Mouse embryonic kidney transplantation identifies maturation defects in the medulla
Abstract Kidney organoids are connected to the host circulation and mature after transplantation. However, they are still immature compared to the adult kidneys, and their precise maturation stages remain unclear. By transplanting the mouse embryonic kidney as a model system for organoid transplanta...
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| Format: | Article |
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-81984-w |
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| author | Hiroshi Ide Koichiro Miike Tomoko Ohmori Kosuke Maruyama Yuichiro Izumi Shunsuke Tanigawa Ryuichi Nishinakamura |
| author_facet | Hiroshi Ide Koichiro Miike Tomoko Ohmori Kosuke Maruyama Yuichiro Izumi Shunsuke Tanigawa Ryuichi Nishinakamura |
| author_sort | Hiroshi Ide |
| collection | DOAJ |
| description | Abstract Kidney organoids are connected to the host circulation and mature after transplantation. However, they are still immature compared to the adult kidneys, and their precise maturation stages remain unclear. By transplanting the mouse embryonic kidney as a model system for organoid transplantation, we report here the maturation defects of the graft, especially in the medulla. Single cell profiling of the developing kidneys in vivo identified gene sets associated with the maturation of the collecting duct epithelium and medullary stroma. These data revealed an upregulation of genes associated with channel/transporter functions and immune defense, as well as a downregulation of neuronal genes. Using these marker genes, we found that the maturation of the collecting duct and medullary stroma in the grafts barely corresponds to the perinatal stage, which was confirmed histologically by using representative genes. Thus, the gene sets obtained serve as maturation coordinates for the renal medulla and will be helpful in analyzing its maturation defects after transplantation. They will also provide a useful basis for further maturation of transplanted kidney organoids. |
| format | Article |
| id | doaj-art-c8a0016109b946b8911c4804f625e324 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-c8a0016109b946b8911c4804f625e3242025-08-20T02:30:58ZengNature PortfolioScientific Reports2045-23222024-12-0114111310.1038/s41598-024-81984-wMouse embryonic kidney transplantation identifies maturation defects in the medullaHiroshi Ide0Koichiro Miike1Tomoko Ohmori2Kosuke Maruyama3Yuichiro Izumi4Shunsuke Tanigawa5Ryuichi Nishinakamura6Department of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto UniversityDepartment of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto UniversityDepartment of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto UniversityDepartment of Nephrology, Kumamoto University Graduate School of Medical SciencesDepartment of Nephrology, Kumamoto University Graduate School of Medical SciencesDepartment of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto UniversityDepartment of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto UniversityAbstract Kidney organoids are connected to the host circulation and mature after transplantation. However, they are still immature compared to the adult kidneys, and their precise maturation stages remain unclear. By transplanting the mouse embryonic kidney as a model system for organoid transplantation, we report here the maturation defects of the graft, especially in the medulla. Single cell profiling of the developing kidneys in vivo identified gene sets associated with the maturation of the collecting duct epithelium and medullary stroma. These data revealed an upregulation of genes associated with channel/transporter functions and immune defense, as well as a downregulation of neuronal genes. Using these marker genes, we found that the maturation of the collecting duct and medullary stroma in the grafts barely corresponds to the perinatal stage, which was confirmed histologically by using representative genes. Thus, the gene sets obtained serve as maturation coordinates for the renal medulla and will be helpful in analyzing its maturation defects after transplantation. They will also provide a useful basis for further maturation of transplanted kidney organoids.https://doi.org/10.1038/s41598-024-81984-wKidney organoidEmbryonic kidney transplantationRenal medullaMaturationscRNA-seq |
| spellingShingle | Hiroshi Ide Koichiro Miike Tomoko Ohmori Kosuke Maruyama Yuichiro Izumi Shunsuke Tanigawa Ryuichi Nishinakamura Mouse embryonic kidney transplantation identifies maturation defects in the medulla Scientific Reports Kidney organoid Embryonic kidney transplantation Renal medulla Maturation scRNA-seq |
| title | Mouse embryonic kidney transplantation identifies maturation defects in the medulla |
| title_full | Mouse embryonic kidney transplantation identifies maturation defects in the medulla |
| title_fullStr | Mouse embryonic kidney transplantation identifies maturation defects in the medulla |
| title_full_unstemmed | Mouse embryonic kidney transplantation identifies maturation defects in the medulla |
| title_short | Mouse embryonic kidney transplantation identifies maturation defects in the medulla |
| title_sort | mouse embryonic kidney transplantation identifies maturation defects in the medulla |
| topic | Kidney organoid Embryonic kidney transplantation Renal medulla Maturation scRNA-seq |
| url | https://doi.org/10.1038/s41598-024-81984-w |
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