Genome-wide characterization of the Eceriferum (CER) gene family in barley (Hordeum vulgare L.)
Abstract The Eceriferum (CER) gene family plays a crucial role in mitigating non-stomatal water loss and enhancing plant resilience to abiotic stresses, particularly drought and salinity. A comprehensive understanding of CER gene composition and expression dynamics is thereby, fundamental for develo...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-07145-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849238573176324096 |
|---|---|
| author | Bahman Panahi Rasmieh Hamid Feba Jacob |
| author_facet | Bahman Panahi Rasmieh Hamid Feba Jacob |
| author_sort | Bahman Panahi |
| collection | DOAJ |
| description | Abstract The Eceriferum (CER) gene family plays a crucial role in mitigating non-stomatal water loss and enhancing plant resilience to abiotic stresses, particularly drought and salinity. A comprehensive understanding of CER gene composition and expression dynamics is thereby, fundamental for developing stress-resilient crop varieties. In this study, we identified 12 CER genes in the barley (Hordeum vulgare L.) genome through an extensive genome-wide analysis. These genes were mapped across multiple chromosomes, and phylogenetic classification delineated them into distinct subgroups, reflecting their evolutionary divergence. Structural analyses, including exon-intron architecture, conserved motif composition, and protein domain organization, revealed a high degree of conservation within subfamilies. Collinearity analysis indicated a limited occurrence of gene duplication events, suggesting an evolutionary trajectory distinct from that observed in other species. Additionally, promoter region analysis uncovered diverse cis-regulatory elements associated with hormonal regulation and abiotic stress responses, providing insights into potential transcriptional regulatory mechanisms. Expression profiling across different stress conditions demonstrated stress-specific gene expression patterns and dynamic transcriptional responses to salinity and drought. Collectively, these findings offer valuable insights into the functional roles of CER genes in barley and identify promising candidates for genetic interventions aimed at improving stress tolerance in cereal crops. |
| format | Article |
| id | doaj-art-c934693c07be4c4faeb5121e725f3bd3 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-c934693c07be4c4faeb5121e725f3bd32025-08-20T04:01:34ZengNature PortfolioScientific Reports2045-23222025-07-0115111810.1038/s41598-025-07145-9Genome-wide characterization of the Eceriferum (CER) gene family in barley (Hordeum vulgare L.)Bahman Panahi0Rasmieh Hamid1Feba Jacob2Department of Genomics, Branch for Northwest & West region, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO)Department of Plant Breeding, Cotton Research Institute of Iran (CRII), Agricultural Research, Education and Extension Organization (AREEO)Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural UniversityAbstract The Eceriferum (CER) gene family plays a crucial role in mitigating non-stomatal water loss and enhancing plant resilience to abiotic stresses, particularly drought and salinity. A comprehensive understanding of CER gene composition and expression dynamics is thereby, fundamental for developing stress-resilient crop varieties. In this study, we identified 12 CER genes in the barley (Hordeum vulgare L.) genome through an extensive genome-wide analysis. These genes were mapped across multiple chromosomes, and phylogenetic classification delineated them into distinct subgroups, reflecting their evolutionary divergence. Structural analyses, including exon-intron architecture, conserved motif composition, and protein domain organization, revealed a high degree of conservation within subfamilies. Collinearity analysis indicated a limited occurrence of gene duplication events, suggesting an evolutionary trajectory distinct from that observed in other species. Additionally, promoter region analysis uncovered diverse cis-regulatory elements associated with hormonal regulation and abiotic stress responses, providing insights into potential transcriptional regulatory mechanisms. Expression profiling across different stress conditions demonstrated stress-specific gene expression patterns and dynamic transcriptional responses to salinity and drought. Collectively, these findings offer valuable insights into the functional roles of CER genes in barley and identify promising candidates for genetic interventions aimed at improving stress tolerance in cereal crops.https://doi.org/10.1038/s41598-025-07145-9BarleyEceriferum (CER) gene familyExpression analysisSalt stressDrought stress |
| spellingShingle | Bahman Panahi Rasmieh Hamid Feba Jacob Genome-wide characterization of the Eceriferum (CER) gene family in barley (Hordeum vulgare L.) Scientific Reports Barley Eceriferum (CER) gene family Expression analysis Salt stress Drought stress |
| title | Genome-wide characterization of the Eceriferum (CER) gene family in barley (Hordeum vulgare L.) |
| title_full | Genome-wide characterization of the Eceriferum (CER) gene family in barley (Hordeum vulgare L.) |
| title_fullStr | Genome-wide characterization of the Eceriferum (CER) gene family in barley (Hordeum vulgare L.) |
| title_full_unstemmed | Genome-wide characterization of the Eceriferum (CER) gene family in barley (Hordeum vulgare L.) |
| title_short | Genome-wide characterization of the Eceriferum (CER) gene family in barley (Hordeum vulgare L.) |
| title_sort | genome wide characterization of the eceriferum cer gene family in barley hordeum vulgare l |
| topic | Barley Eceriferum (CER) gene family Expression analysis Salt stress Drought stress |
| url | https://doi.org/10.1038/s41598-025-07145-9 |
| work_keys_str_mv | AT bahmanpanahi genomewidecharacterizationoftheeceriferumcergenefamilyinbarleyhordeumvulgarel AT rasmiehhamid genomewidecharacterizationoftheeceriferumcergenefamilyinbarleyhordeumvulgarel AT febajacob genomewidecharacterizationoftheeceriferumcergenefamilyinbarleyhordeumvulgarel |