The Combination of Curcumae Radix and Syzygium Aromaticum Extracts Mitigates Benign Prostatic Hyperplasia through Anti-Proliferative and Anti-Inflammatory Effects

Purpose: Benign prostatic hyperplasia (BPH) is a urinary tract disorder that primarily affects geriatric males. Natural materials have been developed to treat and prevent symptoms of BPH. However, a few natural functional foods has been conclusively proven to cure or prevent symptoms of BPH. The s...

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Main Authors: Geum Hwa Lee, Yu Seob Shin, Ji Hyun Kim, Da hee Shim, Hwa Young Lee, Luo Zhao, Mohammad Mamun Ur Rashid, Hwang Eui Cho, Jong Jin Lee, Min Kyoung Kim, Do Sung Kim, Hyun Jun Park, Han Jung Chae
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Language:English
Published: Korean Society for Sexual Medicine and Andrology 2025-07-01
Series:The World Journal of Men's Health
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author Geum Hwa Lee
Yu Seob Shin
Ji Hyun Kim
Da hee Shim
Hwa Young Lee
Luo Zhao
Mohammad Mamun Ur Rashid
Hwang Eui Cho
Jong Jin Lee
Min Kyoung Kim
Do Sung Kim
Hyun Jun Park
Han Jung Chae
author_facet Geum Hwa Lee
Yu Seob Shin
Ji Hyun Kim
Da hee Shim
Hwa Young Lee
Luo Zhao
Mohammad Mamun Ur Rashid
Hwang Eui Cho
Jong Jin Lee
Min Kyoung Kim
Do Sung Kim
Hyun Jun Park
Han Jung Chae
author_sort Geum Hwa Lee
collection DOAJ
description Purpose: Benign prostatic hyperplasia (BPH) is a urinary tract disorder that primarily affects geriatric males. Natural materials have been developed to treat and prevent symptoms of BPH. However, a few natural functional foods has been conclusively proven to cure or prevent symptoms of BPH. The study aimed to investigate the anti-proliferative mechanism of Curcumae Radix (CR) and Syzygium aromaticum (SA) extracts using RWPE-1 cells and testosterone propionate (TP)-induced BPH rats. Materials and Methods: In vitro experiments were performed to assess the synergistic anti-proliferative effects of an equal mixture of CR and SA extracts on TP-treated RWPE-1 cells. In animal experiments, TP-induced BPH rats were administrated with saline, CR and SA extracts at 50, 100, and 200 mg/kg or finasteride at 1 mg/kg daily for 6 weeks. Body weight, prostate weight, dihydrotestosterone (DHT), androgen receptor (AR), and prostate-specific antigen (PSA) levels were measured. Additionally, extracellular signal-regulated kinase and NF-κB levels, oxidative stress, and inflammatory stress responses in the prostate were also analyzed. Results: In this study, the combination of CR and SA extracts synergistically inhibited cell proliferation compared with the effect of each extract in TP-treated RWPE-1 cells. CR and SA extracts inhibited increasing of prostate weight, thickness of prostate epithelium, the level of PSA and DHT in serum. The expression of protein and gene of PSA and AR in prostate of TPinduced BPH rats were also suppressed by the administration of CR and SA extracts. Furthermore, reactive oxygen species and inflammation axis, NOX4-iNOS-COX2 and its associated representative inflammatory cytokine, interleukin-8 were also reduced in the CR and SA extracts-administered BPH rats. Conclusions: The results suggest that the combination of CR and SA extracts improves BPH through its anti-inflammatory and anti-oxidative effects, demonstrating great potential as an anti-BPH medicine.
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spelling doaj-art-c8d3e5fd0e6849e587eea04e38b653a32025-08-20T03:24:22ZengKorean Society for Sexual Medicine and AndrologyThe World Journal of Men's Health2287-42082287-46902025-07-0143360361510.5534/wjmh.240071The Combination of Curcumae Radix and Syzygium Aromaticum Extracts Mitigates Benign Prostatic Hyperplasia through Anti-Proliferative and Anti-Inflammatory EffectsGeum Hwa Lee0https://orcid.org/0000-0002-9056-0073Yu Seob Shin1https://orcid.org/0000-0002-1126-3821Ji Hyun Kim2https://orcid.org/0009-0000-8901-1360Da hee Shim3https://orcid.org/0000-0002-7481-7131Hwa Young Lee4https://orcid.org/0000-0001-9365-5863Luo Zhao5https://orcid.org/0009-0005-7807-6296Mohammad Mamun Ur Rashid6https://orcid.org/0000-0002-7025-1716Hwang Eui Cho7https://orcid.org/0000-0001-9220-7225Jong Jin Lee8https://orcid.org/0009-0004-8043-9810Min Kyoung Kim9https://orcid.org/0009-0009-0328-5025Do Sung Kim10https://orcid.org/0000-0002-7739-6256Hyun Jun Park11https://orcid.org/0000-0003-0566-9574Han Jung Chae12https://orcid.org/0000-0003-4190-9889Research Institute of Clinical Medicine of Jeonbuk National University-Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, KoreaResearch Institute of Clinical Medicine of Jeonbuk National University-Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, KoreaNon-Clinical Evaluation Center, Biomedical Research Institute, Jeonbuk National University Hospital, Jeonju, KoreaIndustry-Academic Cooperation Foundation, Hallym University, Chuncheon, KoreaResearch Institute of Clinical Medicine of Jeonbuk National University-Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, KoreaDepartment of Urology, Jeonbuk National University Medical School, Jeonju, KoreaDepartment of Pharmacology and Institute of New Drug Development, Jeonbuk National University Medical School, Jeonju, KoreaSchool of Pharmacy and Institute of New Drug Development, Jeonbuk National University, Jeonju, KoreaDepartment of Strategic Planning, Whan In Pharm Co., Ltd., Seoul, KoreaDepartment of Strategic Planning, Whan In Pharm Co., Ltd., Seoul, KoreaNon-Clinical Evaluation Center, Biomedical Research Institute, Jeonbuk National University Hospital, Jeonju, KoreaMedical Research Institute of Pusan National University Hospital, Busan, KoreaResearch Institute of Clinical Medicine of Jeonbuk National University-Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, KoreaPurpose: Benign prostatic hyperplasia (BPH) is a urinary tract disorder that primarily affects geriatric males. Natural materials have been developed to treat and prevent symptoms of BPH. However, a few natural functional foods has been conclusively proven to cure or prevent symptoms of BPH. The study aimed to investigate the anti-proliferative mechanism of Curcumae Radix (CR) and Syzygium aromaticum (SA) extracts using RWPE-1 cells and testosterone propionate (TP)-induced BPH rats. Materials and Methods: In vitro experiments were performed to assess the synergistic anti-proliferative effects of an equal mixture of CR and SA extracts on TP-treated RWPE-1 cells. In animal experiments, TP-induced BPH rats were administrated with saline, CR and SA extracts at 50, 100, and 200 mg/kg or finasteride at 1 mg/kg daily for 6 weeks. Body weight, prostate weight, dihydrotestosterone (DHT), androgen receptor (AR), and prostate-specific antigen (PSA) levels were measured. Additionally, extracellular signal-regulated kinase and NF-κB levels, oxidative stress, and inflammatory stress responses in the prostate were also analyzed. Results: In this study, the combination of CR and SA extracts synergistically inhibited cell proliferation compared with the effect of each extract in TP-treated RWPE-1 cells. CR and SA extracts inhibited increasing of prostate weight, thickness of prostate epithelium, the level of PSA and DHT in serum. The expression of protein and gene of PSA and AR in prostate of TPinduced BPH rats were also suppressed by the administration of CR and SA extracts. Furthermore, reactive oxygen species and inflammation axis, NOX4-iNOS-COX2 and its associated representative inflammatory cytokine, interleukin-8 were also reduced in the CR and SA extracts-administered BPH rats. Conclusions: The results suggest that the combination of CR and SA extracts improves BPH through its anti-inflammatory and anti-oxidative effects, demonstrating great potential as an anti-BPH medicine.androgen receptorscurcumaprostate-specific antigenprostatic hyperplasiasyzygium aromaticumtestosterone propionate
spellingShingle Geum Hwa Lee
Yu Seob Shin
Ji Hyun Kim
Da hee Shim
Hwa Young Lee
Luo Zhao
Mohammad Mamun Ur Rashid
Hwang Eui Cho
Jong Jin Lee
Min Kyoung Kim
Do Sung Kim
Hyun Jun Park
Han Jung Chae
The Combination of Curcumae Radix and Syzygium Aromaticum Extracts Mitigates Benign Prostatic Hyperplasia through Anti-Proliferative and Anti-Inflammatory Effects
The World Journal of Men's Health
androgen receptors
curcuma
prostate-specific antigen
prostatic hyperplasia
syzygium aromaticum
testosterone propionate
title The Combination of Curcumae Radix and Syzygium Aromaticum Extracts Mitigates Benign Prostatic Hyperplasia through Anti-Proliferative and Anti-Inflammatory Effects
title_full The Combination of Curcumae Radix and Syzygium Aromaticum Extracts Mitigates Benign Prostatic Hyperplasia through Anti-Proliferative and Anti-Inflammatory Effects
title_fullStr The Combination of Curcumae Radix and Syzygium Aromaticum Extracts Mitigates Benign Prostatic Hyperplasia through Anti-Proliferative and Anti-Inflammatory Effects
title_full_unstemmed The Combination of Curcumae Radix and Syzygium Aromaticum Extracts Mitigates Benign Prostatic Hyperplasia through Anti-Proliferative and Anti-Inflammatory Effects
title_short The Combination of Curcumae Radix and Syzygium Aromaticum Extracts Mitigates Benign Prostatic Hyperplasia through Anti-Proliferative and Anti-Inflammatory Effects
title_sort combination of curcumae radix and syzygium aromaticum extracts mitigates benign prostatic hyperplasia through anti proliferative and anti inflammatory effects
topic androgen receptors
curcuma
prostate-specific antigen
prostatic hyperplasia
syzygium aromaticum
testosterone propionate
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