Use of Manganese in RT-PCR Eliminates PCR Artifacts Resulting from DNase I Digestion

Use of Manganese in RT-PCR Eliminates PCR Artifacts Resulting from DNase I Digestion

The precise quantification of rare mRNA copies from intronless genes by reverse transcription polymerase chain reaction (RT-PCR) requires the complete removal of genomic DNA because discrimination of cDNA and DNA amplification products by differing sizes of PCR products is not possible. Elimination...

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Bibliographic Details
Main Authors: P. Bauer, A. Rolfs, V. Regitz-Zagrosek, A. Hildebrandt, E. Fleck
Format: Article
Language:English
Published: Taylor & Francis Group 1997-06-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/97226st05
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