THE ABILITY OF Trichoderma harzianum TO PRODUCE CHITINASE
The result showed that optimum temperature for maximum activity of Chitinase was 25 Cº with 2.74 unit/µm/min. while optimum pH for maximum activity Chitinase was 5 with 2.25 unit/µm/min . Significant activities of chitinase was produced by T. harzianum in culture media amended with fresh mycelium...
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| Language: | English |
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College of Agriculture
2011-12-01
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| Series: | Mesopotamia Journal of Agriculture |
| Online Access: | https://magrj.mosuljournals.com/article_28133_9307587291f0fde42ad71f4d45828b5a.pdf |
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| author | KHALID H.TAHA |
| author_facet | KHALID H.TAHA |
| author_sort | KHALID H.TAHA |
| collection | DOAJ |
| description | The result showed that optimum temperature for maximum activity of Chitinase was 25 Cº with 2.74 unit/µm/min. while optimum pH for maximum activity Chitinase was 5 with 2.25 unit/µm/min . Significant activities of chitinase was produced by T. harzianum in culture media amended with fresh mycelium of M. phaseolina , where the releasing of reducing sugar N-acetylglucosamin was 280 µg/ml which Significantly differ from T. harzianum culture media amended with fresh and dry mycelium of R.solani and F.solani where the releasing of reducing sugar N-acetylglucosamin was 200 µg/ml. Production of chitinase using a medium containing chitin reached 40 µg/ml . Maximium chitinase inhibition activity, purified by ammonium sulphate, was recorded for M. phaseolina mycelium growth 47.03% , which Significantly differ with of R.solani and F.solani where the inhibition activity was 35.18 and 33.33% respectively |
| format | Article |
| id | doaj-art-c8a62ca5002f43ae81e4e2a06e540afb |
| institution | OA Journals |
| issn | 1815-316X 2224-9796 |
| language | English |
| publishDate | 2011-12-01 |
| publisher | College of Agriculture |
| record_format | Article |
| series | Mesopotamia Journal of Agriculture |
| spelling | doaj-art-c8a62ca5002f43ae81e4e2a06e540afb2025-08-20T02:19:40ZengCollege of AgricultureMesopotamia Journal of Agriculture1815-316X2224-97962011-12-0139423724310.33899/magrj.2011.2813328133THE ABILITY OF Trichoderma harzianum TO PRODUCE CHITINASEKHALID H.TAHAThe result showed that optimum temperature for maximum activity of Chitinase was 25 Cº with 2.74 unit/µm/min. while optimum pH for maximum activity Chitinase was 5 with 2.25 unit/µm/min . Significant activities of chitinase was produced by T. harzianum in culture media amended with fresh mycelium of M. phaseolina , where the releasing of reducing sugar N-acetylglucosamin was 280 µg/ml which Significantly differ from T. harzianum culture media amended with fresh and dry mycelium of R.solani and F.solani where the releasing of reducing sugar N-acetylglucosamin was 200 µg/ml. Production of chitinase using a medium containing chitin reached 40 µg/ml . Maximium chitinase inhibition activity, purified by ammonium sulphate, was recorded for M. phaseolina mycelium growth 47.03% , which Significantly differ with of R.solani and F.solani where the inhibition activity was 35.18 and 33.33% respectivelyhttps://magrj.mosuljournals.com/article_28133_9307587291f0fde42ad71f4d45828b5a.pdf |
| spellingShingle | KHALID H.TAHA THE ABILITY OF Trichoderma harzianum TO PRODUCE CHITINASE Mesopotamia Journal of Agriculture |
| title | THE ABILITY OF Trichoderma harzianum TO PRODUCE CHITINASE |
| title_full | THE ABILITY OF Trichoderma harzianum TO PRODUCE CHITINASE |
| title_fullStr | THE ABILITY OF Trichoderma harzianum TO PRODUCE CHITINASE |
| title_full_unstemmed | THE ABILITY OF Trichoderma harzianum TO PRODUCE CHITINASE |
| title_short | THE ABILITY OF Trichoderma harzianum TO PRODUCE CHITINASE |
| title_sort | ability of trichoderma harzianum to produce chitinase |
| url | https://magrj.mosuljournals.com/article_28133_9307587291f0fde42ad71f4d45828b5a.pdf |
| work_keys_str_mv | AT khalidhtaha theabilityoftrichodermaharzianumtoproducechitinase AT khalidhtaha abilityoftrichodermaharzianumtoproducechitinase |