The Peroxisome Proliferator-Activated Receptor Gamma System Regulates Ultraviolet B-Induced Prostaglandin E2 Production in Human Epidermal Keratinocytes
Studies using PPARγ agonists in mouse skin have suggested that peroxisome proliferator-activated receptor gamma (PPARγ) is irrelevant to cutaneous photobiology. However, in several epithelial cell lines, ultraviolet B (UVB) has been shown to induce the nonenzymatic production of oxidized phospholip...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2010/467053 |
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| author | Raymond L. Konger Kellie Clay Martel Danielle Jernigan Qiwei Zhang Jeffrey B. Travers |
| author_facet | Raymond L. Konger Kellie Clay Martel Danielle Jernigan Qiwei Zhang Jeffrey B. Travers |
| author_sort | Raymond L. Konger |
| collection | DOAJ |
| description | Studies using PPARγ agonists in mouse skin have suggested that peroxisome proliferator-activated receptor gamma (PPARγ) is irrelevant to cutaneous photobiology. However, in several epithelial cell lines, ultraviolet B (UVB) has been shown to induce the nonenzymatic production of oxidized phospholipids that act as PPARγ agonists. UVB is also a potent inducer of prostaglandin E2 (PGE2) production and COX-2 expression in keratinocytes and PPARγ is coupled to increased PGE2 production in other cell lines. In this current study, we demonstrate that PPARγ agonists, but not PPARα or PPARβ/δ agonists, induce PGE2 production and COX-2 expression in primary human keratinocytes (PHKs). Importantly, PPARγ agonist-induced COX-2 expression and PGE2 production were partially inhibited by the PPARγ antagonist, GW9662, indicating that both PPARγ-dependent and -independent pathways are likely involved. GW9662 also suppressed UVB and tert-butylhydroperoxide- (TBH-) induced PGE2 production in PHKs and intact human epidermis and partially inhibited UVB-induced COX-2 expression in PHKs. These findings provide evidence that PPARγ is relevant to cutaneous photobiology in human epidermis. |
| format | Article |
| id | doaj-art-c8e2f8a4ce6142f0a4984a854a78d466 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-c8e2f8a4ce6142f0a4984a854a78d4662025-02-03T06:08:48ZengWileyPPAR Research1687-47571687-47652010-01-01201010.1155/2010/467053467053The Peroxisome Proliferator-Activated Receptor Gamma System Regulates Ultraviolet B-Induced Prostaglandin E2 Production in Human Epidermal KeratinocytesRaymond L. Konger0Kellie Clay Martel1Danielle Jernigan2Qiwei Zhang3Jeffrey B. Travers4Department of Pathology & Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Pathology & Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Pathology & Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Dermatology, Indiana University School of Medicine, Indianapolis, IN 46202, USADepartment of Dermatology, Indiana University School of Medicine, Indianapolis, IN 46202, USAStudies using PPARγ agonists in mouse skin have suggested that peroxisome proliferator-activated receptor gamma (PPARγ) is irrelevant to cutaneous photobiology. However, in several epithelial cell lines, ultraviolet B (UVB) has been shown to induce the nonenzymatic production of oxidized phospholipids that act as PPARγ agonists. UVB is also a potent inducer of prostaglandin E2 (PGE2) production and COX-2 expression in keratinocytes and PPARγ is coupled to increased PGE2 production in other cell lines. In this current study, we demonstrate that PPARγ agonists, but not PPARα or PPARβ/δ agonists, induce PGE2 production and COX-2 expression in primary human keratinocytes (PHKs). Importantly, PPARγ agonist-induced COX-2 expression and PGE2 production were partially inhibited by the PPARγ antagonist, GW9662, indicating that both PPARγ-dependent and -independent pathways are likely involved. GW9662 also suppressed UVB and tert-butylhydroperoxide- (TBH-) induced PGE2 production in PHKs and intact human epidermis and partially inhibited UVB-induced COX-2 expression in PHKs. These findings provide evidence that PPARγ is relevant to cutaneous photobiology in human epidermis.http://dx.doi.org/10.1155/2010/467053 |
| spellingShingle | Raymond L. Konger Kellie Clay Martel Danielle Jernigan Qiwei Zhang Jeffrey B. Travers The Peroxisome Proliferator-Activated Receptor Gamma System Regulates Ultraviolet B-Induced Prostaglandin E2 Production in Human Epidermal Keratinocytes PPAR Research |
| title | The Peroxisome Proliferator-Activated Receptor Gamma System Regulates Ultraviolet B-Induced Prostaglandin E2 Production in Human Epidermal Keratinocytes |
| title_full | The Peroxisome Proliferator-Activated Receptor Gamma System Regulates Ultraviolet B-Induced Prostaglandin E2 Production in Human Epidermal Keratinocytes |
| title_fullStr | The Peroxisome Proliferator-Activated Receptor Gamma System Regulates Ultraviolet B-Induced Prostaglandin E2 Production in Human Epidermal Keratinocytes |
| title_full_unstemmed | The Peroxisome Proliferator-Activated Receptor Gamma System Regulates Ultraviolet B-Induced Prostaglandin E2 Production in Human Epidermal Keratinocytes |
| title_short | The Peroxisome Proliferator-Activated Receptor Gamma System Regulates Ultraviolet B-Induced Prostaglandin E2 Production in Human Epidermal Keratinocytes |
| title_sort | peroxisome proliferator activated receptor gamma system regulates ultraviolet b induced prostaglandin e2 production in human epidermal keratinocytes |
| url | http://dx.doi.org/10.1155/2010/467053 |
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