Correlation between Olink and SomaScan proteomics platforms in adults with a Fontan circulation
Background: High-throughput proteomics platforms using aptamers (SomaScan) or proximity extension assay (Olink) provide novel opportunities for improving diagnostic and risk stratification tools in cardiovascular diseases, including understudied congenital heart diseases. The correlation between the...
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Elsevier
2025-06-01
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| Series: | International Journal of Cardiology Congenital Heart Disease |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666668525000205 |
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| author | Ismael Z. Assi Michael J. Landzberg Kristian C. Becker David Renaud Fernando Baraona Reyes David M. Leone Mark Benson Miriam Michel Robert E. Gerszten Alexander R. Opotowsky |
| author_facet | Ismael Z. Assi Michael J. Landzberg Kristian C. Becker David Renaud Fernando Baraona Reyes David M. Leone Mark Benson Miriam Michel Robert E. Gerszten Alexander R. Opotowsky |
| author_sort | Ismael Z. Assi |
| collection | DOAJ |
| description | Background: High-throughput proteomics platforms using aptamers (SomaScan) or proximity extension assay (Olink) provide novel opportunities for improving diagnostic and risk stratification tools in cardiovascular diseases, including understudied congenital heart diseases. The correlation between these proteomics approaches has not yet been studied among individuals with a Fontan circulation. Objective: The correlation of plasma protein measurements between SomaScan and Olink platforms was evaluated in adults with a Fontan circulation. Methods: We measured 491 proteins in plasma of 71 adults with a Fontan circulation using Olink and SomaScan. Missing Olink measurements (0.13%, 47/34,861) were imputed using non-parametric imputation. Spearman's rank correlation coefficient for absolute values of protein expression between platforms was calculated. Protein correlation frequencies were compared to 3 cohorts reported in the literature using Pearson's Chi-squared test of independence. Results: Overall, protein correlations between Olink and SomaScan measurements were moderately strong for most proteins, (rho > 0.4 for 57.2%), but with substantial variability (median correlation = 0.457, IQR = 0.538). The distribution of protein correlations was qualitatively similar to published literature in non-Fontan cohorts. Both Olink and SomaScan identified proteins with sex-based differences; both identified differences in myostatin and leptin, but each identified additional nonoverlapping sexually dimorphic proteins (n = 14 Olink, n = 5 SomaScan). Conclusions: In adults with a Fontan circulation, correlations between plasma proteins measured by Olink and SomaScan varied widely, approximately in line with prior reports in other populations. While these tools may be uniquely useful to generate hypotheses, specifically regarding potential molecular mechanisms, more definitive inference requires independent validation. |
| format | Article |
| id | doaj-art-c8f794cd2bbe44a1ae7c206fb421c1e9 |
| institution | DOAJ |
| issn | 2666-6685 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Cardiology Congenital Heart Disease |
| spelling | doaj-art-c8f794cd2bbe44a1ae7c206fb421c1e92025-08-20T03:14:24ZengElsevierInternational Journal of Cardiology Congenital Heart Disease2666-66852025-06-012010058410.1016/j.ijcchd.2025.100584Correlation between Olink and SomaScan proteomics platforms in adults with a Fontan circulationIsmael Z. Assi0Michael J. Landzberg1Kristian C. Becker2David Renaud3Fernando Baraona Reyes4David M. Leone5Mark Benson6Miriam Michel7Robert E. Gerszten8Alexander R. Opotowsky9Heart Institute, Department of Pediatrics, Cincinnati Children's Hospital, University of Cincinnati College of Medicine, Cincinnati, OH, USADepartment of Cardiology, Boston Children's Hospital, Boston, MA, USA; Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USAHeart Institute, Department of Pediatrics, Cincinnati Children's Hospital, University of Cincinnati College of Medicine, Cincinnati, OH, USAFundamental and Biomedical Sciences, Paris-Cité University, Paris, France; Health Sciences Faculty, Universidad Europea Miguel de Cervantes, Valladolid, SpainDepartment of Cardiology, Boston Children's Hospital, Boston, MA, USA; Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA; Harvard Medical School, Boston, MA, USAHeart Institute, Department of Pediatrics, Cincinnati Children's Hospital, University of Cincinnati College of Medicine, Cincinnati, OH, USAHarvard Medical School, Boston, MA, USA; CardioVascular Institute, Beth Israel Deaconess Medical Center, Boston, MA, USA; Division of Cardiovascular Medicine, Beth Israel Deaconess Medical Center, Boston, MA, USADepartment of Child and Adolescent Health, Division of Pediatrics III — Cardiology, Pulmonology, Allergology and Cystic Fibrosis, Medical University of Innsbruck, Innsbruck, AustriaHarvard Medical School, Boston, MA, USA; CardioVascular Institute, Beth Israel Deaconess Medical Center, Boston, MA, USA; Division of Cardiovascular Medicine, Beth Israel Deaconess Medical Center, Boston, MA, USAHeart Institute, Department of Pediatrics, Cincinnati Children's Hospital, University of Cincinnati College of Medicine, Cincinnati, OH, USA; Department of Cardiology, Boston Children's Hospital, Boston, MA, USA; Corresponding author. Cincinnati Adult Congenital Heart Disease Program Heart Institute, Cincinnati Children's Hospital 3333 Burnet Avenue, MLC 2003, Cincinnati, OH, 45229, USA.Background: High-throughput proteomics platforms using aptamers (SomaScan) or proximity extension assay (Olink) provide novel opportunities for improving diagnostic and risk stratification tools in cardiovascular diseases, including understudied congenital heart diseases. The correlation between these proteomics approaches has not yet been studied among individuals with a Fontan circulation. Objective: The correlation of plasma protein measurements between SomaScan and Olink platforms was evaluated in adults with a Fontan circulation. Methods: We measured 491 proteins in plasma of 71 adults with a Fontan circulation using Olink and SomaScan. Missing Olink measurements (0.13%, 47/34,861) were imputed using non-parametric imputation. Spearman's rank correlation coefficient for absolute values of protein expression between platforms was calculated. Protein correlation frequencies were compared to 3 cohorts reported in the literature using Pearson's Chi-squared test of independence. Results: Overall, protein correlations between Olink and SomaScan measurements were moderately strong for most proteins, (rho > 0.4 for 57.2%), but with substantial variability (median correlation = 0.457, IQR = 0.538). The distribution of protein correlations was qualitatively similar to published literature in non-Fontan cohorts. Both Olink and SomaScan identified proteins with sex-based differences; both identified differences in myostatin and leptin, but each identified additional nonoverlapping sexually dimorphic proteins (n = 14 Olink, n = 5 SomaScan). Conclusions: In adults with a Fontan circulation, correlations between plasma proteins measured by Olink and SomaScan varied widely, approximately in line with prior reports in other populations. While these tools may be uniquely useful to generate hypotheses, specifically regarding potential molecular mechanisms, more definitive inference requires independent validation.http://www.sciencedirect.com/science/article/pii/S2666668525000205Precision medicinePlasma proteomicsOlinkSomaScanFontan circulationAdult congenital heart disease |
| spellingShingle | Ismael Z. Assi Michael J. Landzberg Kristian C. Becker David Renaud Fernando Baraona Reyes David M. Leone Mark Benson Miriam Michel Robert E. Gerszten Alexander R. Opotowsky Correlation between Olink and SomaScan proteomics platforms in adults with a Fontan circulation International Journal of Cardiology Congenital Heart Disease Precision medicine Plasma proteomics Olink SomaScan Fontan circulation Adult congenital heart disease |
| title | Correlation between Olink and SomaScan proteomics platforms in adults with a Fontan circulation |
| title_full | Correlation between Olink and SomaScan proteomics platforms in adults with a Fontan circulation |
| title_fullStr | Correlation between Olink and SomaScan proteomics platforms in adults with a Fontan circulation |
| title_full_unstemmed | Correlation between Olink and SomaScan proteomics platforms in adults with a Fontan circulation |
| title_short | Correlation between Olink and SomaScan proteomics platforms in adults with a Fontan circulation |
| title_sort | correlation between olink and somascan proteomics platforms in adults with a fontan circulation |
| topic | Precision medicine Plasma proteomics Olink SomaScan Fontan circulation Adult congenital heart disease |
| url | http://www.sciencedirect.com/science/article/pii/S2666668525000205 |
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