Acetone–Ether–Water Mouse Model of Persistent Itch Fully Resolves Without Latent Pruritic or Cross-Modality Priming
Hyperalgesic priming is a model of the transition from acute to chronic pain. Whether a similar mechanism exists for “pruritic priming” of itch is unknown. Here, we tested the hypothesis that itchy skin in a commonly used mouse model of dry skin pruritus develops latent sensitization after resolutio...
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MDPI AG
2025-02-01
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| Series: | Dermatopathology |
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| Online Access: | https://www.mdpi.com/2296-3529/12/1/5 |
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| author | Zachary K. Ford Adam J. Kirry Steve Davidson |
| author_facet | Zachary K. Ford Adam J. Kirry Steve Davidson |
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| description | Hyperalgesic priming is a model of the transition from acute to chronic pain. Whether a similar mechanism exists for “pruritic priming” of itch is unknown. Here, we tested the hypothesis that itchy skin in a commonly used mouse model of dry skin pruritus develops latent sensitization after resolution. Acetone–ether–water (AEW) treatment induced a dry and itchy skin condition in the mouse cheek that elicited site-directed scratching behavior. After cessation of treatment and the complete resolution of AEW-induced scratching, histaminergic and non-histaminergic pruritogens were administered to the cheek to test for altered site-directed scratching and wiping behavior. Each pruritogen was also tested following the resolution of carrageenan-induced nociceptor hypersensitivity to test for cross-modality priming. Peak AEW-induced scratching occurred 24 h after the final day of treatment, and 5 days were required for scratching levels to return to baseline. Likewise, epidermal thickening was the greatest on the final treatment day and completely returned to baseline after 5 days. After the resolution of itchy cheek skin, acute histamine- and non-histamine-evoked scratching and wiping behaviors were unchanged, nor were scratching and wiping behaviors to acute pruritogens altered after the resolution of carrageenan-induced hypersensitivity. The results indicate that persistent itch due to dry skin likely resolves completely, without producing a latent primed response to subsequent pruritic stimuli. We conclude that the mechanisms regulating hyperalgesic priming are likely distinct from pruritic signaling in the dry and itchy skin model. |
| format | Article |
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| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-c91aa1a643cb4f6a9e7f1364596bd40e2025-08-20T02:11:16ZengMDPI AGDermatopathology2296-35292025-02-01121510.3390/dermatopathology12010005Acetone–Ether–Water Mouse Model of Persistent Itch Fully Resolves Without Latent Pruritic or Cross-Modality PrimingZachary K. Ford0Adam J. Kirry1Steve Davidson2Neuroscience Graduate Program, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USADepartment of Anesthesiology, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USANeuroscience Graduate Program, College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USAHyperalgesic priming is a model of the transition from acute to chronic pain. Whether a similar mechanism exists for “pruritic priming” of itch is unknown. Here, we tested the hypothesis that itchy skin in a commonly used mouse model of dry skin pruritus develops latent sensitization after resolution. Acetone–ether–water (AEW) treatment induced a dry and itchy skin condition in the mouse cheek that elicited site-directed scratching behavior. After cessation of treatment and the complete resolution of AEW-induced scratching, histaminergic and non-histaminergic pruritogens were administered to the cheek to test for altered site-directed scratching and wiping behavior. Each pruritogen was also tested following the resolution of carrageenan-induced nociceptor hypersensitivity to test for cross-modality priming. Peak AEW-induced scratching occurred 24 h after the final day of treatment, and 5 days were required for scratching levels to return to baseline. Likewise, epidermal thickening was the greatest on the final treatment day and completely returned to baseline after 5 days. After the resolution of itchy cheek skin, acute histamine- and non-histamine-evoked scratching and wiping behaviors were unchanged, nor were scratching and wiping behaviors to acute pruritogens altered after the resolution of carrageenan-induced hypersensitivity. The results indicate that persistent itch due to dry skin likely resolves completely, without producing a latent primed response to subsequent pruritic stimuli. We conclude that the mechanisms regulating hyperalgesic priming are likely distinct from pruritic signaling in the dry and itchy skin model.https://www.mdpi.com/2296-3529/12/1/5itchdry skinprimingpruritisAEWepidermis |
| spellingShingle | Zachary K. Ford Adam J. Kirry Steve Davidson Acetone–Ether–Water Mouse Model of Persistent Itch Fully Resolves Without Latent Pruritic or Cross-Modality Priming Dermatopathology itch dry skin priming pruritis AEW epidermis |
| title | Acetone–Ether–Water Mouse Model of Persistent Itch Fully Resolves Without Latent Pruritic or Cross-Modality Priming |
| title_full | Acetone–Ether–Water Mouse Model of Persistent Itch Fully Resolves Without Latent Pruritic or Cross-Modality Priming |
| title_fullStr | Acetone–Ether–Water Mouse Model of Persistent Itch Fully Resolves Without Latent Pruritic or Cross-Modality Priming |
| title_full_unstemmed | Acetone–Ether–Water Mouse Model of Persistent Itch Fully Resolves Without Latent Pruritic or Cross-Modality Priming |
| title_short | Acetone–Ether–Water Mouse Model of Persistent Itch Fully Resolves Without Latent Pruritic or Cross-Modality Priming |
| title_sort | acetone ether water mouse model of persistent itch fully resolves without latent pruritic or cross modality priming |
| topic | itch dry skin priming pruritis AEW epidermis |
| url | https://www.mdpi.com/2296-3529/12/1/5 |
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