In Vitro Analysis of Healing Properties of Platelet-Rich Fibrin (PRF) vs. Collagen Membranes in Guided Tissue Regeneration
Aim: The present study sought to assess, using growth factor release and tissue integration, the in vitro healing characteristics of collagen membranes and platelet-rich fibrin (PRF) in guided tissue regeneration (GTR). Materials and Methods: The control was commercially available collagen membranes...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-05-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
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| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_491_25 |
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| author | B Saravana Kumar Ushma H. Prajapati Neelam Das K. Vel Latha Vishal S. Shah Arshiya F. Mohammadi |
| author_facet | B Saravana Kumar Ushma H. Prajapati Neelam Das K. Vel Latha Vishal S. Shah Arshiya F. Mohammadi |
| author_sort | B Saravana Kumar |
| collection | DOAJ |
| description | Aim:
The present study sought to assess, using growth factor release and tissue integration, the in vitro healing characteristics of collagen membranes and platelet-rich fibrin (PRF) in guided tissue regeneration (GTR).
Materials and Methods:
The control was commercially available collagen membranes; PRF membranes were produced using a normal centrifugation technique. Scanning electron microscopy (SEM) was used to examine structural features. Key growth factors—including transforming growth factor-beta 1 (TGF-β1), platelet-derived growth factor (PDGF-BB), and vascular endothelial growth factor (VEGF)—were assessed using enzyme-linked immunosorbent assays (ELISA). Using scratch tests, we assessed wound healing capacity and fibroblast migration. The student’s t-test was used for statistical analysis; P < 0.001 was considered to be significant.
Results:
While collagen membranes showed a consistent porosity shape, PRF showed a rich fibrin network, including embedded platelets and leukocytes. At 14 days, PRF showed much more cell growth (P < 0.001). Growth factor analysis showed that PRF generated more TGF-β1, PDGF-BB, and VEGF with consistent release (P < 0.001). PRF shows greater healing properties than collagen membranes with enhanced cellular proliferation, movement, and release of growth factors.
Conclusion:
These results suggest that PRF might be a more effective biomaterial for use in guided tissue regeneration. |
| format | Article |
| id | doaj-art-c92fa46bd10a45c2a93441007465adf1 |
| institution | DOAJ |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-c92fa46bd10a45c2a93441007465adf12025-08-20T03:07:47ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062025-05-0117Suppl 1S519S52110.4103/jpbs.jpbs_491_25In Vitro Analysis of Healing Properties of Platelet-Rich Fibrin (PRF) vs. Collagen Membranes in Guided Tissue RegenerationB Saravana KumarUshma H. PrajapatiNeelam DasK. Vel LathaVishal S. ShahArshiya F. MohammadiAim: The present study sought to assess, using growth factor release and tissue integration, the in vitro healing characteristics of collagen membranes and platelet-rich fibrin (PRF) in guided tissue regeneration (GTR). Materials and Methods: The control was commercially available collagen membranes; PRF membranes were produced using a normal centrifugation technique. Scanning electron microscopy (SEM) was used to examine structural features. Key growth factors—including transforming growth factor-beta 1 (TGF-β1), platelet-derived growth factor (PDGF-BB), and vascular endothelial growth factor (VEGF)—were assessed using enzyme-linked immunosorbent assays (ELISA). Using scratch tests, we assessed wound healing capacity and fibroblast migration. The student’s t-test was used for statistical analysis; P < 0.001 was considered to be significant. Results: While collagen membranes showed a consistent porosity shape, PRF showed a rich fibrin network, including embedded platelets and leukocytes. At 14 days, PRF showed much more cell growth (P < 0.001). Growth factor analysis showed that PRF generated more TGF-β1, PDGF-BB, and VEGF with consistent release (P < 0.001). PRF shows greater healing properties than collagen membranes with enhanced cellular proliferation, movement, and release of growth factors. Conclusion: These results suggest that PRF might be a more effective biomaterial for use in guided tissue regeneration.https://journals.lww.com/10.4103/jpbs.jpbs_491_25collagen membraneplatelet derivativesregeneration |
| spellingShingle | B Saravana Kumar Ushma H. Prajapati Neelam Das K. Vel Latha Vishal S. Shah Arshiya F. Mohammadi In Vitro Analysis of Healing Properties of Platelet-Rich Fibrin (PRF) vs. Collagen Membranes in Guided Tissue Regeneration Journal of Pharmacy and Bioallied Sciences collagen membrane platelet derivatives regeneration |
| title | In Vitro Analysis of Healing Properties of Platelet-Rich Fibrin (PRF) vs. Collagen Membranes in Guided Tissue Regeneration |
| title_full | In Vitro Analysis of Healing Properties of Platelet-Rich Fibrin (PRF) vs. Collagen Membranes in Guided Tissue Regeneration |
| title_fullStr | In Vitro Analysis of Healing Properties of Platelet-Rich Fibrin (PRF) vs. Collagen Membranes in Guided Tissue Regeneration |
| title_full_unstemmed | In Vitro Analysis of Healing Properties of Platelet-Rich Fibrin (PRF) vs. Collagen Membranes in Guided Tissue Regeneration |
| title_short | In Vitro Analysis of Healing Properties of Platelet-Rich Fibrin (PRF) vs. Collagen Membranes in Guided Tissue Regeneration |
| title_sort | in vitro analysis of healing properties of platelet rich fibrin prf vs collagen membranes in guided tissue regeneration |
| topic | collagen membrane platelet derivatives regeneration |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_491_25 |
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