Systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespan
Abstract Aging is a multifaceted biological process marked by the decline in both mitotic and postmitotic cellular function, often central to the development of age-related diseases. In the pursuit of slowing or even reversing the aging process, a prominent strategy of significant interest is calori...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-05-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-08178-6 |
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| author | Yizhong Zhang Arshia Naaz Trishia Yi Ning Cheng Jovian Jing Lin Mingtong Gao Rajkumar Dorajoo Mohammad Alfatah |
| author_facet | Yizhong Zhang Arshia Naaz Trishia Yi Ning Cheng Jovian Jing Lin Mingtong Gao Rajkumar Dorajoo Mohammad Alfatah |
| author_sort | Yizhong Zhang |
| collection | DOAJ |
| description | Abstract Aging is a multifaceted biological process marked by the decline in both mitotic and postmitotic cellular function, often central to the development of age-related diseases. In the pursuit of slowing or even reversing the aging process, a prominent strategy of significant interest is calorie restriction (CR), also known as dietary restriction, and the potential influence of a drug called rapamycin (RM). Both CR and RM have demonstrated the capacity to extend healthspan and lifespan across a diverse array of species, including yeast, worms, flies, and mice. Nevertheless, their individual and combined effects on mitotic and postmitotic cells, as well as their comparative analysis, remain areas that demand a thorough investigation. In this study, we employ RNA-sequencing methodologies to comprehensively analyze the impact of CR, RM, and their combination (CR + RM) on gene expression in yeast cells. Our analysis uncovers distinctive, overlapping, and even contrasting patterns of gene regulation, illuminating the unique and shared effects of CR and RM. Furthermore, the transcriptional synergistic interaction of CR + RM is validated in extending the lifespan of both yeast and human cells. |
| format | Article |
| id | doaj-art-c8e49002eef64e73871785ac5c6fe8ff |
| institution | OA Journals |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-c8e49002eef64e73871785ac5c6fe8ff2025-08-20T02:25:12ZengNature PortfolioCommunications Biology2399-36422025-05-018111610.1038/s42003-025-08178-6Systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespanYizhong Zhang0Arshia Naaz1Trishia Yi Ning Cheng2Jovian Jing Lin3Mingtong Gao4Rajkumar Dorajoo5Mohammad Alfatah6Bioinformatics Institute (BII), Agency for Science, Technology and Research (A*STAR)Genome Institute of Singapore (GIS), Agency for Science, Technology and Research (A*STAR)Bioinformatics Institute (BII), Agency for Science, Technology and Research (A*STAR)Bioinformatics Institute (BII), Agency for Science, Technology and Research (A*STAR)Healthy Longevity Translational Research Program, Yong Loo Lin School of Medicine, National University of SingaporeGenome Institute of Singapore (GIS), Agency for Science, Technology and Research (A*STAR)Bioinformatics Institute (BII), Agency for Science, Technology and Research (A*STAR)Abstract Aging is a multifaceted biological process marked by the decline in both mitotic and postmitotic cellular function, often central to the development of age-related diseases. In the pursuit of slowing or even reversing the aging process, a prominent strategy of significant interest is calorie restriction (CR), also known as dietary restriction, and the potential influence of a drug called rapamycin (RM). Both CR and RM have demonstrated the capacity to extend healthspan and lifespan across a diverse array of species, including yeast, worms, flies, and mice. Nevertheless, their individual and combined effects on mitotic and postmitotic cells, as well as their comparative analysis, remain areas that demand a thorough investigation. In this study, we employ RNA-sequencing methodologies to comprehensively analyze the impact of CR, RM, and their combination (CR + RM) on gene expression in yeast cells. Our analysis uncovers distinctive, overlapping, and even contrasting patterns of gene regulation, illuminating the unique and shared effects of CR and RM. Furthermore, the transcriptional synergistic interaction of CR + RM is validated in extending the lifespan of both yeast and human cells.https://doi.org/10.1038/s42003-025-08178-6 |
| spellingShingle | Yizhong Zhang Arshia Naaz Trishia Yi Ning Cheng Jovian Jing Lin Mingtong Gao Rajkumar Dorajoo Mohammad Alfatah Systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespan Communications Biology |
| title | Systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespan |
| title_full | Systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespan |
| title_fullStr | Systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespan |
| title_full_unstemmed | Systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespan |
| title_short | Systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespan |
| title_sort | systematic transcriptomics analysis of calorie restriction and rapamycin unveils their synergistic interaction in prolonging cellular lifespan |
| url | https://doi.org/10.1038/s42003-025-08178-6 |
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