The Supplementation of Cinnamaldehyde in Semen Extender to Improve the Post‐Thaw Sperm Quality of Kajli Ram Semen

ABSTRACT Cinnamaldehyde, a component of cinnamon bark extract, acts as a potential antioxidant by enhancing the activities of catalase, superoxide dismutase and glutathione peroxidase in various cell and animal models. This study aimed to evaluate the protective effects of cinnamaldehyde on the qual...

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Main Authors: Saima Qadeer, Asma Ashraf, Naila Perveen, Muhammad Asad, Asma Ul Husna, Asima Azam, Muhammad Umer Farooq, Abdullah S. Alhomida, Haseeb A. Khan
Format: Article
Language:English
Published: Wiley 2025-07-01
Series:Veterinary Medicine and Science
Subjects:
Online Access:https://doi.org/10.1002/vms3.70484
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author Saima Qadeer
Asma Ashraf
Naila Perveen
Muhammad Asad
Asma Ul Husna
Asima Azam
Muhammad Umer Farooq
Abdullah S. Alhomida
Haseeb A. Khan
author_facet Saima Qadeer
Asma Ashraf
Naila Perveen
Muhammad Asad
Asma Ul Husna
Asima Azam
Muhammad Umer Farooq
Abdullah S. Alhomida
Haseeb A. Khan
author_sort Saima Qadeer
collection DOAJ
description ABSTRACT Cinnamaldehyde, a component of cinnamon bark extract, acts as a potential antioxidant by enhancing the activities of catalase, superoxide dismutase and glutathione peroxidase in various cell and animal models. This study aimed to evaluate the protective effects of cinnamaldehyde on the quality of Kajli ram sperm at 4°C and after freezing. Semen samples were obtained from 12 Kajli rams through an artificial vagina maintained at 42°C. Each ejaculate was divided into five aliquots and extended in a tris‐citric acid extender with different concentrations of cinnamaldehyde (0%, 0.5%, 1.0%, 2.5% and 5.0%). Post‐thaw semen quality was assessed. These results showed that the extender containing 0.5% cinnamaldehyde exhibited significant protective effects (p < 0.05) on post‐thaw sperm plasma membrane and sperm viability compared to the control and other experimental groups. In conclusion, supplementing the extender with 0.5% cinnamaldehyde effectively preserved the quality of post‐thawed sperm from Kajli rams.
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issn 2053-1095
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publishDate 2025-07-01
publisher Wiley
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spelling doaj-art-c8bbd8bb20a64a08bd31be7fa99a20fb2025-08-20T03:55:59ZengWileyVeterinary Medicine and Science2053-10952025-07-01114n/an/a10.1002/vms3.70484The Supplementation of Cinnamaldehyde in Semen Extender to Improve the Post‐Thaw Sperm Quality of Kajli Ram SemenSaima Qadeer0Asma Ashraf1Naila Perveen2Muhammad Asad3Asma Ul Husna4Asima Azam5Muhammad Umer Farooq6Abdullah S. Alhomida7Haseeb A. Khan8Department of Zoology Division of Science and Technology University of Education Lahore PakistanDepartment of Zoology Division of Science and Technology University of Education Lahore PakistanDepartment of Zoology Division of Science and Technology University of Education Lahore PakistanDepartment of Zoology Division of Science and Technology University of Education Lahore PakistanDepartment of Biology University of Haripur Haripur PakistanDepartment of Zoology Shaheed Benazir Bhutto Women University Peshawar PakistanDepartment of Zoology Division of Science and Technology University of Education Lahore PakistanDepartment of Biochemistry College of Science King Saud University Riyadh Saudi ArabiaDepartment of Biochemistry College of Science King Saud University Riyadh Saudi ArabiaABSTRACT Cinnamaldehyde, a component of cinnamon bark extract, acts as a potential antioxidant by enhancing the activities of catalase, superoxide dismutase and glutathione peroxidase in various cell and animal models. This study aimed to evaluate the protective effects of cinnamaldehyde on the quality of Kajli ram sperm at 4°C and after freezing. Semen samples were obtained from 12 Kajli rams through an artificial vagina maintained at 42°C. Each ejaculate was divided into five aliquots and extended in a tris‐citric acid extender with different concentrations of cinnamaldehyde (0%, 0.5%, 1.0%, 2.5% and 5.0%). Post‐thaw semen quality was assessed. These results showed that the extender containing 0.5% cinnamaldehyde exhibited significant protective effects (p < 0.05) on post‐thaw sperm plasma membrane and sperm viability compared to the control and other experimental groups. In conclusion, supplementing the extender with 0.5% cinnamaldehyde effectively preserved the quality of post‐thawed sperm from Kajli rams.https://doi.org/10.1002/vms3.70484antioxidantcinnamaldehydecryopreservationKajli ramsemen
spellingShingle Saima Qadeer
Asma Ashraf
Naila Perveen
Muhammad Asad
Asma Ul Husna
Asima Azam
Muhammad Umer Farooq
Abdullah S. Alhomida
Haseeb A. Khan
The Supplementation of Cinnamaldehyde in Semen Extender to Improve the Post‐Thaw Sperm Quality of Kajli Ram Semen
Veterinary Medicine and Science
antioxidant
cinnamaldehyde
cryopreservation
Kajli ram
semen
title The Supplementation of Cinnamaldehyde in Semen Extender to Improve the Post‐Thaw Sperm Quality of Kajli Ram Semen
title_full The Supplementation of Cinnamaldehyde in Semen Extender to Improve the Post‐Thaw Sperm Quality of Kajli Ram Semen
title_fullStr The Supplementation of Cinnamaldehyde in Semen Extender to Improve the Post‐Thaw Sperm Quality of Kajli Ram Semen
title_full_unstemmed The Supplementation of Cinnamaldehyde in Semen Extender to Improve the Post‐Thaw Sperm Quality of Kajli Ram Semen
title_short The Supplementation of Cinnamaldehyde in Semen Extender to Improve the Post‐Thaw Sperm Quality of Kajli Ram Semen
title_sort supplementation of cinnamaldehyde in semen extender to improve the post thaw sperm quality of kajli ram semen
topic antioxidant
cinnamaldehyde
cryopreservation
Kajli ram
semen
url https://doi.org/10.1002/vms3.70484
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