In vitro neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves
This study was designed to determine the neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves in vitro. The total phenol and flavonoid contents of the extracts were determined using colorimetric method while phenolic characterization of the leaf was analyzed via hi...
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Tsinghua University Press
2016-06-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453016300350 |
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| author | Ganiyu Oboh Esther E. Nwanna Sunday I. Oyeleye Tosin A. Olasehinde Opeyemi B. Ogunsuyi Aline A. Boligon |
| author_facet | Ganiyu Oboh Esther E. Nwanna Sunday I. Oyeleye Tosin A. Olasehinde Opeyemi B. Ogunsuyi Aline A. Boligon |
| author_sort | Ganiyu Oboh |
| collection | DOAJ |
| description | This study was designed to determine the neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves in vitro. The total phenol and flavonoid contents of the extracts were determined using colorimetric method while phenolic characterization of the leaf was analyzed via high performance liquid chromatography-diode array detector (HPLC-DAD). The effects of the extracts on Fe2+-induced lipid peroxidation in rats’ brain homogenate, monoamine oxidase (MAO), Na+/K+-ATPase, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities were also assessed. The aqueous extract had higher total phenol and flavonoid contents than the methanol extract. HPLC-DAD revealed that quercetin ellagic, chlorogenic and caffeic acids were the most abundant phenolic compounds in the leaves. The aqueous extract had higher inhibitory effects on MAO, AChE and BChE activities while there was no significant difference between their Fe2+-induced lipid peroxidation inhibitory effects. Furthermore, both extracts stimulated Na+/K+-ATPase activity; however, methanol extract had higher stimulatory effect. The neuroprotective properties of H. crinita leaves could be associated with its inhibitory effects on Fe2+-induced lipid peroxidation and modulation of MAO, Na+/K+-ATPase, AChE, and BChE activities. Therefore, H. crinita leaves could be used as a functional food and dietary intervention for the management of some neurodegenerative diseases. Nevertheless, the aqueous extracts exhibited better neuroprotective properties. |
| format | Article |
| id | doaj-art-c8fe172b5ffd479a86cd831e5edf7e49 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2016-06-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-c8fe172b5ffd479a86cd831e5edf7e492025-02-03T06:35:00ZengTsinghua University PressFood Science and Human Wellness2213-45302016-06-01529510210.1016/j.fshw.2016.03.001In vitro neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leavesGaniyu Oboh0Esther E. Nwanna1Sunday I. Oyeleye2Tosin A. Olasehinde3Opeyemi B. Ogunsuyi4Aline A. Boligon5Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, Akure, NigeriaFunctional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, Akure, NigeriaFunctional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, Akure, NigeriaNutrition and Toxicology Division, Food Technology Department, Federal Institute of Industrial Research Oshodi, P.M.B. 21023 Lagos, NigeriaFunctional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, Akure, NigeriaPhytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Build 26, Room 1115, Santa Maria CEP 97105-900, BrazilThis study was designed to determine the neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves in vitro. The total phenol and flavonoid contents of the extracts were determined using colorimetric method while phenolic characterization of the leaf was analyzed via high performance liquid chromatography-diode array detector (HPLC-DAD). The effects of the extracts on Fe2+-induced lipid peroxidation in rats’ brain homogenate, monoamine oxidase (MAO), Na+/K+-ATPase, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities were also assessed. The aqueous extract had higher total phenol and flavonoid contents than the methanol extract. HPLC-DAD revealed that quercetin ellagic, chlorogenic and caffeic acids were the most abundant phenolic compounds in the leaves. The aqueous extract had higher inhibitory effects on MAO, AChE and BChE activities while there was no significant difference between their Fe2+-induced lipid peroxidation inhibitory effects. Furthermore, both extracts stimulated Na+/K+-ATPase activity; however, methanol extract had higher stimulatory effect. The neuroprotective properties of H. crinita leaves could be associated with its inhibitory effects on Fe2+-induced lipid peroxidation and modulation of MAO, Na+/K+-ATPase, AChE, and BChE activities. Therefore, H. crinita leaves could be used as a functional food and dietary intervention for the management of some neurodegenerative diseases. Nevertheless, the aqueous extracts exhibited better neuroprotective properties.http://www.sciencedirect.com/science/article/pii/S2213453016300350Heinsia crinitaNeurodegenerationOxidative stressMalondialdehydePolyphenols |
| spellingShingle | Ganiyu Oboh Esther E. Nwanna Sunday I. Oyeleye Tosin A. Olasehinde Opeyemi B. Ogunsuyi Aline A. Boligon In vitro neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves Food Science and Human Wellness Heinsia crinita Neurodegeneration Oxidative stress Malondialdehyde Polyphenols |
| title | In vitro neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves |
| title_full | In vitro neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves |
| title_fullStr | In vitro neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves |
| title_full_unstemmed | In vitro neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves |
| title_short | In vitro neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves |
| title_sort | in vitro neuroprotective potentials of aqueous and methanol extracts from heinsia crinita leaves |
| topic | Heinsia crinita Neurodegeneration Oxidative stress Malondialdehyde Polyphenols |
| url | http://www.sciencedirect.com/science/article/pii/S2213453016300350 |
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