Preharvest Application of Exogenous 2,4-Epibrassinolide and Melatonin Enhances the Maturity and Flue-Cured Quality of Tobacco Leaves
Tobacco (<i>Nicotiana tabacum</i>) is a globally cultivated crop, with its quality closely associated with the color and chemical composition of cured tobacco leaves. In this experiment, the effects of spraying exogenous 2, 4-epibrassinolide (EBR) and melatonin (MT) on the development of...
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2024-11-01
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| author | Kesu Wei Jiayi Tang Lei Yang Shaopeng Chen Zhijun Cheng Yijun Yang Chen Xu Shengjiang Wu Yuhang Zhao Hongmei Di Ling Li Dongyang Sun Jianwei Li Bo Sun |
| author_facet | Kesu Wei Jiayi Tang Lei Yang Shaopeng Chen Zhijun Cheng Yijun Yang Chen Xu Shengjiang Wu Yuhang Zhao Hongmei Di Ling Li Dongyang Sun Jianwei Li Bo Sun |
| author_sort | Kesu Wei |
| collection | DOAJ |
| description | Tobacco (<i>Nicotiana tabacum</i>) is a globally cultivated crop, with its quality closely associated with the color and chemical composition of cured tobacco leaves. In this experiment, the effects of spraying exogenous 2, 4-epibrassinolide (EBR) and melatonin (MT) on the development of tobacco leaves at maturity stage and the quality after curing were investigated. Both EBR and MT treatments significantly enhanced the appearance quality of tobacco leaves at the stem-drying stage. Following preharvest applications, the sugar-to-alkali ratio and potassium content increased, while the contents of starch, total alkaloids, and proteins decreased. The levels of conventional chemical components were improved, enhancing the overall coordination of the tobacco. Transcriptome analysis revealed that EBR treatment down-regulated the chlorophyll biosynthetic genes <i>hemA</i>, <i>MgPEC</i>, and <i>ChlD</i>, while up-regulating the chlorophyll degradation genes <i>CHL2</i>, <i>SGR</i>, and <i>PAOs</i>. Similarly, MT treatment down-regulated the chlorophyll biosynthetic genes <i>FC2</i> and <i>MgPEC</i> and up-regulated the degradation genes <i>CHL2</i> and <i>SGR</i>, thus promoting chlorophyll degradation. Furthermore, in the downstream carotenoid biosynthetic pathway, both EBR and MT treatments regulated abscisic acid-related genes, with <i>NCEDs</i> being up-regulated and <i>CYP707A1s</i> down-regulated, thereby promoting the leaf ripening. Metabolomics analysis indicated that EBR treatment primarily regulated alkaloids, terpenoids, and flavonoids, while MT treatment mainly affected flavonoids. Both treatments also reduced the accumulation of the harmful substance aristolochic acid B. Comprehensive evaluations of appearance quality, physiological parameters, transcriptome, and metabolomics analyses demonstrated that exogenous spraying of EBR and MT treatments improved the maturity and quality of cured tobacco leaves, with EBR treatment exhibiting a greater effect than MT treatment. |
| format | Article |
| id | doaj-art-c919dae385e54477a7ef60cf8e21348a |
| institution | OA Journals |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-11-01 |
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| series | Plants |
| spelling | doaj-art-c919dae385e54477a7ef60cf8e21348a2025-08-20T02:38:50ZengMDPI AGPlants2223-77472024-11-011323326610.3390/plants13233266Preharvest Application of Exogenous 2,4-Epibrassinolide and Melatonin Enhances the Maturity and Flue-Cured Quality of Tobacco LeavesKesu Wei0Jiayi Tang1Lei Yang2Shaopeng Chen3Zhijun Cheng4Yijun Yang5Chen Xu6Shengjiang Wu7Yuhang Zhao8Hongmei Di9Ling Li10Dongyang Sun11Jianwei Li12Bo Sun13Guizhou Academy of Tobacco Science, Guizhou Provincial Academician Workstation of Microbiology and Health, Guiyang 550081, ChinaGuizhou Academy of Tobacco Science, Guizhou Provincial Academician Workstation of Microbiology and Health, Guiyang 550081, ChinaGuizhou Academy of Tobacco Science, Guizhou Provincial Academician Workstation of Microbiology and Health, Guiyang 550081, ChinaChongqing Tobacco Science Institute, Chongqing 409199, ChinaGuizhou Academy of Tobacco Science, Guizhou Provincial Academician Workstation of Microbiology and Health, Guiyang 550081, ChinaGuizhou Academy of Tobacco Science, Guizhou Provincial Academician Workstation of Microbiology and Health, Guiyang 550081, ChinaChongqing Tobacco Science Institute, Chongqing 409199, ChinaGuizhou Academy of Tobacco Science, Guizhou Provincial Academician Workstation of Microbiology and Health, Guiyang 550081, ChinaGuizhou Academy of Tobacco Science, Guizhou Provincial Academician Workstation of Microbiology and Health, Guiyang 550081, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaGuizhou Academy of Tobacco Science, Guizhou Provincial Academician Workstation of Microbiology and Health, Guiyang 550081, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaTobacco (<i>Nicotiana tabacum</i>) is a globally cultivated crop, with its quality closely associated with the color and chemical composition of cured tobacco leaves. In this experiment, the effects of spraying exogenous 2, 4-epibrassinolide (EBR) and melatonin (MT) on the development of tobacco leaves at maturity stage and the quality after curing were investigated. Both EBR and MT treatments significantly enhanced the appearance quality of tobacco leaves at the stem-drying stage. Following preharvest applications, the sugar-to-alkali ratio and potassium content increased, while the contents of starch, total alkaloids, and proteins decreased. The levels of conventional chemical components were improved, enhancing the overall coordination of the tobacco. Transcriptome analysis revealed that EBR treatment down-regulated the chlorophyll biosynthetic genes <i>hemA</i>, <i>MgPEC</i>, and <i>ChlD</i>, while up-regulating the chlorophyll degradation genes <i>CHL2</i>, <i>SGR</i>, and <i>PAOs</i>. Similarly, MT treatment down-regulated the chlorophyll biosynthetic genes <i>FC2</i> and <i>MgPEC</i> and up-regulated the degradation genes <i>CHL2</i> and <i>SGR</i>, thus promoting chlorophyll degradation. Furthermore, in the downstream carotenoid biosynthetic pathway, both EBR and MT treatments regulated abscisic acid-related genes, with <i>NCEDs</i> being up-regulated and <i>CYP707A1s</i> down-regulated, thereby promoting the leaf ripening. Metabolomics analysis indicated that EBR treatment primarily regulated alkaloids, terpenoids, and flavonoids, while MT treatment mainly affected flavonoids. Both treatments also reduced the accumulation of the harmful substance aristolochic acid B. Comprehensive evaluations of appearance quality, physiological parameters, transcriptome, and metabolomics analyses demonstrated that exogenous spraying of EBR and MT treatments improved the maturity and quality of cured tobacco leaves, with EBR treatment exhibiting a greater effect than MT treatment.https://www.mdpi.com/2223-7747/13/23/3266<i>Nicotiana tabacum</i>2,4-epibrassinolidemelatoninflue-cured qualitytranscriptomemetabolome |
| spellingShingle | Kesu Wei Jiayi Tang Lei Yang Shaopeng Chen Zhijun Cheng Yijun Yang Chen Xu Shengjiang Wu Yuhang Zhao Hongmei Di Ling Li Dongyang Sun Jianwei Li Bo Sun Preharvest Application of Exogenous 2,4-Epibrassinolide and Melatonin Enhances the Maturity and Flue-Cured Quality of Tobacco Leaves Plants <i>Nicotiana tabacum</i> 2,4-epibrassinolide melatonin flue-cured quality transcriptome metabolome |
| title | Preharvest Application of Exogenous 2,4-Epibrassinolide and Melatonin Enhances the Maturity and Flue-Cured Quality of Tobacco Leaves |
| title_full | Preharvest Application of Exogenous 2,4-Epibrassinolide and Melatonin Enhances the Maturity and Flue-Cured Quality of Tobacco Leaves |
| title_fullStr | Preharvest Application of Exogenous 2,4-Epibrassinolide and Melatonin Enhances the Maturity and Flue-Cured Quality of Tobacco Leaves |
| title_full_unstemmed | Preharvest Application of Exogenous 2,4-Epibrassinolide and Melatonin Enhances the Maturity and Flue-Cured Quality of Tobacco Leaves |
| title_short | Preharvest Application of Exogenous 2,4-Epibrassinolide and Melatonin Enhances the Maturity and Flue-Cured Quality of Tobacco Leaves |
| title_sort | preharvest application of exogenous 2 4 epibrassinolide and melatonin enhances the maturity and flue cured quality of tobacco leaves |
| topic | <i>Nicotiana tabacum</i> 2,4-epibrassinolide melatonin flue-cured quality transcriptome metabolome |
| url | https://www.mdpi.com/2223-7747/13/23/3266 |
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