The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells
The goal of this study was to determine and compare the effects of the secretome of mesenchymal stem cells (MSCs) isolated from human bone-marrow (BMSCs) and the Wharton jelly surrounding the vein and arteries of the umbilical cord (human umbilical cord perivascular cells (HUCPVCs)) on the survival...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2014/438352 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560414189158400 |
|---|---|
| author | Ana O. Pires Andreia Neves-Carvalho Nuno Sousa António J. Salgado |
| author_facet | Ana O. Pires Andreia Neves-Carvalho Nuno Sousa António J. Salgado |
| author_sort | Ana O. Pires |
| collection | DOAJ |
| description | The goal of this study was to determine and compare the effects of the secretome of mesenchymal stem cells (MSCs) isolated from human bone-marrow (BMSCs) and the Wharton jelly surrounding the vein and arteries of the umbilical cord (human umbilical cord perivascular cells (HUCPVCs)) on the survival and differentiation of a human neuroblastoma cell line (SH-SY5Y). For this purpose, SH-SY5Y cells were differentiated with conditioned media (CM) from the MSCs populations referred above. Retinoic acid cultured cells were used as control for neuronal differentiated SH-SY5Y cells. SH-SY5Y cells viability assessment revealed that the secretome of BMSCs and HUCPVCs, in the form of CM, was able to induce their survival. Moreover, immunocytochemical experiments showed that CM from both MSCs was capable of inducing neuronal differentiation of SH-SY5Y cells. Finally, neurite lengths assessment and quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) analysis demonstrated that CM from BMSCs and HUCPVCs differently induced neurite outgrowth and mRNA levels of neuronal markers exhibited by SH-SY5Y cells. Overall, our results show that the secretome of both BMSCs and HUCPVCs was capable of supporting SH-SY5Y cells survival and promoting their differentiation towards a neuronal phenotype. |
| format | Article |
| id | doaj-art-c89eae7be9b148e2ab0628dafddc3e42 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-c89eae7be9b148e2ab0628dafddc3e422025-02-03T01:27:41ZengWileyStem Cells International1687-966X1687-96782014-01-01201410.1155/2014/438352438352The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y CellsAna O. Pires0Andreia Neves-Carvalho1Nuno Sousa2António J. Salgado3Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalLife and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalLife and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalLife and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalThe goal of this study was to determine and compare the effects of the secretome of mesenchymal stem cells (MSCs) isolated from human bone-marrow (BMSCs) and the Wharton jelly surrounding the vein and arteries of the umbilical cord (human umbilical cord perivascular cells (HUCPVCs)) on the survival and differentiation of a human neuroblastoma cell line (SH-SY5Y). For this purpose, SH-SY5Y cells were differentiated with conditioned media (CM) from the MSCs populations referred above. Retinoic acid cultured cells were used as control for neuronal differentiated SH-SY5Y cells. SH-SY5Y cells viability assessment revealed that the secretome of BMSCs and HUCPVCs, in the form of CM, was able to induce their survival. Moreover, immunocytochemical experiments showed that CM from both MSCs was capable of inducing neuronal differentiation of SH-SY5Y cells. Finally, neurite lengths assessment and quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) analysis demonstrated that CM from BMSCs and HUCPVCs differently induced neurite outgrowth and mRNA levels of neuronal markers exhibited by SH-SY5Y cells. Overall, our results show that the secretome of both BMSCs and HUCPVCs was capable of supporting SH-SY5Y cells survival and promoting their differentiation towards a neuronal phenotype.http://dx.doi.org/10.1155/2014/438352 |
| spellingShingle | Ana O. Pires Andreia Neves-Carvalho Nuno Sousa António J. Salgado The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells Stem Cells International |
| title | The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells |
| title_full | The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells |
| title_fullStr | The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells |
| title_full_unstemmed | The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells |
| title_short | The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells |
| title_sort | secretome of bone marrow and wharton jelly derived mesenchymal stem cells induces differentiation and neurite outgrowth in sh sy5y cells |
| url | http://dx.doi.org/10.1155/2014/438352 |
| work_keys_str_mv | AT anaopires thesecretomeofbonemarrowandwhartonjellyderivedmesenchymalstemcellsinducesdifferentiationandneuriteoutgrowthinshsy5ycells AT andreianevescarvalho thesecretomeofbonemarrowandwhartonjellyderivedmesenchymalstemcellsinducesdifferentiationandneuriteoutgrowthinshsy5ycells AT nunosousa thesecretomeofbonemarrowandwhartonjellyderivedmesenchymalstemcellsinducesdifferentiationandneuriteoutgrowthinshsy5ycells AT antoniojsalgado thesecretomeofbonemarrowandwhartonjellyderivedmesenchymalstemcellsinducesdifferentiationandneuriteoutgrowthinshsy5ycells AT anaopires secretomeofbonemarrowandwhartonjellyderivedmesenchymalstemcellsinducesdifferentiationandneuriteoutgrowthinshsy5ycells AT andreianevescarvalho secretomeofbonemarrowandwhartonjellyderivedmesenchymalstemcellsinducesdifferentiationandneuriteoutgrowthinshsy5ycells AT nunosousa secretomeofbonemarrowandwhartonjellyderivedmesenchymalstemcellsinducesdifferentiationandneuriteoutgrowthinshsy5ycells AT antoniojsalgado secretomeofbonemarrowandwhartonjellyderivedmesenchymalstemcellsinducesdifferentiationandneuriteoutgrowthinshsy5ycells |