The Effect of Mechanical Ventilation on TASK-1 Expression in the Brain in a Rat Model
Background and Objective. TWIK-related acid-sensitive potassium channel 1 (TASK-1) is closely related to respiratory central control and neuronal injury. We investigated the effect of MV on TASK-1’s functions and explored the mechanism using a rat model. Methods. Male Sprague-Dawley rats were random...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Canadian Respiratory Journal |
| Online Access: | http://dx.doi.org/10.1155/2017/8530352 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832555132926033920 |
|---|---|
| author | Buqi Na Hong Zhang Guangfa Wang Li Dai Guoguang Xia |
| author_facet | Buqi Na Hong Zhang Guangfa Wang Li Dai Guoguang Xia |
| author_sort | Buqi Na |
| collection | DOAJ |
| description | Background and Objective. TWIK-related acid-sensitive potassium channel 1 (TASK-1) is closely related to respiratory central control and neuronal injury. We investigated the effect of MV on TASK-1’s functions and explored the mechanism using a rat model. Methods. Male Sprague-Dawley rats were randomized to three groups: (1) high tidal volume (HVt): MV for four hours with Vt at 10 mL/kg; (2) low Vt (LVt): MV for four hours with Vt at 5 mL/kg; (3) basal (BAS): anesthetized and unventilated animals. We measured lung histology and plasma and brain levels of proteins (IL-6, TNF-α, and S-100B) and determined TASK-1 levels in rat brainstems as a marker of respiratory centre activity. Results. The LISs (lung injury scores) were significantly higher in the HVt group. Brain inflammatory cytokines levels were different to those in serum. TASK-1 levels were significantly lower in the MV groups (P=0.002) and the HVt group tended to have a lower level of TASK-1 than the LVt group. Conclusion. MV causes not only lung injury, but also brain injury. MV affects the regulation of the respiratory centre, perhaps causing damage to it. Inflammation is probably not the main mechanism of ventilator-related brain injury. |
| format | Article |
| id | doaj-art-c908df92f765475cb56aae8eedfc425a |
| institution | Kabale University |
| issn | 1198-2241 1916-7245 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Respiratory Journal |
| spelling | doaj-art-c908df92f765475cb56aae8eedfc425a2025-02-03T05:49:32ZengWileyCanadian Respiratory Journal1198-22411916-72452017-01-01201710.1155/2017/85303528530352The Effect of Mechanical Ventilation on TASK-1 Expression in the Brain in a Rat ModelBuqi Na0Hong Zhang1Guangfa Wang2Li Dai3Guoguang Xia4Department of Respiratory and Critical Care Medicine, Beijing Jishuitan Hospital, Beijing 100035, ChinaDepartment of Respiratory and Critical Care Medicine, Peking University First Hospital, Beijing 100034, ChinaDepartment of Respiratory and Critical Care Medicine, Peking University First Hospital, Beijing 100034, ChinaDepartment of Respiratory and Critical Care Medicine, Beijing Jishuitan Hospital, Beijing 100035, ChinaDepartment of Respiratory and Critical Care Medicine, Beijing Jishuitan Hospital, Beijing 100035, ChinaBackground and Objective. TWIK-related acid-sensitive potassium channel 1 (TASK-1) is closely related to respiratory central control and neuronal injury. We investigated the effect of MV on TASK-1’s functions and explored the mechanism using a rat model. Methods. Male Sprague-Dawley rats were randomized to three groups: (1) high tidal volume (HVt): MV for four hours with Vt at 10 mL/kg; (2) low Vt (LVt): MV for four hours with Vt at 5 mL/kg; (3) basal (BAS): anesthetized and unventilated animals. We measured lung histology and plasma and brain levels of proteins (IL-6, TNF-α, and S-100B) and determined TASK-1 levels in rat brainstems as a marker of respiratory centre activity. Results. The LISs (lung injury scores) were significantly higher in the HVt group. Brain inflammatory cytokines levels were different to those in serum. TASK-1 levels were significantly lower in the MV groups (P=0.002) and the HVt group tended to have a lower level of TASK-1 than the LVt group. Conclusion. MV causes not only lung injury, but also brain injury. MV affects the regulation of the respiratory centre, perhaps causing damage to it. Inflammation is probably not the main mechanism of ventilator-related brain injury.http://dx.doi.org/10.1155/2017/8530352 |
| spellingShingle | Buqi Na Hong Zhang Guangfa Wang Li Dai Guoguang Xia The Effect of Mechanical Ventilation on TASK-1 Expression in the Brain in a Rat Model Canadian Respiratory Journal |
| title | The Effect of Mechanical Ventilation on TASK-1 Expression in the Brain in a Rat Model |
| title_full | The Effect of Mechanical Ventilation on TASK-1 Expression in the Brain in a Rat Model |
| title_fullStr | The Effect of Mechanical Ventilation on TASK-1 Expression in the Brain in a Rat Model |
| title_full_unstemmed | The Effect of Mechanical Ventilation on TASK-1 Expression in the Brain in a Rat Model |
| title_short | The Effect of Mechanical Ventilation on TASK-1 Expression in the Brain in a Rat Model |
| title_sort | effect of mechanical ventilation on task 1 expression in the brain in a rat model |
| url | http://dx.doi.org/10.1155/2017/8530352 |
| work_keys_str_mv | AT buqina theeffectofmechanicalventilationontask1expressioninthebraininaratmodel AT hongzhang theeffectofmechanicalventilationontask1expressioninthebraininaratmodel AT guangfawang theeffectofmechanicalventilationontask1expressioninthebraininaratmodel AT lidai theeffectofmechanicalventilationontask1expressioninthebraininaratmodel AT guoguangxia theeffectofmechanicalventilationontask1expressioninthebraininaratmodel AT buqina effectofmechanicalventilationontask1expressioninthebraininaratmodel AT hongzhang effectofmechanicalventilationontask1expressioninthebraininaratmodel AT guangfawang effectofmechanicalventilationontask1expressioninthebraininaratmodel AT lidai effectofmechanicalventilationontask1expressioninthebraininaratmodel AT guoguangxia effectofmechanicalventilationontask1expressioninthebraininaratmodel |