Alternative splicing dynamics during gastrulation in mouse embryo
Abstract Alternative splicing (AS) plays an essential role in development, differentiation and carcinogenesis. However, the mechanisms underlying splicing regulation during mouse embryo gastrulation remain unclear. Based on spatial-temporal transcriptome and epigenome data, we detected the dynamics...
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Nature Portfolio
2025-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-96148-7 |
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| author | Wei Wang Yu Zhang Yuanyuan Zhai Wuritu Yang Yongqiang Xing |
| author_facet | Wei Wang Yu Zhang Yuanyuan Zhai Wuritu Yang Yongqiang Xing |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | Abstract Alternative splicing (AS) plays an essential role in development, differentiation and carcinogenesis. However, the mechanisms underlying splicing regulation during mouse embryo gastrulation remain unclear. Based on spatial-temporal transcriptome and epigenome data, we detected the dynamics of AS and revealed its regulatory mechanisms across primary germ layers during mouse gastrulation, spanning developmental stages from E6.5 to E7.5. Subsequently, the dynamic expression of splicing factors (SFs) during gastrulation was characterized, while the expression patterns and functions of germ layer-specific SFs were identified. The results indicate that AS and differential alternative splicing events (DASEs) exhibit dynamic changes and are significantly abundant during the late stage of gastrulation. Similarly, SFs demonstrate stage-specific expression, with elevated levels observed during the middle and late stages of gastrulation. Epigenetic signals associated with SFs and AS sites demonstrate significant enrichment and undergo dynamic changes throughout gastrulation. Overall, this study offers a systematic analysis of AS during mouse gastrulation, identifies primary germ layer-specific AS events, and characterizes the expression patterns of SFs and the associated epigenetic signals. These findings enhance the understanding of the mechanisms underlying the formation of the three germ layers during mammalian gastrulation, with a focus on pre-mRNA AS. |
| format | Article |
| id | doaj-art-c94b5243d26c479ba4a8a963bd8e0ef6 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-c94b5243d26c479ba4a8a963bd8e0ef62025-08-20T01:56:06ZengNature PortfolioScientific Reports2045-23222025-03-0115111310.1038/s41598-025-96148-7Alternative splicing dynamics during gastrulation in mouse embryoWei Wang0Yu Zhang1Yuanyuan Zhai2Wuritu Yang3Yongqiang Xing4Inner Mongolia Key Laboratory of Life Health and Bioinformatics, School of Life Science and Technology, Inner Mongolia University of Science and TechnologyInner Mongolia Key Laboratory of Life Health and Bioinformatics, School of Life Science and Technology, Inner Mongolia University of Science and TechnologyInner Mongolia Key Laboratory of Life Health and Bioinformatics, School of Life Science and Technology, Inner Mongolia University of Science and TechnologyComputer Department, Hohhot Vocational CollegeInner Mongolia Key Laboratory of Life Health and Bioinformatics, School of Life Science and Technology, Inner Mongolia University of Science and TechnologyAbstract Alternative splicing (AS) plays an essential role in development, differentiation and carcinogenesis. However, the mechanisms underlying splicing regulation during mouse embryo gastrulation remain unclear. Based on spatial-temporal transcriptome and epigenome data, we detected the dynamics of AS and revealed its regulatory mechanisms across primary germ layers during mouse gastrulation, spanning developmental stages from E6.5 to E7.5. Subsequently, the dynamic expression of splicing factors (SFs) during gastrulation was characterized, while the expression patterns and functions of germ layer-specific SFs were identified. The results indicate that AS and differential alternative splicing events (DASEs) exhibit dynamic changes and are significantly abundant during the late stage of gastrulation. Similarly, SFs demonstrate stage-specific expression, with elevated levels observed during the middle and late stages of gastrulation. Epigenetic signals associated with SFs and AS sites demonstrate significant enrichment and undergo dynamic changes throughout gastrulation. Overall, this study offers a systematic analysis of AS during mouse gastrulation, identifies primary germ layer-specific AS events, and characterizes the expression patterns of SFs and the associated epigenetic signals. These findings enhance the understanding of the mechanisms underlying the formation of the three germ layers during mammalian gastrulation, with a focus on pre-mRNA AS.https://doi.org/10.1038/s41598-025-96148-7Mouse gastrulationAlternative splicingSplicing factorsEpigenetic regulationEmbryonic development |
| spellingShingle | Wei Wang Yu Zhang Yuanyuan Zhai Wuritu Yang Yongqiang Xing Alternative splicing dynamics during gastrulation in mouse embryo Scientific Reports Mouse gastrulation Alternative splicing Splicing factors Epigenetic regulation Embryonic development |
| title | Alternative splicing dynamics during gastrulation in mouse embryo |
| title_full | Alternative splicing dynamics during gastrulation in mouse embryo |
| title_fullStr | Alternative splicing dynamics during gastrulation in mouse embryo |
| title_full_unstemmed | Alternative splicing dynamics during gastrulation in mouse embryo |
| title_short | Alternative splicing dynamics during gastrulation in mouse embryo |
| title_sort | alternative splicing dynamics during gastrulation in mouse embryo |
| topic | Mouse gastrulation Alternative splicing Splicing factors Epigenetic regulation Embryonic development |
| url | https://doi.org/10.1038/s41598-025-96148-7 |
| work_keys_str_mv | AT weiwang alternativesplicingdynamicsduringgastrulationinmouseembryo AT yuzhang alternativesplicingdynamicsduringgastrulationinmouseembryo AT yuanyuanzhai alternativesplicingdynamicsduringgastrulationinmouseembryo AT wurituyang alternativesplicingdynamicsduringgastrulationinmouseembryo AT yongqiangxing alternativesplicingdynamicsduringgastrulationinmouseembryo |