Higher glass transition temperatures reduce thermal stress cracking in aqueous solutions relevant to cryopreservation
Abstract Cryopreservation by vitrification could transform fields ranging from organ transplantation to wildlife conservation, but critical physical challenges remain in scaling this approach from microscopic to macroscopic systems, including the threat of fracture due to accumulated thermal stresse...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-13295-7 |
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| author | Soheil Kavian Ronald Sellers Gabriel Arismendi Sanchez Crysthal Alvarez Guillermo Aguilar Matthew J. Powell-Palm |
| author_facet | Soheil Kavian Ronald Sellers Gabriel Arismendi Sanchez Crysthal Alvarez Guillermo Aguilar Matthew J. Powell-Palm |
| author_sort | Soheil Kavian |
| collection | DOAJ |
| description | Abstract Cryopreservation by vitrification could transform fields ranging from organ transplantation to wildlife conservation, but critical physical challenges remain in scaling this approach from microscopic to macroscopic systems, including the threat of fracture due to accumulated thermal stresses. Here, we provide experimental and computational evidence that these stresses are strongly dependent on the glass transition temperature $$\:{T}_{g}$$ of the vitrification solution, a property which, given the narrow band of chemistries represented within common vitrification solutions, is seldom investigated in thermomechanical analyses. We develop a custom cryomacroscope platform to image glass cracking in four aqueous solution chemistries spanning > 50 °C in $$\:{T}_{g}$$ ; we process these images using semantic segmentation deep learning algorithms to analyze the extent of cracking in each; and we perform thermomechanical finite element simulations to disentangle the multiphysics effects driving the observed dependency, providing new insights to inform design of next-generation vitrification solutions that minimize thermal cracking risks. |
| format | Article |
| id | doaj-art-c8cfbe2552f347c096a70203efa552ea |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-c8cfbe2552f347c096a70203efa552ea2025-08-20T03:42:25ZengNature PortfolioScientific Reports2045-23222025-07-0115111010.1038/s41598-025-13295-7Higher glass transition temperatures reduce thermal stress cracking in aqueous solutions relevant to cryopreservationSoheil Kavian0Ronald Sellers1Gabriel Arismendi Sanchez2Crysthal Alvarez3Guillermo Aguilar4Matthew J. Powell-Palm5Department of Mechanical Engineering, Texas A&M UniversityDepartment of Mechanical Engineering, Texas A&M UniversityDepartment of Mechanical Engineering, Texas A&M UniversityDepartment of Mechanical Engineering, Texas A&M UniversityDepartment of Mechanical Engineering, Texas A&M UniversityDepartment of Mechanical Engineering, Texas A&M UniversityAbstract Cryopreservation by vitrification could transform fields ranging from organ transplantation to wildlife conservation, but critical physical challenges remain in scaling this approach from microscopic to macroscopic systems, including the threat of fracture due to accumulated thermal stresses. Here, we provide experimental and computational evidence that these stresses are strongly dependent on the glass transition temperature $$\:{T}_{g}$$ of the vitrification solution, a property which, given the narrow band of chemistries represented within common vitrification solutions, is seldom investigated in thermomechanical analyses. We develop a custom cryomacroscope platform to image glass cracking in four aqueous solution chemistries spanning > 50 °C in $$\:{T}_{g}$$ ; we process these images using semantic segmentation deep learning algorithms to analyze the extent of cracking in each; and we perform thermomechanical finite element simulations to disentangle the multiphysics effects driving the observed dependency, providing new insights to inform design of next-generation vitrification solutions that minimize thermal cracking risks.https://doi.org/10.1038/s41598-025-13295-7Glass transitionPhase changeThermal stressVitrification |
| spellingShingle | Soheil Kavian Ronald Sellers Gabriel Arismendi Sanchez Crysthal Alvarez Guillermo Aguilar Matthew J. Powell-Palm Higher glass transition temperatures reduce thermal stress cracking in aqueous solutions relevant to cryopreservation Scientific Reports Glass transition Phase change Thermal stress Vitrification |
| title | Higher glass transition temperatures reduce thermal stress cracking in aqueous solutions relevant to cryopreservation |
| title_full | Higher glass transition temperatures reduce thermal stress cracking in aqueous solutions relevant to cryopreservation |
| title_fullStr | Higher glass transition temperatures reduce thermal stress cracking in aqueous solutions relevant to cryopreservation |
| title_full_unstemmed | Higher glass transition temperatures reduce thermal stress cracking in aqueous solutions relevant to cryopreservation |
| title_short | Higher glass transition temperatures reduce thermal stress cracking in aqueous solutions relevant to cryopreservation |
| title_sort | higher glass transition temperatures reduce thermal stress cracking in aqueous solutions relevant to cryopreservation |
| topic | Glass transition Phase change Thermal stress Vitrification |
| url | https://doi.org/10.1038/s41598-025-13295-7 |
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