Intestine‐Decipher Engineered Capsules Protect Against Sepsis‐induced Intestinal Injury via Broad‐spectrum Anti‐inflammation and Parthanatos Inhibition

Abstract Sepsis is a severe systemic inflammatory syndrome characterized by a dysregulated immune response to infection, often leading to high mortality rates. The intestine, owing to its distinct structure and physiological environment, plays a pivotal role in the pathophysiology of sepsis. It func...

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Main Authors: Yan Yan, Bin Li, Qiuxia Gao, Miao Wu, Hua Ma, Jiawei Bai, Chengtai Ma, Xinyu Xie, Yuan Gong, Lingqi Xu, Xiaoxue Li, Wei Wang, Yanqiu Wu, Jiamei Wang, Huanhuan Wang, Yi Feng, Yunlong Zhang, Peiran Li, Huimin Shi, Fei Ma, Yue Jia, Han Duan, Xinting Fu, Wenying Wang, Liying Zhan, Xianjin Du, Huiting Zhou, Yuhui Liao
Format: Article
Language:English
Published: Wiley 2025-03-01
Series:Advanced Science
Subjects:
Online Access:https://doi.org/10.1002/advs.202412799
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author Yan Yan
Bin Li
Qiuxia Gao
Miao Wu
Hua Ma
Jiawei Bai
Chengtai Ma
Xinyu Xie
Yuan Gong
Lingqi Xu
Xiaoxue Li
Wei Wang
Yanqiu Wu
Jiamei Wang
Huanhuan Wang
Yi Feng
Yunlong Zhang
Peiran Li
Huimin Shi
Fei Ma
Yue Jia
Han Duan
Xinting Fu
Wenying Wang
Liying Zhan
Xianjin Du
Huiting Zhou
Yuhui Liao
author_facet Yan Yan
Bin Li
Qiuxia Gao
Miao Wu
Hua Ma
Jiawei Bai
Chengtai Ma
Xinyu Xie
Yuan Gong
Lingqi Xu
Xiaoxue Li
Wei Wang
Yanqiu Wu
Jiamei Wang
Huanhuan Wang
Yi Feng
Yunlong Zhang
Peiran Li
Huimin Shi
Fei Ma
Yue Jia
Han Duan
Xinting Fu
Wenying Wang
Liying Zhan
Xianjin Du
Huiting Zhou
Yuhui Liao
author_sort Yan Yan
collection DOAJ
description Abstract Sepsis is a severe systemic inflammatory syndrome characterized by a dysregulated immune response to infection, often leading to high mortality rates. The intestine, owing to its distinct structure and physiological environment, plays a pivotal role in the pathophysiology of sepsis. It functions as the “central organ” or “engine” in the progression of sepsis, with intestinal injury exacerbating the condition. Despite the availability of current therapies that offer partial symptom relief, they fall short of adequately protecting the intestinal barrier. In this study, an advanced nanodrug formulation (OLA@MΦ NPs) is developed by coating macrophage membranes onto polymeric organic nanoparticles encapsulating olaparib. When loaded into pH‐responsive capsules, an intestine‐decipher engineered capsule (cp‐OLA@MΦ NPs) is successfully formulated. Upon oral administration in septic mice, these capsules withstand gastric acid and release their contents in the intestine, specifically targeting injured tissues. The released OLA@MΦ NPs effectively neutralize pro‐inflammatory cytokines via macrophage membrane receptors, while olaparib inhibits intestinal epithelial parthanatos (a form of programmed cell death) by suppressing poly(ADP‐ribose) polymerase 1 (PARP1) activation. This strategy significantly reduces bacterial translocation, slows the progression of sepsis, and enhances survival in septic mice, thus presenting a promising therapeutic approach for sepsis in clinical applications.
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spelling doaj-art-c8af76284ec84178812db5c35ddf72ed2025-08-20T02:35:40ZengWileyAdvanced Science2198-38442025-03-011210n/an/a10.1002/advs.202412799Intestine‐Decipher Engineered Capsules Protect Against Sepsis‐induced Intestinal Injury via Broad‐spectrum Anti‐inflammation and Parthanatos InhibitionYan Yan0Bin Li1Qiuxia Gao2Miao Wu3Hua Ma4Jiawei Bai5Chengtai Ma6Xinyu Xie7Yuan Gong8Lingqi Xu9Xiaoxue Li10Wei Wang11Yanqiu Wu12Jiamei Wang13Huanhuan Wang14Yi Feng15Yunlong Zhang16Peiran Li17Huimin Shi18Fei Ma19Yue Jia20Han Duan21Xinting Fu22Wenying Wang23Liying Zhan24Xianjin Du25Huiting Zhou26Yuhui Liao27Department of Critical Care Medicine Renmin Hospital of Wuhan University Wuhan 430060 ChinaSchool of Inspection Ningxia Medical University Yinchuan Ningxia 750004 ChinaInstitute for Engineering Medicine Kunming Medical University Kunming 650500 ChinaDepartment of Critical Care Medicine Renmin Hospital of Wuhan University Wuhan 430060 ChinaInstitute for Engineering Medicine Kunming Medical University Kunming 650500 ChinaDepartment of Critical Care Medicine Renmin Hospital of Wuhan University Wuhan 430060 ChinaDepartment of Critical Care Medicine Renmin Hospital of Wuhan University Wuhan 430060 ChinaDepartment of Critical Care Medicine Renmin Hospital of Wuhan University Wuhan 430060 ChinaInstitute of Pediatric Research Children's Hospital of Soochow University Suzhou 215025 ChinaInstitute of Pediatric Research Children's Hospital of Soochow University Suzhou 215025 ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital of Southern Medical University Guangzhou Guangdong 510091 ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital of Southern Medical University Guangzhou Guangdong 510091 ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital of Southern Medical University Guangzhou Guangdong 510091 ChinaInstitute for Engineering Medicine Kunming Medical University Kunming 650500 ChinaInstitute for Engineering Medicine Kunming Medical University Kunming 650500 ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital of Southern Medical University Guangzhou Guangdong 510091 ChinaDepartment of Critical Care Medicine Renmin Hospital of Wuhan University Wuhan 430060 ChinaDepartment of Microbiology School of Public Health Southern Medical University Guangzhou 510515 ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital of Southern Medical University Guangzhou Guangdong 510091 ChinaSchool of Inspection Ningxia Medical University Yinchuan Ningxia 750004 ChinaSchool of Inspection Ningxia Medical University Yinchuan Ningxia 750004 ChinaDepartment of Microbiology School of Public Health Southern Medical University Guangzhou 510515 ChinaDepartment of Microbiology School of Public Health Southern Medical University Guangzhou 510515 ChinaInstitute for Engineering Medicine Kunming Medical University Kunming 650500 ChinaDepartment of Critical Care Medicine Renmin Hospital of Wuhan University Wuhan 430060 ChinaDepartment of Critical Care Medicine Renmin Hospital of Wuhan University Wuhan 430060 ChinaInstitute of Pediatric Research Children's Hospital of Soochow University Suzhou 215025 ChinaSchool of Inspection Ningxia Medical University Yinchuan Ningxia 750004 ChinaAbstract Sepsis is a severe systemic inflammatory syndrome characterized by a dysregulated immune response to infection, often leading to high mortality rates. The intestine, owing to its distinct structure and physiological environment, plays a pivotal role in the pathophysiology of sepsis. It functions as the “central organ” or “engine” in the progression of sepsis, with intestinal injury exacerbating the condition. Despite the availability of current therapies that offer partial symptom relief, they fall short of adequately protecting the intestinal barrier. In this study, an advanced nanodrug formulation (OLA@MΦ NPs) is developed by coating macrophage membranes onto polymeric organic nanoparticles encapsulating olaparib. When loaded into pH‐responsive capsules, an intestine‐decipher engineered capsule (cp‐OLA@MΦ NPs) is successfully formulated. Upon oral administration in septic mice, these capsules withstand gastric acid and release their contents in the intestine, specifically targeting injured tissues. The released OLA@MΦ NPs effectively neutralize pro‐inflammatory cytokines via macrophage membrane receptors, while olaparib inhibits intestinal epithelial parthanatos (a form of programmed cell death) by suppressing poly(ADP‐ribose) polymerase 1 (PARP1) activation. This strategy significantly reduces bacterial translocation, slows the progression of sepsis, and enhances survival in septic mice, thus presenting a promising therapeutic approach for sepsis in clinical applications.https://doi.org/10.1002/advs.202412799broad‐spectrum anti‐inflammationolaparibparthanatospH‐responsive capsulessepsis‐induced intestinal injury
spellingShingle Yan Yan
Bin Li
Qiuxia Gao
Miao Wu
Hua Ma
Jiawei Bai
Chengtai Ma
Xinyu Xie
Yuan Gong
Lingqi Xu
Xiaoxue Li
Wei Wang
Yanqiu Wu
Jiamei Wang
Huanhuan Wang
Yi Feng
Yunlong Zhang
Peiran Li
Huimin Shi
Fei Ma
Yue Jia
Han Duan
Xinting Fu
Wenying Wang
Liying Zhan
Xianjin Du
Huiting Zhou
Yuhui Liao
Intestine‐Decipher Engineered Capsules Protect Against Sepsis‐induced Intestinal Injury via Broad‐spectrum Anti‐inflammation and Parthanatos Inhibition
Advanced Science
broad‐spectrum anti‐inflammation
olaparib
parthanatos
pH‐responsive capsules
sepsis‐induced intestinal injury
title Intestine‐Decipher Engineered Capsules Protect Against Sepsis‐induced Intestinal Injury via Broad‐spectrum Anti‐inflammation and Parthanatos Inhibition
title_full Intestine‐Decipher Engineered Capsules Protect Against Sepsis‐induced Intestinal Injury via Broad‐spectrum Anti‐inflammation and Parthanatos Inhibition
title_fullStr Intestine‐Decipher Engineered Capsules Protect Against Sepsis‐induced Intestinal Injury via Broad‐spectrum Anti‐inflammation and Parthanatos Inhibition
title_full_unstemmed Intestine‐Decipher Engineered Capsules Protect Against Sepsis‐induced Intestinal Injury via Broad‐spectrum Anti‐inflammation and Parthanatos Inhibition
title_short Intestine‐Decipher Engineered Capsules Protect Against Sepsis‐induced Intestinal Injury via Broad‐spectrum Anti‐inflammation and Parthanatos Inhibition
title_sort intestine decipher engineered capsules protect against sepsis induced intestinal injury via broad spectrum anti inflammation and parthanatos inhibition
topic broad‐spectrum anti‐inflammation
olaparib
parthanatos
pH‐responsive capsules
sepsis‐induced intestinal injury
url https://doi.org/10.1002/advs.202412799
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