New Sensitive Kinetic Spectrophotometric Methods for Determination of Omeprazole in Dosage Forms
New rapid, sensitive, and accurate kinetic spectrophotometric methods were developed, for the first time, to determine omeprazole (OMZ) in its dosage forms. The methods were based on the formation of charge-transfer complexes with both iodine and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ). The...
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Wiley
2009-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2009/307045 |
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| author | Ashraf M. Mahmoud |
| author_facet | Ashraf M. Mahmoud |
| author_sort | Ashraf M. Mahmoud |
| collection | DOAJ |
| description | New rapid, sensitive, and accurate kinetic spectrophotometric methods were developed, for the first time, to determine omeprazole (OMZ) in its dosage forms. The methods were based on the formation of charge-transfer complexes with both iodine and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ). The variables that affected the reactions were carefully studied and optimized. The formed complexes and the site of interaction were examined by UV/VIS, IR, and 1H-NMR techniques, and computational molecular modeling. Under optimum conditions, the stoichiometry of the reactions between OMZ and the acceptors was found to be 1 : 1. The order of the reactions and the specific rate constants were determined. The thermodynamics of the complexes were computed and the mechanism of the reactions was postulated. The initial rate and fixed time methods were utilized for the determination of OMZ concentrations. The linear ranges for the proposed methods were 0.10–3.00 and 0.50–25.00 𝜇g mL−1 with the lowest LOD of 0.03 and 0.14 𝜇g mL−1 for iodine and DDQ, respectively. Analytical performance of the methods was statistically validated; RSD was <1.25% for the precision and <1.95% for the accuracy. The proposed methods were successfully applied to the analysis of OMZ in its dosage forms; the recovery was 98.91–100.32% ± 0.94–1.84, and was found to be comparable with that of reference method. |
| format | Article |
| id | doaj-art-c8cd6819bcb241f381e3627465ff697b |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-c8cd6819bcb241f381e3627465ff697b2025-08-20T03:24:08ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792009-01-01200910.1155/2009/307045307045New Sensitive Kinetic Spectrophotometric Methods for Determination of Omeprazole in Dosage FormsAshraf M. Mahmoud0Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71256, EgyptNew rapid, sensitive, and accurate kinetic spectrophotometric methods were developed, for the first time, to determine omeprazole (OMZ) in its dosage forms. The methods were based on the formation of charge-transfer complexes with both iodine and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ). The variables that affected the reactions were carefully studied and optimized. The formed complexes and the site of interaction were examined by UV/VIS, IR, and 1H-NMR techniques, and computational molecular modeling. Under optimum conditions, the stoichiometry of the reactions between OMZ and the acceptors was found to be 1 : 1. The order of the reactions and the specific rate constants were determined. The thermodynamics of the complexes were computed and the mechanism of the reactions was postulated. The initial rate and fixed time methods were utilized for the determination of OMZ concentrations. The linear ranges for the proposed methods were 0.10–3.00 and 0.50–25.00 𝜇g mL−1 with the lowest LOD of 0.03 and 0.14 𝜇g mL−1 for iodine and DDQ, respectively. Analytical performance of the methods was statistically validated; RSD was <1.25% for the precision and <1.95% for the accuracy. The proposed methods were successfully applied to the analysis of OMZ in its dosage forms; the recovery was 98.91–100.32% ± 0.94–1.84, and was found to be comparable with that of reference method.http://dx.doi.org/10.1155/2009/307045 |
| spellingShingle | Ashraf M. Mahmoud New Sensitive Kinetic Spectrophotometric Methods for Determination of Omeprazole in Dosage Forms International Journal of Analytical Chemistry |
| title | New Sensitive Kinetic Spectrophotometric Methods for Determination of Omeprazole in Dosage Forms |
| title_full | New Sensitive Kinetic Spectrophotometric Methods for Determination of Omeprazole in Dosage Forms |
| title_fullStr | New Sensitive Kinetic Spectrophotometric Methods for Determination of Omeprazole in Dosage Forms |
| title_full_unstemmed | New Sensitive Kinetic Spectrophotometric Methods for Determination of Omeprazole in Dosage Forms |
| title_short | New Sensitive Kinetic Spectrophotometric Methods for Determination of Omeprazole in Dosage Forms |
| title_sort | new sensitive kinetic spectrophotometric methods for determination of omeprazole in dosage forms |
| url | http://dx.doi.org/10.1155/2009/307045 |
| work_keys_str_mv | AT ashrafmmahmoud newsensitivekineticspectrophotometricmethodsfordeterminationofomeprazoleindosageforms |