Effects of the storage of breast milk at different temperatures on total antioxidant capacity, total oxidant status, and paraoxonase-1 level

Breast milk is a well-balanced ideal nutritional source with high bioavailability for infants. As being a fresh, biological and dynamic product, changes in the breast milk during these storage periods have been the subject of ongoing research. This study aims to evaluate total antioxidant capacity...

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Main Authors: Murat Konak, Murat Minici, Nuriye Tarakçı, Hüseyin Altunhan, Aysun Toker, Rahmi Örs
Format: Article
Language:English
Published: Hacettepe University Institute of Child Health 2019-02-01
Series:The Turkish Journal of Pediatrics
Subjects:
Online Access:https://turkjpediatr.org/article/view/643
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author Murat Konak
Murat Minici
Nuriye Tarakçı
Hüseyin Altunhan
Aysun Toker
Rahmi Örs
author_facet Murat Konak
Murat Minici
Nuriye Tarakçı
Hüseyin Altunhan
Aysun Toker
Rahmi Örs
author_sort Murat Konak
collection DOAJ
description Breast milk is a well-balanced ideal nutritional source with high bioavailability for infants. As being a fresh, biological and dynamic product, changes in the breast milk during these storage periods have been the subject of ongoing research. This study aims to evaluate total antioxidant capacity (TAC), total oxidant status (TOS), and paraoxonase-1 (PON-1) levels of fresh and freezestored breast milk. Ten cc of breast milk was obtained from the mothers as the days between 10 and 15 in the morning within a 1-hour period. TAC, TOS, and PON-1 levels were evaluated in the fresh breast milk. Collected breast milk samples were divided into two groups for storage at -20°C or -80°C. Stored samples were tested for TAC, TOS, and PON-1 levels after 72 hours. The highest TAC level was detected in fresh breast milk (p < 0.05). The TOS levels of fresh breast milk showed a statistically significant reduction in rate after storage. The TOS levels at -20°C and -80°C were significantly lower at -80°C (p < 0.05). Our study results show that oxidant and antioxidant activities are at the maximum level in the fresh breast milk. In terms of antioxidant status the effect of freezing temperatures hasn`t been determined. We conclude that it is more convenient to store the breast milk at -80°C than to store at -20°C in terms of preserving the storage TOS level.
format Article
id doaj-art-c8bad84a67184fb68b21dce72ff2afa9
institution OA Journals
issn 0041-4301
2791-6421
language English
publishDate 2019-02-01
publisher Hacettepe University Institute of Child Health
record_format Article
series The Turkish Journal of Pediatrics
spelling doaj-art-c8bad84a67184fb68b21dce72ff2afa92025-08-20T02:01:47ZengHacettepe University Institute of Child HealthThe Turkish Journal of Pediatrics0041-43012791-64212019-02-0161110.24953/turkjped.2019.01.001Effects of the storage of breast milk at different temperatures on total antioxidant capacity, total oxidant status, and paraoxonase-1 levelMurat Konak0Murat Minici1Nuriye Tarakçı2Hüseyin Altunhan3Aysun Toker4Rahmi Örs5Division of Neonatology, Department of Pediatrics, Selçuk University, Selçuklu Medical Faculty, Konya, Turkey.Departments of Pediatrics, Meram Medical Faculty, Necmettin Erbakan University, Konya, Turkey.Departments of Neonatology, Meram Medical Faculty, Necmettin Erbakan University, Konya, Turkey.Departments of Neonatology, Meram Medical Faculty, Necmettin Erbakan University, Konya, Turkey.Departments of Biochemistry, Meram Medical Faculty, Necmettin Erbakan University, Konya, Turkey.Departments of Neonatology, Meram Medical Faculty, Necmettin Erbakan University, Konya, Turkey. Breast milk is a well-balanced ideal nutritional source with high bioavailability for infants. As being a fresh, biological and dynamic product, changes in the breast milk during these storage periods have been the subject of ongoing research. This study aims to evaluate total antioxidant capacity (TAC), total oxidant status (TOS), and paraoxonase-1 (PON-1) levels of fresh and freezestored breast milk. Ten cc of breast milk was obtained from the mothers as the days between 10 and 15 in the morning within a 1-hour period. TAC, TOS, and PON-1 levels were evaluated in the fresh breast milk. Collected breast milk samples were divided into two groups for storage at -20°C or -80°C. Stored samples were tested for TAC, TOS, and PON-1 levels after 72 hours. The highest TAC level was detected in fresh breast milk (p < 0.05). The TOS levels of fresh breast milk showed a statistically significant reduction in rate after storage. The TOS levels at -20°C and -80°C were significantly lower at -80°C (p < 0.05). Our study results show that oxidant and antioxidant activities are at the maximum level in the fresh breast milk. In terms of antioxidant status the effect of freezing temperatures hasn`t been determined. We conclude that it is more convenient to store the breast milk at -80°C than to store at -20°C in terms of preserving the storage TOS level. https://turkjpediatr.org/article/view/643antioxidant statusoxidant statusparaoxonase-1stored breast milk
spellingShingle Murat Konak
Murat Minici
Nuriye Tarakçı
Hüseyin Altunhan
Aysun Toker
Rahmi Örs
Effects of the storage of breast milk at different temperatures on total antioxidant capacity, total oxidant status, and paraoxonase-1 level
The Turkish Journal of Pediatrics
antioxidant status
oxidant status
paraoxonase-1
stored breast milk
title Effects of the storage of breast milk at different temperatures on total antioxidant capacity, total oxidant status, and paraoxonase-1 level
title_full Effects of the storage of breast milk at different temperatures on total antioxidant capacity, total oxidant status, and paraoxonase-1 level
title_fullStr Effects of the storage of breast milk at different temperatures on total antioxidant capacity, total oxidant status, and paraoxonase-1 level
title_full_unstemmed Effects of the storage of breast milk at different temperatures on total antioxidant capacity, total oxidant status, and paraoxonase-1 level
title_short Effects of the storage of breast milk at different temperatures on total antioxidant capacity, total oxidant status, and paraoxonase-1 level
title_sort effects of the storage of breast milk at different temperatures on total antioxidant capacity total oxidant status and paraoxonase 1 level
topic antioxidant status
oxidant status
paraoxonase-1
stored breast milk
url https://turkjpediatr.org/article/view/643
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