal nematode control in sheep: An in vivo clinical study |
| title_full_unstemmed | Evaluation of lyophilized Tanacetum vulgare extract in intraruminal bolus and granule forms for gastrointestinal nematode control in sheep: An in vivo clinical study |
| title_short | Evaluation of lyophilized Tanacetum vulgare extract in intraruminal bolus and granule forms for gastrointestinal nematode control in sheep: An in vivo clinical study |
| title_sort | evaluation of lyophilized tanacetum vulgare extract in intraruminal bolus and granule forms for gastrointestinal nematode control in sheep an in vivo clinical study |
| topic | fecal egg count gastrointestinal nematodes intraruminal bolus phytotherapy sheep tanacetum vulgare |
| url | https://www.veterinaryworld.org/Vol.18/July-2025/19.pdf |
| work_keys_str_mv | AT aidavanaga evaluationoflyophilizedtanacetumvulgareextractinintraruminalbolusandgranuleformsforgastrointestinalnematodecontrolinsheepaninvivoclinicalstudy AT dacekeidane evaluationoflyophilizedtanacetumvulgareextractinintraruminalbolusandgranuleformsforgastrointestinalnematodecontrolinsheepaninvivoclinicalstudy AT alinaklavina evaluationoflyophilizedtanacetumvulgareextractinintraruminalbolusandgranuleformsforgastrointestinalnematodecontrolinsheepaninvivoclinicalstudy AT ivarslusis evaluationoflyophilizedtanacetumvulgareextractinintraruminalbolusandgranuleformsforgastrointestinalnematodecontrolinsheepaninvivoclinicalstudy AT aijamalniece evaluationoflyophilizedtanacetumvulgareextractinintraruminalbolusandgranuleformsforgastrointestinalnematodecontrolinsheepaninvivoclinicalstudy AT dacebandere evaluationoflyophilizedtanacetumvulgareextractinintraruminalbolusandgranuleformsforgastrointestinalnematodecontrolinsheepaninvivoclinicalstudy AT renateteterovska evaluationoflyophilizedtanacetumvulgareextractinintraruminalbolusandgranuleformsforgastrointestinalnematodecontrolinsheepaninvivoclinicalstudy AT ancebarzdina evaluationoflyophilizedtanacetumvulgareextractinintraruminalbolusandgranuleformsforgastrointestinalnematodecontrolinsheepaninvivoclinicalstudy AT oxanabrante evaluationoflyophilizedtanacetumvulgareextractinintraruminalbolusandgranuleformsforgastrointestinalnematodecontrolinsheepaninvivoclinicalstudy AT konstantinslogviss evaluationoflyophilizedtanacetumvulgareextractinintraruminalbolusandgranuleformsforgastrointestinalnematodecontrolinsheepaninvivoclinicalstudy AT oskarsradzins evaluationoflyophilizedtanacetumvulgareextractinintraruminalbolusandgranuleformsforgastrointestinalnematodecontrolinsheepaninvivoclinicalstudy AT ligakovalcuka evaluationoflyophilizedtanacetumvulgareextractinintraruminalbolusandgranuleformsforgastrointestinalnematodecontrolinsheepaninvivoclinicalstudy |