Genome-wide analysis of the DnaJ gene family and their expression profiles under salt stress in potato
Abstract Background Saline stress is one of the primary environmental factors affecting global food security, significantly jeopardizing crop quality and yield. DnaJ proteins (DnaJs) are a type of protein that plays an important role in dealing with salt stress for plants. Nevertheless, the comprehe...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-08-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-025-11961-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849226603103518720 |
|---|---|
| author | Qi Fu Yanan Pu Jintao Liu Xuemei Wang Dianjue Li Guangtao Zhu |
| author_facet | Qi Fu Yanan Pu Jintao Liu Xuemei Wang Dianjue Li Guangtao Zhu |
| author_sort | Qi Fu |
| collection | DOAJ |
| description | Abstract Background Saline stress is one of the primary environmental factors affecting global food security, significantly jeopardizing crop quality and yield. DnaJ proteins (DnaJs) are a type of protein that plays an important role in dealing with salt stress for plants. Nevertheless, the comprehensive exploration of the DnaJ gene family and its role in salt stress in potato (Solanum tuberosum L.) is rare. Results A total of 113 StDnaJs were identified from the potato genome, and they were categorized into three distinct types, with the same type members possessing similar structures. Gene duplication analysis identified twenty-five pairs of duplicated genes, potentially contributing to the expansion of the StDnaJ family in potato. The promoters of StDnaJ genes contained numerous cis-acting regulatory elements linked to light response, plant growth, hormone response, and stress response. A heat map was generated using RNA-seq data, suggesting that they were expressed in roots or leaves under salt stress. We also selected 22 highly responsive members with continuous induction to validate their expression patterns using RT-qPCR, finding that they were significantly induced by salt stress in leaves or roots, with StDnaJ10, StDnaJ21, StDnaJ23, StDnaJ64, StDnaJ94, and StDnaJ107 being induced in both roots and leaves, indicating that these genes may play important roles in salt stress. Conclusions This study presents an extensive analysis of StDnaJs and provides targeted members for further salt-resistant research in potato. |
| format | Article |
| id | doaj-art-c8e16f64de07440f810ada899eaa9a57 |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-c8e16f64de07440f810ada899eaa9a572025-08-24T11:09:36ZengBMCBMC Genomics1471-21642025-08-0126111310.1186/s12864-025-11961-8Genome-wide analysis of the DnaJ gene family and their expression profiles under salt stress in potatoQi Fu0Yanan Pu1Jintao Liu2Xuemei Wang3Dianjue Li4Guangtao Zhu5Yunnan Key Laboratory of Potato Biology, The AGISCAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal UniversityYunnan Key Laboratory of Potato Biology, The AGISCAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal UniversityYunnan Key Laboratory of Potato Biology, The AGISCAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal UniversityYunnan Key Laboratory of Potato Biology, The AGISCAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal UniversityYunnan Key Laboratory of Potato Biology, The AGISCAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal UniversityYunnan Key Laboratory of Potato Biology, The AGISCAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal UniversityAbstract Background Saline stress is one of the primary environmental factors affecting global food security, significantly jeopardizing crop quality and yield. DnaJ proteins (DnaJs) are a type of protein that plays an important role in dealing with salt stress for plants. Nevertheless, the comprehensive exploration of the DnaJ gene family and its role in salt stress in potato (Solanum tuberosum L.) is rare. Results A total of 113 StDnaJs were identified from the potato genome, and they were categorized into three distinct types, with the same type members possessing similar structures. Gene duplication analysis identified twenty-five pairs of duplicated genes, potentially contributing to the expansion of the StDnaJ family in potato. The promoters of StDnaJ genes contained numerous cis-acting regulatory elements linked to light response, plant growth, hormone response, and stress response. A heat map was generated using RNA-seq data, suggesting that they were expressed in roots or leaves under salt stress. We also selected 22 highly responsive members with continuous induction to validate their expression patterns using RT-qPCR, finding that they were significantly induced by salt stress in leaves or roots, with StDnaJ10, StDnaJ21, StDnaJ23, StDnaJ64, StDnaJ94, and StDnaJ107 being induced in both roots and leaves, indicating that these genes may play important roles in salt stress. Conclusions This study presents an extensive analysis of StDnaJs and provides targeted members for further salt-resistant research in potato.https://doi.org/10.1186/s12864-025-11961-8PotatoDnaJ gene familySalt stressExpression patterns |
| spellingShingle | Qi Fu Yanan Pu Jintao Liu Xuemei Wang Dianjue Li Guangtao Zhu Genome-wide analysis of the DnaJ gene family and their expression profiles under salt stress in potato BMC Genomics Potato DnaJ gene family Salt stress Expression patterns |
| title | Genome-wide analysis of the DnaJ gene family and their expression profiles under salt stress in potato |
| title_full | Genome-wide analysis of the DnaJ gene family and their expression profiles under salt stress in potato |
| title_fullStr | Genome-wide analysis of the DnaJ gene family and their expression profiles under salt stress in potato |
| title_full_unstemmed | Genome-wide analysis of the DnaJ gene family and their expression profiles under salt stress in potato |
| title_short | Genome-wide analysis of the DnaJ gene family and their expression profiles under salt stress in potato |
| title_sort | genome wide analysis of the dnaj gene family and their expression profiles under salt stress in potato |
| topic | Potato DnaJ gene family Salt stress Expression patterns |
| url | https://doi.org/10.1186/s12864-025-11961-8 |
| work_keys_str_mv | AT qifu genomewideanalysisofthednajgenefamilyandtheirexpressionprofilesundersaltstressinpotato AT yananpu genomewideanalysisofthednajgenefamilyandtheirexpressionprofilesundersaltstressinpotato AT jintaoliu genomewideanalysisofthednajgenefamilyandtheirexpressionprofilesundersaltstressinpotato AT xuemeiwang genomewideanalysisofthednajgenefamilyandtheirexpressionprofilesundersaltstressinpotato AT dianjueli genomewideanalysisofthednajgenefamilyandtheirexpressionprofilesundersaltstressinpotato AT guangtaozhu genomewideanalysisofthednajgenefamilyandtheirexpressionprofilesundersaltstressinpotato |