Unveiling the hemolymphatic miRNome composition of the schistosomiasis vector snail Biomphalaria glabrata
MiRNAs are single-stranded short noncoding sequences which display crucial roles on gene transcription regulation in many biological processes especially such as embryonic development, cell proliferation or apoptosis. Also, they are recognized for triggering the host’s internal defence mechanisms an...
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Elsevier
2025-01-01
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| Series: | Current Research in Parasitology and Vector-Borne Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667114X25000226 |
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| author | Sarah Dametto Benjamin Gourbal Cristian Chaparro Silvain Pinaud David Duval |
| author_facet | Sarah Dametto Benjamin Gourbal Cristian Chaparro Silvain Pinaud David Duval |
| author_sort | Sarah Dametto |
| collection | DOAJ |
| description | MiRNAs are single-stranded short noncoding sequences which display crucial roles on gene transcription regulation in many biological processes especially such as embryonic development, cell proliferation or apoptosis. Also, they are recognized for triggering the host’s internal defence mechanisms and immune cell responses thereby playing crucial role in host-parasite interactions. In the present study, a snap-shot of miRNAs, referred to as the miRNome, is described from the hemolymph, the main immune-related compartment of Biomphalaria glabrata snails, one of the intermediate hosts of the trematode parasite Schistosoma mansoni, the causative agent of schistosomiasis. A high throughput sequencing approach of small RNAs has revealed the presence of 63 miRNAs in the hemolymphatic compartment. Mollusc-specific miRNAs including bgl-miR-1985-5p and bgl-miR-1984-5p were identified, along with 25 novel miRNAs. Bioinformatic predictions, thanks to multiple software tools, helped us to identify more than 6000 potential miRNA target gene candidates. Among them is BgTEP1, a complement-like factor involved in parasite clearance. Interestingly, this factor appeared to be targeted by a newly identified miRNA, named bgl-miR-22707-5p. Our study underscores the inherent diversity of miRNAs in the hemolymph of B. glabrata and discusses their potential role in the regulation of the snail’s innate immune response. |
| format | Article |
| id | doaj-art-c947c16b58ac4226bd4e26b780fa4c03 |
| institution | OA Journals |
| issn | 2667-114X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Current Research in Parasitology and Vector-Borne Diseases |
| spelling | doaj-art-c947c16b58ac4226bd4e26b780fa4c032025-08-20T02:36:57ZengElsevierCurrent Research in Parasitology and Vector-Borne Diseases2667-114X2025-01-01710026210.1016/j.crpvbd.2025.100262Unveiling the hemolymphatic miRNome composition of the schistosomiasis vector snail Biomphalaria glabrataSarah Dametto0Benjamin Gourbal1Cristian Chaparro2Silvain Pinaud3David Duval4IHPE, Université de Perpignan Via Domitia, CNRS, Ifremer, Université de Montpellier, Perpignan, France; Corresponding author.IHPE, Université de Perpignan Via Domitia, CNRS, Ifremer, Université de Montpellier, Perpignan, FranceIHPE, Université de Perpignan Via Domitia, CNRS, Ifremer, Université de Montpellier, Perpignan, FranceMIVEGEC, Université de Montpellier, IRD, CNRS, Montpellier, FranceIHPE, Université de Perpignan Via Domitia, CNRS, Ifremer, Université de Montpellier, Perpignan, France; Corresponding author.MiRNAs are single-stranded short noncoding sequences which display crucial roles on gene transcription regulation in many biological processes especially such as embryonic development, cell proliferation or apoptosis. Also, they are recognized for triggering the host’s internal defence mechanisms and immune cell responses thereby playing crucial role in host-parasite interactions. In the present study, a snap-shot of miRNAs, referred to as the miRNome, is described from the hemolymph, the main immune-related compartment of Biomphalaria glabrata snails, one of the intermediate hosts of the trematode parasite Schistosoma mansoni, the causative agent of schistosomiasis. A high throughput sequencing approach of small RNAs has revealed the presence of 63 miRNAs in the hemolymphatic compartment. Mollusc-specific miRNAs including bgl-miR-1985-5p and bgl-miR-1984-5p were identified, along with 25 novel miRNAs. Bioinformatic predictions, thanks to multiple software tools, helped us to identify more than 6000 potential miRNA target gene candidates. Among them is BgTEP1, a complement-like factor involved in parasite clearance. Interestingly, this factor appeared to be targeted by a newly identified miRNA, named bgl-miR-22707-5p. Our study underscores the inherent diversity of miRNAs in the hemolymph of B. glabrata and discusses their potential role in the regulation of the snail’s innate immune response.http://www.sciencedirect.com/science/article/pii/S2667114X25000226Biomphalaria glabrataHemocytesmiRNASmall RNAsHost-parasite interaction |
| spellingShingle | Sarah Dametto Benjamin Gourbal Cristian Chaparro Silvain Pinaud David Duval Unveiling the hemolymphatic miRNome composition of the schistosomiasis vector snail Biomphalaria glabrata Current Research in Parasitology and Vector-Borne Diseases Biomphalaria glabrata Hemocytes miRNA Small RNAs Host-parasite interaction |
| title | Unveiling the hemolymphatic miRNome composition of the schistosomiasis vector snail Biomphalaria glabrata |
| title_full | Unveiling the hemolymphatic miRNome composition of the schistosomiasis vector snail Biomphalaria glabrata |
| title_fullStr | Unveiling the hemolymphatic miRNome composition of the schistosomiasis vector snail Biomphalaria glabrata |
| title_full_unstemmed | Unveiling the hemolymphatic miRNome composition of the schistosomiasis vector snail Biomphalaria glabrata |
| title_short | Unveiling the hemolymphatic miRNome composition of the schistosomiasis vector snail Biomphalaria glabrata |
| title_sort | unveiling the hemolymphatic mirnome composition of the schistosomiasis vector snail biomphalaria glabrata |
| topic | Biomphalaria glabrata Hemocytes miRNA Small RNAs Host-parasite interaction |
| url | http://www.sciencedirect.com/science/article/pii/S2667114X25000226 |
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