Construction and research of bioreactor simulation scale-up model

Objective: Building a perfect bioreactor scale-up model provides convenience for enterprise production, and a guidance for reactor manufacturers. Methods: Through the test of 1.5, 5, 20, 200 L scale reactor, the correlation and parameters of the volume dissolved oxygen coefficient kLa and mixing tim...

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Main Authors: HU Yi-wen, HAN Fei-fei, JIN Kui-qi, SUN Yang
Format: Article
Language:English
Published: The Editorial Office of Food and Machinery 2023-04-01
Series:Shipin yu jixie
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Online Access:http://www.ifoodmm.com/spyjxen/article/abstract/20230104
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author HU Yi-wen
HAN Fei-fei
JIN Kui-qi
SUN Yang
author_facet HU Yi-wen
HAN Fei-fei
JIN Kui-qi
SUN Yang
author_sort HU Yi-wen
collection DOAJ
description Objective: Building a perfect bioreactor scale-up model provides convenience for enterprise production, and a guidance for reactor manufacturers. Methods: Through the test of 1.5, 5, 20, 200 L scale reactor, the correlation and parameters of the volume dissolved oxygen coefficient kLa and mixing time tm were optimized and explored, the simulation amplification model of bioreactor was established, and the Scaleuper amplification system was built by MATLAB. Results: Compared with the test results, the simulation deviation of kLa was within ±15%. By introducing the definition of critical speed into the correlation of tm, the overall simulation deviation of tm was reduced by 17.4% compared with the original formula. Within ±10%, the simulation deviation of kLa was reduced by embedding BP neural network. The minimum simulation deviation of BP model can reach 0.1% for the training data range. Conclusion: The scale-up model of the reactor based on the empirical correlation has better simulation results after the relevant parameters are measured. It is feasible to use neural network to simulate and amplify the reactor, but it still needs more data training support.
format Article
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institution OA Journals
issn 1003-5788
language English
publishDate 2023-04-01
publisher The Editorial Office of Food and Machinery
record_format Article
series Shipin yu jixie
spelling doaj-art-c8a4b9c237404ce79749045789743d1a2025-08-20T02:22:37ZengThe Editorial Office of Food and MachineryShipin yu jixie1003-57882023-04-01391182310.13652/j.spjx.1003.5788.2022.80403Construction and research of bioreactor simulation scale-up modelHU Yi-wen0HAN Fei-fei1JIN Kui-qi2SUN Yang3 School of Life Sciences, Henan University, Kaifeng, Henan 475004 , China BIO-YD-Pharmaceutical-Since, Chengdu, Sichuan 610105 , China BIO-YD-Pharmaceutical-Since, Chengdu, Sichuan 610105 , China School of Life Sciences, Henan University, Kaifeng, Henan 475004 , China Objective: Building a perfect bioreactor scale-up model provides convenience for enterprise production, and a guidance for reactor manufacturers. Methods: Through the test of 1.5, 5, 20, 200 L scale reactor, the correlation and parameters of the volume dissolved oxygen coefficient kLa and mixing time tm were optimized and explored, the simulation amplification model of bioreactor was established, and the Scaleuper amplification system was built by MATLAB. Results: Compared with the test results, the simulation deviation of kLa was within ±15%. By introducing the definition of critical speed into the correlation of tm, the overall simulation deviation of tm was reduced by 17.4% compared with the original formula. Within ±10%, the simulation deviation of kLa was reduced by embedding BP neural network. The minimum simulation deviation of BP model can reach 0.1% for the training data range. Conclusion: The scale-up model of the reactor based on the empirical correlation has better simulation results after the relevant parameters are measured. It is feasible to use neural network to simulate and amplify the reactor, but it still needs more data training support.http://www.ifoodmm.com/spyjxen/article/abstract/20230104 mixing time volumetric dissolved oxygen coefficient bioreactor scale-up neural network scale-up model
spellingShingle HU Yi-wen
HAN Fei-fei
JIN Kui-qi
SUN Yang
Construction and research of bioreactor simulation scale-up model
Shipin yu jixie
mixing time
volumetric dissolved oxygen coefficient
bioreactor scale-up
neural network
scale-up model
title Construction and research of bioreactor simulation scale-up model
title_full Construction and research of bioreactor simulation scale-up model
title_fullStr Construction and research of bioreactor simulation scale-up model
title_full_unstemmed Construction and research of bioreactor simulation scale-up model
title_short Construction and research of bioreactor simulation scale-up model
title_sort construction and research of bioreactor simulation scale up model
topic mixing time
volumetric dissolved oxygen coefficient
bioreactor scale-up
neural network
scale-up model
url http://www.ifoodmm.com/spyjxen/article/abstract/20230104
work_keys_str_mv AT huyiwen constructionandresearchofbioreactorsimulationscaleupmodel
AT hanfeifei constructionandresearchofbioreactorsimulationscaleupmodel
AT jinkuiqi constructionandresearchofbioreactorsimulationscaleupmodel
AT sunyang constructionandresearchofbioreactorsimulationscaleupmodel