Directional and Strain-Specific Interaction Between <i>Lactobacillus plantarum</i> and <i>Staphylococcus aureus</i>
The interaction between <i>Lactobacillus plantarum</i> and <i>Staphylococcus aureus</i> strains FRI-1169 and MN8, two original isolated strains from menstrual toxic shock syndrome (mTSS) cases, is a key focus for developing non-antibiotic strategies to control <i>S. aur...
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2024-11-01
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| author | Sandeep Kondakala Sunghyun Yoon Soumana Daddy Gaoh Ohgew Kweon Seong-Jae Kim Mark E. Hart |
| author_facet | Sandeep Kondakala Sunghyun Yoon Soumana Daddy Gaoh Ohgew Kweon Seong-Jae Kim Mark E. Hart |
| author_sort | Sandeep Kondakala |
| collection | DOAJ |
| description | The interaction between <i>Lactobacillus plantarum</i> and <i>Staphylococcus aureus</i> strains FRI-1169 and MN8, two original isolated strains from menstrual toxic shock syndrome (mTSS) cases, is a key focus for developing non-antibiotic strategies to control <i>S. aureus</i>-related infections. While the antagonistic effects of <i>Lactobacilli</i> species on <i>S. aureus</i> through mechanisms like organic acid and bacteriocin production are known, the molecular dynamics of these interactions remain underexplored. This study employs a proteomic approach to analyze the interactions between <i>L. plantarum</i> WCFS1 and <i>S. aureus</i> strains, FRI-1169 and MN8, during co-culture. We profiled differentially expressed proteins (DEPs) found in the spent media and cytosols of both bacteria, revealing distinct directional and strain-specific responses. The findings demonstrate that <i>L. plantarum</i> exerts a more pronounced effect on <i>S. aureus</i>, with more DEPs and upregulated proteins, while <i>S. aureus</i> showed fewer DEPs and more downregulated proteins. These strain-specific interactions highlight the complex metabolic and regulatory adjustments between these bacterial species. This research provides valuable insights into the molecular mechanisms of <i>Lactobacillus-S. aureus</i> antagonism and underscores the potential of proteomic analysis as a powerful tool for studying bacterial dynamics in co-culture systems. |
| format | Article |
| id | doaj-art-c8c9c4932d8b4b71acde5f2951d1b02d |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-c8c9c4932d8b4b71acde5f2951d1b02d2024-12-27T14:41:08ZengMDPI AGMicroorganisms2076-26072024-11-011212243210.3390/microorganisms12122432Directional and Strain-Specific Interaction Between <i>Lactobacillus plantarum</i> and <i>Staphylococcus aureus</i>Sandeep Kondakala0Sunghyun Yoon1Soumana Daddy Gaoh2Ohgew Kweon3Seong-Jae Kim4Mark E. Hart5Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USADivision of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USADivision of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USADivision of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USADivision of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USADivision of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USAThe interaction between <i>Lactobacillus plantarum</i> and <i>Staphylococcus aureus</i> strains FRI-1169 and MN8, two original isolated strains from menstrual toxic shock syndrome (mTSS) cases, is a key focus for developing non-antibiotic strategies to control <i>S. aureus</i>-related infections. While the antagonistic effects of <i>Lactobacilli</i> species on <i>S. aureus</i> through mechanisms like organic acid and bacteriocin production are known, the molecular dynamics of these interactions remain underexplored. This study employs a proteomic approach to analyze the interactions between <i>L. plantarum</i> WCFS1 and <i>S. aureus</i> strains, FRI-1169 and MN8, during co-culture. We profiled differentially expressed proteins (DEPs) found in the spent media and cytosols of both bacteria, revealing distinct directional and strain-specific responses. The findings demonstrate that <i>L. plantarum</i> exerts a more pronounced effect on <i>S. aureus</i>, with more DEPs and upregulated proteins, while <i>S. aureus</i> showed fewer DEPs and more downregulated proteins. These strain-specific interactions highlight the complex metabolic and regulatory adjustments between these bacterial species. This research provides valuable insights into the molecular mechanisms of <i>Lactobacillus-S. aureus</i> antagonism and underscores the potential of proteomic analysis as a powerful tool for studying bacterial dynamics in co-culture systems.https://www.mdpi.com/2076-2607/12/12/2432antagonism<i>Lactobacillus plantarum</i>non-antibiotic biocontrol agentproteomics<i>Staphylococcus aureus</i> infection |
| spellingShingle | Sandeep Kondakala Sunghyun Yoon Soumana Daddy Gaoh Ohgew Kweon Seong-Jae Kim Mark E. Hart Directional and Strain-Specific Interaction Between <i>Lactobacillus plantarum</i> and <i>Staphylococcus aureus</i> Microorganisms antagonism <i>Lactobacillus plantarum</i> non-antibiotic biocontrol agent proteomics <i>Staphylococcus aureus</i> infection |
| title | Directional and Strain-Specific Interaction Between <i>Lactobacillus plantarum</i> and <i>Staphylococcus aureus</i> |
| title_full | Directional and Strain-Specific Interaction Between <i>Lactobacillus plantarum</i> and <i>Staphylococcus aureus</i> |
| title_fullStr | Directional and Strain-Specific Interaction Between <i>Lactobacillus plantarum</i> and <i>Staphylococcus aureus</i> |
| title_full_unstemmed | Directional and Strain-Specific Interaction Between <i>Lactobacillus plantarum</i> and <i>Staphylococcus aureus</i> |
| title_short | Directional and Strain-Specific Interaction Between <i>Lactobacillus plantarum</i> and <i>Staphylococcus aureus</i> |
| title_sort | directional and strain specific interaction between i lactobacillus plantarum i and i staphylococcus aureus i |
| topic | antagonism <i>Lactobacillus plantarum</i> non-antibiotic biocontrol agent proteomics <i>Staphylococcus aureus</i> infection |
| url | https://www.mdpi.com/2076-2607/12/12/2432 |
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