Transcriptomic analysis of ileal adaptations and growth responses in growing hens supplemented with alanyl-glutamine dipeptide
The growing phase of laying hens is crucial for growth and development due to its direct impact on their productivity during laying phase. During initial growth phase, intestinal tract undergoes rapid development which requires plenty of nutrients to help laying hens grow and mature. This study inve...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124010575 |
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| author | Usman Nazir Zhenming Fu Xucheng Zheng Muhamamd Hammad Zafar Zhi Yang Zhiyue Wang Haiming Yang |
| author_facet | Usman Nazir Zhenming Fu Xucheng Zheng Muhamamd Hammad Zafar Zhi Yang Zhiyue Wang Haiming Yang |
| author_sort | Usman Nazir |
| collection | DOAJ |
| description | The growing phase of laying hens is crucial for growth and development due to its direct impact on their productivity during laying phase. During initial growth phase, intestinal tract undergoes rapid development which requires plenty of nutrients to help laying hens grow and mature. This study investigated the effect of Alanyl-Glutamine (Aln-Gln) levels on growth performance, ileal morphology and transcriptomic analysis of growing Hy-line brown hens. A total of 480 day old Hy-line brown chicks having similar body weight (BW) were randomly divided to be fed diets having 0%, 0.1%, 0.2% and 0.3% Aln-Gln for 6-wks (8 replicates/group, 15 birds/replicate). One bird from every pen was slaughtered and morphological parameters of ileum were evaluated. Results taken on day 42 revealed an improved average daily gain (ADG), final body weight (FBW) and feed-to-gain ratio (F/G) in the birds that consumed 0.2% and 0.3% Aln-Gln supplemented diet (P < 0.05). Ileal morphological assays showed that villus height, villus width and villus to crypts ratio (V/C) were significantly increased at 42 days of age in birds fed diets with 0.2% Aln-Gln (P<0.05). The RNA sequencing (RNA-Seq) was executed to identify differentially expressed genes (DEGs) among groups that found 2265 DEGs (1256 up-regulated; 1009 down-regulated) in ileum tissue. According to the Kyoto Encyclopedia of Genes (KEGG) and Genomic Pathway Enrichment Analysis, majority of DEGs indicated change in metabolic pathways. Genes related to growth factors, intestinal morphology and protein metabolism were up-regulated in test groups as compared to control group. In conclusion, addition of Aln-Gln to the diet improved growth performance and ileum development in growing hens; transcriptomic analysis revealed up-regulation of genes related to growth and intestinal morphology. |
| format | Article |
| id | doaj-art-c8eadaa8c23943f9a080344ae476b4f9 |
| institution | OA Journals |
| issn | 0032-5791 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-c8eadaa8c23943f9a080344ae476b4f92025-08-20T01:55:37ZengElsevierPoultry Science0032-57912024-12-011031210447910.1016/j.psj.2024.104479Transcriptomic analysis of ileal adaptations and growth responses in growing hens supplemented with alanyl-glutamine dipeptideUsman Nazir0Zhenming Fu1Xucheng Zheng2Muhamamd Hammad Zafar3Zhi Yang4Zhiyue Wang5Haiming Yang6Yangzhou University, College of Animal Science and Technology, 225009 Yangzhou, ChinaYangzhou University, College of Animal Science and Technology, 225009 Yangzhou, ChinaYangzhou University, College of Animal Science and Technology, 225009 Yangzhou, ChinaYangzhou University, College of Animal Science and Technology, 225009 Yangzhou, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaYangzhou University, College of Animal Science and Technology, 225009 Yangzhou, ChinaYangzhou University, College of Animal Science and Technology, 225009 Yangzhou, China; Corresponding author at: 48 Wenhui East Road, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, China.The growing phase of laying hens is crucial for growth and development due to its direct impact on their productivity during laying phase. During initial growth phase, intestinal tract undergoes rapid development which requires plenty of nutrients to help laying hens grow and mature. This study investigated the effect of Alanyl-Glutamine (Aln-Gln) levels on growth performance, ileal morphology and transcriptomic analysis of growing Hy-line brown hens. A total of 480 day old Hy-line brown chicks having similar body weight (BW) were randomly divided to be fed diets having 0%, 0.1%, 0.2% and 0.3% Aln-Gln for 6-wks (8 replicates/group, 15 birds/replicate). One bird from every pen was slaughtered and morphological parameters of ileum were evaluated. Results taken on day 42 revealed an improved average daily gain (ADG), final body weight (FBW) and feed-to-gain ratio (F/G) in the birds that consumed 0.2% and 0.3% Aln-Gln supplemented diet (P < 0.05). Ileal morphological assays showed that villus height, villus width and villus to crypts ratio (V/C) were significantly increased at 42 days of age in birds fed diets with 0.2% Aln-Gln (P<0.05). The RNA sequencing (RNA-Seq) was executed to identify differentially expressed genes (DEGs) among groups that found 2265 DEGs (1256 up-regulated; 1009 down-regulated) in ileum tissue. According to the Kyoto Encyclopedia of Genes (KEGG) and Genomic Pathway Enrichment Analysis, majority of DEGs indicated change in metabolic pathways. Genes related to growth factors, intestinal morphology and protein metabolism were up-regulated in test groups as compared to control group. In conclusion, addition of Aln-Gln to the diet improved growth performance and ileum development in growing hens; transcriptomic analysis revealed up-regulation of genes related to growth and intestinal morphology.http://www.sciencedirect.com/science/article/pii/S0032579124010575GlutamineIleumGrowthTranscriptomic analysisGene expression |
| spellingShingle | Usman Nazir Zhenming Fu Xucheng Zheng Muhamamd Hammad Zafar Zhi Yang Zhiyue Wang Haiming Yang Transcriptomic analysis of ileal adaptations and growth responses in growing hens supplemented with alanyl-glutamine dipeptide Poultry Science Glutamine Ileum Growth Transcriptomic analysis Gene expression |
| title | Transcriptomic analysis of ileal adaptations and growth responses in growing hens supplemented with alanyl-glutamine dipeptide |
| title_full | Transcriptomic analysis of ileal adaptations and growth responses in growing hens supplemented with alanyl-glutamine dipeptide |
| title_fullStr | Transcriptomic analysis of ileal adaptations and growth responses in growing hens supplemented with alanyl-glutamine dipeptide |
| title_full_unstemmed | Transcriptomic analysis of ileal adaptations and growth responses in growing hens supplemented with alanyl-glutamine dipeptide |
| title_short | Transcriptomic analysis of ileal adaptations and growth responses in growing hens supplemented with alanyl-glutamine dipeptide |
| title_sort | transcriptomic analysis of ileal adaptations and growth responses in growing hens supplemented with alanyl glutamine dipeptide |
| topic | Glutamine Ileum Growth Transcriptomic analysis Gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124010575 |
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