Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17
Purpose. To investigate how multipotent retinal stem cells (RSCs) isolated from mice respond to the proinflammatory signaling molecules, IL-1β, IL-18, and IL-17A. Materials and Methods. RSCs were cultured in a specific culture medium and were treated with these cytokines. Cell viability was detected...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2015/369312 |
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| author | Shida Chen Defen Shen Nicholas A. Popp Alexander J. Ogilvy Jingsheng Tuo Mones Abu-Asab Ting Xie Chi-Chao Chan |
| author_facet | Shida Chen Defen Shen Nicholas A. Popp Alexander J. Ogilvy Jingsheng Tuo Mones Abu-Asab Ting Xie Chi-Chao Chan |
| author_sort | Shida Chen |
| collection | DOAJ |
| description | Purpose. To investigate how multipotent retinal stem cells (RSCs) isolated from mice respond to the proinflammatory signaling molecules, IL-1β, IL-18, and IL-17A. Materials and Methods. RSCs were cultured in a specific culture medium and were treated with these cytokines. Cell viability was detected by MTT assay; ultrastructure was evaluated by transmission electron microscopy; expression of IL-17rc and proapoptotic proteins was detected by immunocytochemistry and expression of Il-6 and Il-17a was detected by quantitative RT-PCR. As a comparison, primary mouse retinal pigment epithelium (RPE) cells were also treated with IL-1β, IL-18, or IL-17A and analyzed for the expression of Il-6 and Il-17rc. Results. Treatment with IL-1β, IL-18, or IL-17A decreased RSC viability in a dose-dependent fashion and led to damage in cellular ultrastructure including pyroptotic and/or necroptotic cells. IL-1β and IL-18 could induce proapoptotic protein expression. All treatments induced significantly higher expression of Il-6 and Il-17rc in both cells. However, neither IL-1β nor IL-18 could induce Il-17a expression in RSCs. Conclusions. IL-1β, IL-18, and IL-17A induce retinal cell death via pyroptosis/necroptosis and apoptosis. They also provoke proinflammatory responses in RSCs. Though IL-1β and IL-18 could not induce Il-17a expression in RSCs, they both increase Il-17rc expression, which may mediate the effect of Il-17a. |
| format | Article |
| id | doaj-art-c93a33bba0cb4fe3bae59b497d050ba0 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-c93a33bba0cb4fe3bae59b497d050ba02025-02-03T05:52:54ZengWileyJournal of Ophthalmology2090-004X2090-00582015-01-01201510.1155/2015/369312369312Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17Shida Chen0Defen Shen1Nicholas A. Popp2Alexander J. Ogilvy3Jingsheng Tuo4Mones Abu-Asab5Ting Xie6Chi-Chao Chan7Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USAHistology Core, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USALaboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USAHistology Core, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USAStowers Institute for Medical Research, Kansas City, MO 64110, USALaboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USAPurpose. To investigate how multipotent retinal stem cells (RSCs) isolated from mice respond to the proinflammatory signaling molecules, IL-1β, IL-18, and IL-17A. Materials and Methods. RSCs were cultured in a specific culture medium and were treated with these cytokines. Cell viability was detected by MTT assay; ultrastructure was evaluated by transmission electron microscopy; expression of IL-17rc and proapoptotic proteins was detected by immunocytochemistry and expression of Il-6 and Il-17a was detected by quantitative RT-PCR. As a comparison, primary mouse retinal pigment epithelium (RPE) cells were also treated with IL-1β, IL-18, or IL-17A and analyzed for the expression of Il-6 and Il-17rc. Results. Treatment with IL-1β, IL-18, or IL-17A decreased RSC viability in a dose-dependent fashion and led to damage in cellular ultrastructure including pyroptotic and/or necroptotic cells. IL-1β and IL-18 could induce proapoptotic protein expression. All treatments induced significantly higher expression of Il-6 and Il-17rc in both cells. However, neither IL-1β nor IL-18 could induce Il-17a expression in RSCs. Conclusions. IL-1β, IL-18, and IL-17A induce retinal cell death via pyroptosis/necroptosis and apoptosis. They also provoke proinflammatory responses in RSCs. Though IL-1β and IL-18 could not induce Il-17a expression in RSCs, they both increase Il-17rc expression, which may mediate the effect of Il-17a.http://dx.doi.org/10.1155/2015/369312 |
| spellingShingle | Shida Chen Defen Shen Nicholas A. Popp Alexander J. Ogilvy Jingsheng Tuo Mones Abu-Asab Ting Xie Chi-Chao Chan Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17 Journal of Ophthalmology |
| title | Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17 |
| title_full | Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17 |
| title_fullStr | Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17 |
| title_full_unstemmed | Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17 |
| title_short | Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17 |
| title_sort | responses of multipotent retinal stem cells to il 1β il 18 or il 17 |
| url | http://dx.doi.org/10.1155/2015/369312 |
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