Intra- and Interspecies Conjugal Transfer of Plasmids in Gram-Negative Bacteria
<b>Background/Objectives:</b> Plasmid-mediated resistance is a significant mechanism that contributes to the gradual decrease in the efficacy of antibiotics from various classes, including carbapenems. The aim of this study is to investigate the frequency of transfer of carbapenemase-enc...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/13/1/238 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589008145743872 |
|---|---|
| author | Julia R. Savelieva Daria A. Kondratieva Maria V. Golikova |
| author_facet | Julia R. Savelieva Daria A. Kondratieva Maria V. Golikova |
| author_sort | Julia R. Savelieva |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Plasmid-mediated resistance is a significant mechanism that contributes to the gradual decrease in the efficacy of antibiotics from various classes, including carbapenems. The aim of this study is to investigate the frequency of transfer of carbapenemase-encoding plasmids from <i>K. pneumoniae</i> to <i>E. coli</i> and <i>P. aeruginosa</i>. <b>Methods:</b> Matings were performed on agar with subsequent isolation of transconjugant, recipient, and donor colonies. The frequency of conjugation (CF) and minimum inhibitory concentrations (MICs) of meropenem were determined for the PCR-confirmed transconjugants. A pharmacodynamic study was conducted using a hollow-fiber infection model on <i>E. coli</i> transconjugant in order to evaluate its viability in the presence of therapeutic concentrations of meropenem. <b>Results:</b> CF for <i>K. pneumoniae</i>-<i>K. pneumoniae</i> was similar to that for <i>K. pneumoniae</i>-<i>E. coli</i> and was higher the higher was meropenem MIC of the <i>K. pneumoniae</i> donor. The meropenem MICs for <i>K. pneumoniae</i> and <i>E. coli</i> transconjugants were higher (0.25–4 μg/mL) compared to recipients (0.03–0.06 μg/mL). <i>P. aeruginosa</i> did not acquire plasmids from <i>K. pneumoniae</i>. In pharmacodynamic experiments, an <i>E. coli</i> transconjugant with MIC of 2 mg/L within the “susceptibility range”, failed to respond to meropenem treatment. <b>Conclusions:</b> The frequency of conjugation between <i>K. pneumoniae</i> and <i>E. coli</i> falls within a similar range. A higher permissiveness of <i>K. pneumoniae</i> for plasmids from <i>K. pneumoniae</i>, i.e., within the same species, was observed. Conjugation did not occur between <i>K. pneumoniae</i> and <i>P. aeruginosa</i>. The transconjugants with meropenem MICs with borderline susceptibility may pose a potential threat to the efficacy of meropenem. |
| format | Article |
| id | doaj-art-c931d963aca24f10b284cee43d9baa7f |
| institution | Kabale University |
| issn | 2227-9059 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj-art-c931d963aca24f10b284cee43d9baa7f2025-01-24T13:24:30ZengMDPI AGBiomedicines2227-90592025-01-0113123810.3390/biomedicines13010238Intra- and Interspecies Conjugal Transfer of Plasmids in Gram-Negative BacteriaJulia R. Savelieva0Daria A. Kondratieva1Maria V. Golikova2Department of Pharmacokinetics & Pharmacodynamics, Gause Institute of New Antibiotics, 11 Bolshaya Pirogovskaya Street, 119021 Moscow, RussiaDepartment of Pharmacokinetics & Pharmacodynamics, Gause Institute of New Antibiotics, 11 Bolshaya Pirogovskaya Street, 119021 Moscow, RussiaDepartment of Pharmacokinetics & Pharmacodynamics, Gause Institute of New Antibiotics, 11 Bolshaya Pirogovskaya Street, 119021 Moscow, Russia<b>Background/Objectives:</b> Plasmid-mediated resistance is a significant mechanism that contributes to the gradual decrease in the efficacy of antibiotics from various classes, including carbapenems. The aim of this study is to investigate the frequency of transfer of carbapenemase-encoding plasmids from <i>K. pneumoniae</i> to <i>E. coli</i> and <i>P. aeruginosa</i>. <b>Methods:</b> Matings were performed on agar with subsequent isolation of transconjugant, recipient, and donor colonies. The frequency of conjugation (CF) and minimum inhibitory concentrations (MICs) of meropenem were determined for the PCR-confirmed transconjugants. A pharmacodynamic study was conducted using a hollow-fiber infection model on <i>E. coli</i> transconjugant in order to evaluate its viability in the presence of therapeutic concentrations of meropenem. <b>Results:</b> CF for <i>K. pneumoniae</i>-<i>K. pneumoniae</i> was similar to that for <i>K. pneumoniae</i>-<i>E. coli</i> and was higher the higher was meropenem MIC of the <i>K. pneumoniae</i> donor. The meropenem MICs for <i>K. pneumoniae</i> and <i>E. coli</i> transconjugants were higher (0.25–4 μg/mL) compared to recipients (0.03–0.06 μg/mL). <i>P. aeruginosa</i> did not acquire plasmids from <i>K. pneumoniae</i>. In pharmacodynamic experiments, an <i>E. coli</i> transconjugant with MIC of 2 mg/L within the “susceptibility range”, failed to respond to meropenem treatment. <b>Conclusions:</b> The frequency of conjugation between <i>K. pneumoniae</i> and <i>E. coli</i> falls within a similar range. A higher permissiveness of <i>K. pneumoniae</i> for plasmids from <i>K. pneumoniae</i>, i.e., within the same species, was observed. Conjugation did not occur between <i>K. pneumoniae</i> and <i>P. aeruginosa</i>. The transconjugants with meropenem MICs with borderline susceptibility may pose a potential threat to the efficacy of meropenem.https://www.mdpi.com/2227-9059/13/1/238conjugation<i>Klebsiella pneumoniae</i><i>Escherichia coli</i><i>Pseudomonas aeruginosa</i>meropenemcarbapenemases |
| spellingShingle | Julia R. Savelieva Daria A. Kondratieva Maria V. Golikova Intra- and Interspecies Conjugal Transfer of Plasmids in Gram-Negative Bacteria Biomedicines conjugation <i>Klebsiella pneumoniae</i> <i>Escherichia coli</i> <i>Pseudomonas aeruginosa</i> meropenem carbapenemases |
| title | Intra- and Interspecies Conjugal Transfer of Plasmids in Gram-Negative Bacteria |
| title_full | Intra- and Interspecies Conjugal Transfer of Plasmids in Gram-Negative Bacteria |
| title_fullStr | Intra- and Interspecies Conjugal Transfer of Plasmids in Gram-Negative Bacteria |
| title_full_unstemmed | Intra- and Interspecies Conjugal Transfer of Plasmids in Gram-Negative Bacteria |
| title_short | Intra- and Interspecies Conjugal Transfer of Plasmids in Gram-Negative Bacteria |
| title_sort | intra and interspecies conjugal transfer of plasmids in gram negative bacteria |
| topic | conjugation <i>Klebsiella pneumoniae</i> <i>Escherichia coli</i> <i>Pseudomonas aeruginosa</i> meropenem carbapenemases |
| url | https://www.mdpi.com/2227-9059/13/1/238 |
| work_keys_str_mv | AT juliarsavelieva intraandinterspeciesconjugaltransferofplasmidsingramnegativebacteria AT dariaakondratieva intraandinterspeciesconjugaltransferofplasmidsingramnegativebacteria AT mariavgolikova intraandinterspeciesconjugaltransferofplasmidsingramnegativebacteria |