Optimizing In Vitro Propagation of <i>Haworthia truncata</i> Schönland Using Leaf, Root, and Inflorescence
<i>Haworthia truncata</i>, a species native to South Africa, is characterized by its limited growth and scarcity, contributing to high production costs. Countries like China and Turkey are known for exporting <i>Haworthia</i> globally. Tissue culture offers an efficient metho...
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2025-01-01
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| author | Leila Soleimani Hassan Salehi Taras Pasternak |
| author_facet | Leila Soleimani Hassan Salehi Taras Pasternak |
| author_sort | Leila Soleimani |
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| description | <i>Haworthia truncata</i>, a species native to South Africa, is characterized by its limited growth and scarcity, contributing to high production costs. Countries like China and Turkey are known for exporting <i>Haworthia</i> globally. Tissue culture offers an efficient method for mass-producing unique and beautiful species such as <i>H. truncata.</i> This study tested Murashige and Skoog (MS) basal media supplemented with various concentrations of IBA (0.05–1.5 mg/L), NAA (0.05–0.25 mg/L), and BA (0.25–1.5 mg/L) to promote shoot proliferation. MS medium without plant growth regulators (PGRs) was also tested as a control. Different explant types (leaf, root, and inflorescence) were analyzed for their potential in direct and indirect regeneration. Inflorescence explants showed the highest callus induction with 1.5 mg/L IBA, while optimal shoot proliferation occurred at 1 mg/L IBA. Callus induction was optimal for leaf explants with 0.05 mg/L NAA and 0.25 mg/L BA, and shoot proliferation was highest at 0.05 mg/L NAA and 1 mg/L BA. Root explants achieved maximum callus induction with 0.25 mg/L BA and 0.25 mg/L NAA, with the best shoot proliferation using 0.05 mg/L NAA and 1 mg/L BA. The highest rooting percentage of regenerated shoots was obtained on ½ MS medium with 1.5 mg/L IBA. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-c8a722960a784e148e0f70fa0130cc622025-01-24T13:46:47ZengMDPI AGPlants2223-77472025-01-0114221210.3390/plants14020212Optimizing In Vitro Propagation of <i>Haworthia truncata</i> Schönland Using Leaf, Root, and InflorescenceLeila Soleimani0Hassan Salehi1Taras Pasternak2Department of Horticultural Science, School of Agriculture, Shiraz University, Shiraz 7144113131, IranDepartment of Horticultural Science, School of Agriculture, Shiraz University, Shiraz 7144113131, IranInstituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain<i>Haworthia truncata</i>, a species native to South Africa, is characterized by its limited growth and scarcity, contributing to high production costs. Countries like China and Turkey are known for exporting <i>Haworthia</i> globally. Tissue culture offers an efficient method for mass-producing unique and beautiful species such as <i>H. truncata.</i> This study tested Murashige and Skoog (MS) basal media supplemented with various concentrations of IBA (0.05–1.5 mg/L), NAA (0.05–0.25 mg/L), and BA (0.25–1.5 mg/L) to promote shoot proliferation. MS medium without plant growth regulators (PGRs) was also tested as a control. Different explant types (leaf, root, and inflorescence) were analyzed for their potential in direct and indirect regeneration. Inflorescence explants showed the highest callus induction with 1.5 mg/L IBA, while optimal shoot proliferation occurred at 1 mg/L IBA. Callus induction was optimal for leaf explants with 0.05 mg/L NAA and 0.25 mg/L BA, and shoot proliferation was highest at 0.05 mg/L NAA and 1 mg/L BA. Root explants achieved maximum callus induction with 0.25 mg/L BA and 0.25 mg/L NAA, with the best shoot proliferation using 0.05 mg/L NAA and 1 mg/L BA. The highest rooting percentage of regenerated shoots was obtained on ½ MS medium with 1.5 mg/L IBA.https://www.mdpi.com/2223-7747/14/2/212acclimatizationadventitious shoot regenerationmicropropagationsucculent plantsSEMtissue culture |
| spellingShingle | Leila Soleimani Hassan Salehi Taras Pasternak Optimizing In Vitro Propagation of <i>Haworthia truncata</i> Schönland Using Leaf, Root, and Inflorescence Plants acclimatization adventitious shoot regeneration micropropagation succulent plants SEM tissue culture |
| title | Optimizing In Vitro Propagation of <i>Haworthia truncata</i> Schönland Using Leaf, Root, and Inflorescence |
| title_full | Optimizing In Vitro Propagation of <i>Haworthia truncata</i> Schönland Using Leaf, Root, and Inflorescence |
| title_fullStr | Optimizing In Vitro Propagation of <i>Haworthia truncata</i> Schönland Using Leaf, Root, and Inflorescence |
| title_full_unstemmed | Optimizing In Vitro Propagation of <i>Haworthia truncata</i> Schönland Using Leaf, Root, and Inflorescence |
| title_short | Optimizing In Vitro Propagation of <i>Haworthia truncata</i> Schönland Using Leaf, Root, and Inflorescence |
| title_sort | optimizing in vitro propagation of i haworthia truncata i schonland using leaf root and inflorescence |
| topic | acclimatization adventitious shoot regeneration micropropagation succulent plants SEM tissue culture |
| url | https://www.mdpi.com/2223-7747/14/2/212 |
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