In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifolium
Introduction: In the classic treatment of Trichomonas vaginalis infection, although metronidazole has been used since the 1960s, there has been an increase in MTZ-resistant T. vaginalis strains and failure in the treatment of trichomoniasis causes serious concerns. Therefore, the present study aime...
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The Journal of Infection in Developing Countries
2019-03-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/10854 |
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| author | Aysegul A Gokmen Hüseyin Can Hüsniye Kayalar Bayram Pektaş Selçuk Kaya |
| author_facet | Aysegul A Gokmen Hüseyin Can Hüsniye Kayalar Bayram Pektaş Selçuk Kaya |
| author_sort | Aysegul A Gokmen |
| collection | DOAJ |
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Introduction: In the classic treatment of Trichomonas vaginalis infection, although metronidazole has been used since the 1960s, there has been an increase in MTZ-resistant T. vaginalis strains and failure in the treatment of trichomoniasis causes serious concerns. Therefore, the present study aimed to investigate the in vitro antitrichomonal activities of extracts (ethanol and total alkaloid) and pure compounds (chrysosplenetin, dictamnine, gamma-Fagarine, skimmianine) of H. myrtifolium against T. vaginalis.
Methodology: H. myrtifolium was collected from the town of Honaz in Denizli, located in the Aegean region of Turkey, and preparation of extracts and isolation and structure elucidation of pure compounds were performed. Later, different concentrations of extracts and pure compounds were incubated with T. vaginalis trophozoites isolated from Turkey, which are known to be sensitive to metronidazole.
Results: It was found that ethanol extract caused a more effective lysis on T. vaginalis trophozoites compared with total alkaloid extract (P < 0.05). No compounds except for furoquinoline alkaloid skimmianine prepared above 37.5 µg/mL were found to have any inhibitory effect on T. vaginalis trophozoites.
Conclusion: The ethanol extract of H. myrtifolium and skimmianine can be considered as potential candidates for antitrichomonal drug development.
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| format | Article |
| id | doaj-art-c9472e6ecbb34e0b93581b57f21ebf71 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2019-03-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-c9472e6ecbb34e0b93581b57f21ebf712025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-03-01130310.3855/jidc.10854In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifoliumAysegul A Gokmen0Hüseyin Can1Hüsniye Kayalar2Bayram Pektaş3Selçuk Kaya4Department of Microbiology, Faculty of Medicine, İzmir Katip Çelebi University, İzmir, TurkeyMolecular Biology Section, Department of Biology, Faculty of Science, Ege University, İzmir, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Ege University, İzmir, TurkeyDepartment of Microbiology, İzmir Atatürk Training and Research Hospital, Yeşilyurt, İzmir, TurkeyDepartment of Microbiology, Faculty of Medicine, İzmir Katip Çelebi University, İzmir, Turkey Introduction: In the classic treatment of Trichomonas vaginalis infection, although metronidazole has been used since the 1960s, there has been an increase in MTZ-resistant T. vaginalis strains and failure in the treatment of trichomoniasis causes serious concerns. Therefore, the present study aimed to investigate the in vitro antitrichomonal activities of extracts (ethanol and total alkaloid) and pure compounds (chrysosplenetin, dictamnine, gamma-Fagarine, skimmianine) of H. myrtifolium against T. vaginalis. Methodology: H. myrtifolium was collected from the town of Honaz in Denizli, located in the Aegean region of Turkey, and preparation of extracts and isolation and structure elucidation of pure compounds were performed. Later, different concentrations of extracts and pure compounds were incubated with T. vaginalis trophozoites isolated from Turkey, which are known to be sensitive to metronidazole. Results: It was found that ethanol extract caused a more effective lysis on T. vaginalis trophozoites compared with total alkaloid extract (P < 0.05). No compounds except for furoquinoline alkaloid skimmianine prepared above 37.5 µg/mL were found to have any inhibitory effect on T. vaginalis trophozoites. Conclusion: The ethanol extract of H. myrtifolium and skimmianine can be considered as potential candidates for antitrichomonal drug development. https://jidc.org/index.php/journal/article/view/10854Trichomanas vaginalisHaplophyllum myrtifoliumantitrichomonal activity |
| spellingShingle | Aysegul A Gokmen Hüseyin Can Hüsniye Kayalar Bayram Pektaş Selçuk Kaya In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifolium Journal of Infection in Developing Countries Trichomanas vaginalis Haplophyllum myrtifolium antitrichomonal activity |
| title | In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifolium |
| title_full | In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifolium |
| title_fullStr | In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifolium |
| title_full_unstemmed | In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifolium |
| title_short | In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifolium |
| title_sort | in vitro anti trichomonas vaginalis activity of haplophyllum myrtifolium |
| topic | Trichomanas vaginalis Haplophyllum myrtifolium antitrichomonal activity |
| url | https://jidc.org/index.php/journal/article/view/10854 |
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