The Effect of Nutrient Deprivation on Markers of Oxidative Stress, Inflammation, and Transcriptome in Normal and Type-2 Diabetic Human Skeletal Muscle Myoblasts

Conclusions: Myoblast viability and density were lower in the nutrient-deprived conditions and in the FBS compared to the HS serum. The biomarker of oxidative stress was lower in the serum concentration in a scaled effect, yet higher in HS. The biomarker of inflammation was higher in the HSMM cell l...

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Main Authors: Lael Ceriani, Daniel E. Newmire, Xavier F. Gonzales, Jean Sparks, Jose Guardiola, Felix O. Omoruyi
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:Journal of Nutrition and Metabolism
Online Access:http://dx.doi.org/10.1155/jnme/6661176
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author Lael Ceriani
Daniel E. Newmire
Xavier F. Gonzales
Jean Sparks
Jose Guardiola
Felix O. Omoruyi
author_facet Lael Ceriani
Daniel E. Newmire
Xavier F. Gonzales
Jean Sparks
Jose Guardiola
Felix O. Omoruyi
author_sort Lael Ceriani
collection DOAJ
description Conclusions: Myoblast viability and density were lower in the nutrient-deprived conditions and in the FBS compared to the HS serum. The biomarker of oxidative stress was lower in the serum concentration in a scaled effect, yet higher in HS. The biomarker of inflammation was higher in the HSMM cell line, shorter incubation time period, and in FBS. HS used as a media may supply nutrients and hormonal confounders that may support or stress myoblast growth.
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record_format Article
series Journal of Nutrition and Metabolism
spelling doaj-art-c94171dcfe284f6aaf5da0322addcb6a2025-08-20T02:20:40ZengWileyJournal of Nutrition and Metabolism2090-07322025-01-01202510.1155/jnme/6661176The Effect of Nutrient Deprivation on Markers of Oxidative Stress, Inflammation, and Transcriptome in Normal and Type-2 Diabetic Human Skeletal Muscle MyoblastsLael Ceriani0Daniel E. Newmire1Xavier F. Gonzales2Jean Sparks3Jose Guardiola4Felix O. Omoruyi5Department of Life SciencesSchool of Health Promotion and KinesiologyDepartment of Life SciencesDepartment of Health SciencesDepartment of Mathematics and StatisticsDepartment of Life SciencesConclusions: Myoblast viability and density were lower in the nutrient-deprived conditions and in the FBS compared to the HS serum. The biomarker of oxidative stress was lower in the serum concentration in a scaled effect, yet higher in HS. The biomarker of inflammation was higher in the HSMM cell line, shorter incubation time period, and in FBS. HS used as a media may supply nutrients and hormonal confounders that may support or stress myoblast growth.http://dx.doi.org/10.1155/jnme/6661176
spellingShingle Lael Ceriani
Daniel E. Newmire
Xavier F. Gonzales
Jean Sparks
Jose Guardiola
Felix O. Omoruyi
The Effect of Nutrient Deprivation on Markers of Oxidative Stress, Inflammation, and Transcriptome in Normal and Type-2 Diabetic Human Skeletal Muscle Myoblasts
Journal of Nutrition and Metabolism
title The Effect of Nutrient Deprivation on Markers of Oxidative Stress, Inflammation, and Transcriptome in Normal and Type-2 Diabetic Human Skeletal Muscle Myoblasts
title_full The Effect of Nutrient Deprivation on Markers of Oxidative Stress, Inflammation, and Transcriptome in Normal and Type-2 Diabetic Human Skeletal Muscle Myoblasts
title_fullStr The Effect of Nutrient Deprivation on Markers of Oxidative Stress, Inflammation, and Transcriptome in Normal and Type-2 Diabetic Human Skeletal Muscle Myoblasts
title_full_unstemmed The Effect of Nutrient Deprivation on Markers of Oxidative Stress, Inflammation, and Transcriptome in Normal and Type-2 Diabetic Human Skeletal Muscle Myoblasts
title_short The Effect of Nutrient Deprivation on Markers of Oxidative Stress, Inflammation, and Transcriptome in Normal and Type-2 Diabetic Human Skeletal Muscle Myoblasts
title_sort effect of nutrient deprivation on markers of oxidative stress inflammation and transcriptome in normal and type 2 diabetic human skeletal muscle myoblasts
url http://dx.doi.org/10.1155/jnme/6661176
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