The targeted delivery of anti-metastasis oligonucleotide in breast cancer cells by the nanoliposomes conjugated with RGD ligand
To decrease the ETV4 gene, which is important in metastasis, the nanoliposome containing ETV4 antisense oligonucleotide conjugated with Arginylglycylaspartic acid (RGD) ligand was used in the study. The nanoliposome containing ETV4 antisense oligonucleotide conjugated with RGD ligand was synthesized...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Advances in Cancer Biology - Metastasis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667394025000097 |
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| author | Farahanaz Kavian Manesh Ali Jebali Flora Forouzesh |
| author_facet | Farahanaz Kavian Manesh Ali Jebali Flora Forouzesh |
| author_sort | Farahanaz Kavian Manesh |
| collection | DOAJ |
| description | To decrease the ETV4 gene, which is important in metastasis, the nanoliposome containing ETV4 antisense oligonucleotide conjugated with Arginylglycylaspartic acid (RGD) ligand was used in the study. The nanoliposome containing ETV4 antisense oligonucleotide conjugated with RGD ligand was synthesized and characterized by AFM, DLS, and FTIR. Then, MDA-MB-231, MCF-7, and MCF-10A cell lines were treated with different concentrations of nanoliposomes containing antisense ETV4 oligonucleotide conjugated with RGD, nanoliposomes without antisense ETV4 oligonucleotide, and antisense ETV4 oligonucleotide for 24 h. Finally, an MTT assay was used to evaluate cell viability, Real-time PCR was employed to assess the ETV4 mRNA expression, and western blotting was applied to evaluate the expression of ETV4 protein. Here, the characterization data revealed that the nanoliposome containing antisense ETV4 oligonucleotide conjugated with RGD had a spherical shape with a size range of 50–120 nm and zeta potential of −18mV to +7 mV. This study showed that although nanoliposomes containing antisense ETV4 oligonucleotide conjugated with RGD could effectively decrease the cell viability of MDA-MB-231 and MCF-7 cells, they could not dcrease cell viability of MCF-10A, indicating the effect of RGD ligand. Also, this novel carrier could decrease both mRNA and protein of the ETV4 gene in a dose dependent manner. |
| format | Article |
| id | doaj-art-c91412cb4fb249dc9f9e6eb37e73dbab |
| institution | OA Journals |
| issn | 2667-3940 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Cancer Biology - Metastasis |
| spelling | doaj-art-c91412cb4fb249dc9f9e6eb37e73dbab2025-08-20T02:06:00ZengElsevierAdvances in Cancer Biology - Metastasis2667-39402025-06-011410014010.1016/j.adcanc.2025.100140The targeted delivery of anti-metastasis oligonucleotide in breast cancer cells by the nanoliposomes conjugated with RGD ligandFarahanaz Kavian Manesh0Ali Jebali1Flora Forouzesh2Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, IranDepartment of Nanotechnology, Faculty of Advanced Science Taband Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran; Corresponding author. Department of Nanotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran; Corresponding author.To decrease the ETV4 gene, which is important in metastasis, the nanoliposome containing ETV4 antisense oligonucleotide conjugated with Arginylglycylaspartic acid (RGD) ligand was used in the study. The nanoliposome containing ETV4 antisense oligonucleotide conjugated with RGD ligand was synthesized and characterized by AFM, DLS, and FTIR. Then, MDA-MB-231, MCF-7, and MCF-10A cell lines were treated with different concentrations of nanoliposomes containing antisense ETV4 oligonucleotide conjugated with RGD, nanoliposomes without antisense ETV4 oligonucleotide, and antisense ETV4 oligonucleotide for 24 h. Finally, an MTT assay was used to evaluate cell viability, Real-time PCR was employed to assess the ETV4 mRNA expression, and western blotting was applied to evaluate the expression of ETV4 protein. Here, the characterization data revealed that the nanoliposome containing antisense ETV4 oligonucleotide conjugated with RGD had a spherical shape with a size range of 50–120 nm and zeta potential of −18mV to +7 mV. This study showed that although nanoliposomes containing antisense ETV4 oligonucleotide conjugated with RGD could effectively decrease the cell viability of MDA-MB-231 and MCF-7 cells, they could not dcrease cell viability of MCF-10A, indicating the effect of RGD ligand. Also, this novel carrier could decrease both mRNA and protein of the ETV4 gene in a dose dependent manner.http://www.sciencedirect.com/science/article/pii/S2667394025000097Breast cancerETV4 geneNanoliposomeAntisense oligonucleotideRGD ligand |
| spellingShingle | Farahanaz Kavian Manesh Ali Jebali Flora Forouzesh The targeted delivery of anti-metastasis oligonucleotide in breast cancer cells by the nanoliposomes conjugated with RGD ligand Advances in Cancer Biology - Metastasis Breast cancer ETV4 gene Nanoliposome Antisense oligonucleotide RGD ligand |
| title | The targeted delivery of anti-metastasis oligonucleotide in breast cancer cells by the nanoliposomes conjugated with RGD ligand |
| title_full | The targeted delivery of anti-metastasis oligonucleotide in breast cancer cells by the nanoliposomes conjugated with RGD ligand |
| title_fullStr | The targeted delivery of anti-metastasis oligonucleotide in breast cancer cells by the nanoliposomes conjugated with RGD ligand |
| title_full_unstemmed | The targeted delivery of anti-metastasis oligonucleotide in breast cancer cells by the nanoliposomes conjugated with RGD ligand |
| title_short | The targeted delivery of anti-metastasis oligonucleotide in breast cancer cells by the nanoliposomes conjugated with RGD ligand |
| title_sort | targeted delivery of anti metastasis oligonucleotide in breast cancer cells by the nanoliposomes conjugated with rgd ligand |
| topic | Breast cancer ETV4 gene Nanoliposome Antisense oligonucleotide RGD ligand |
| url | http://www.sciencedirect.com/science/article/pii/S2667394025000097 |
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