Decellularization techniques: unveiling the blueprint for tracheal tissue engineering
Certain congenital or acquired diseases and defects such as tracheo-oesophageal fistula, tracheomalacia, tracheal stenosis, airway ischemia, infections, and tumours can cause damage to the trachea. Treatments available do not offer any permanent solutions. Moreover, long-segment defects in the trach...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-02-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1518905/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850076761095667712 |
|---|---|
| author | Keisha T. Gomes Palla Ranga Prasad Jagnoor Singh Sandhu Jagnoor Singh Sandhu Ashwini Kumar Naveena A. N. Kumar N. B. Shridhar Bharti Bisht Manash K. Paul Manash K. Paul |
| author_facet | Keisha T. Gomes Palla Ranga Prasad Jagnoor Singh Sandhu Jagnoor Singh Sandhu Ashwini Kumar Naveena A. N. Kumar N. B. Shridhar Bharti Bisht Manash K. Paul Manash K. Paul |
| author_sort | Keisha T. Gomes |
| collection | DOAJ |
| description | Certain congenital or acquired diseases and defects such as tracheo-oesophageal fistula, tracheomalacia, tracheal stenosis, airway ischemia, infections, and tumours can cause damage to the trachea. Treatments available do not offer any permanent solutions. Moreover, long-segment defects in the trachea have no available surgical treatments. Tissue engineering has gained popularity in current regenerative medicine as a promising approach to bridge this gap. Among the various tissue engineering techniques, decellularization is a widely used approach that removes the cellular and nuclear contents from the tissue while preserving the native extracellular matrix components. The decellularized scaffolds exhibit significantly lower immunogenicity and retain the essential biomechanical and proangiogenic properties of native tissue, creating a foundation for trachea regeneration. The present review provides an overview of trachea decellularization advancements, exploring how recellularization approaches can be optimized by using various stem cells and tissue-specific cells to restore the scaffold’s structure and function. We examine critical factors such as mechanical properties, revascularization, and immunogenicity involved in the transplantation of tissue-engineered grafts. |
| format | Article |
| id | doaj-art-c892bcea5678496597887d3bb06501be |
| institution | DOAJ |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-c892bcea5678496597887d3bb06501be2025-08-20T02:45:56ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-02-011310.3389/fbioe.2025.15189051518905Decellularization techniques: unveiling the blueprint for tracheal tissue engineeringKeisha T. Gomes0Palla Ranga Prasad1Jagnoor Singh Sandhu2Jagnoor Singh Sandhu3Ashwini Kumar4Naveena A. N. Kumar5N. B. Shridhar6Bharti Bisht7Manash K. Paul8Manash K. Paul9Department of Radiation Biology and Toxicology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, IndiaDepartment of Radiation Biology and Toxicology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, IndiaCentral Animal Research Facility, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, IndiaCenter for Animal Research, Ethics and Training (CARET), Manipal Academy of Higher Education, Manipal, Karnataka, IndiaDepartment of Forensic Medicine, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, IndiaDepartment of Surgical Oncology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, IndiaDepartment of Pharmacology and Toxicology, Obscure Disease Research Center, Veterinary College Campus, Shivamogga, Karnataka, IndiaDepartment of Microbiology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, IndiaDepartment of Radiation Biology and Toxicology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, IndiaDivision of Pulmonary and Critical Care Medicine, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA, United StatesCertain congenital or acquired diseases and defects such as tracheo-oesophageal fistula, tracheomalacia, tracheal stenosis, airway ischemia, infections, and tumours can cause damage to the trachea. Treatments available do not offer any permanent solutions. Moreover, long-segment defects in the trachea have no available surgical treatments. Tissue engineering has gained popularity in current regenerative medicine as a promising approach to bridge this gap. Among the various tissue engineering techniques, decellularization is a widely used approach that removes the cellular and nuclear contents from the tissue while preserving the native extracellular matrix components. The decellularized scaffolds exhibit significantly lower immunogenicity and retain the essential biomechanical and proangiogenic properties of native tissue, creating a foundation for trachea regeneration. The present review provides an overview of trachea decellularization advancements, exploring how recellularization approaches can be optimized by using various stem cells and tissue-specific cells to restore the scaffold’s structure and function. We examine critical factors such as mechanical properties, revascularization, and immunogenicity involved in the transplantation of tissue-engineered grafts.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1518905/fulltracheadecellularizationrecellularizationscaffoldstransplantation |
| spellingShingle | Keisha T. Gomes Palla Ranga Prasad Jagnoor Singh Sandhu Jagnoor Singh Sandhu Ashwini Kumar Naveena A. N. Kumar N. B. Shridhar Bharti Bisht Manash K. Paul Manash K. Paul Decellularization techniques: unveiling the blueprint for tracheal tissue engineering Frontiers in Bioengineering and Biotechnology trachea decellularization recellularization scaffolds transplantation |
| title | Decellularization techniques: unveiling the blueprint for tracheal tissue engineering |
| title_full | Decellularization techniques: unveiling the blueprint for tracheal tissue engineering |
| title_fullStr | Decellularization techniques: unveiling the blueprint for tracheal tissue engineering |
| title_full_unstemmed | Decellularization techniques: unveiling the blueprint for tracheal tissue engineering |
| title_short | Decellularization techniques: unveiling the blueprint for tracheal tissue engineering |
| title_sort | decellularization techniques unveiling the blueprint for tracheal tissue engineering |
| topic | trachea decellularization recellularization scaffolds transplantation |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1518905/full |
| work_keys_str_mv | AT keishatgomes decellularizationtechniquesunveilingtheblueprintfortrachealtissueengineering AT pallarangaprasad decellularizationtechniquesunveilingtheblueprintfortrachealtissueengineering AT jagnoorsinghsandhu decellularizationtechniquesunveilingtheblueprintfortrachealtissueengineering AT jagnoorsinghsandhu decellularizationtechniquesunveilingtheblueprintfortrachealtissueengineering AT ashwinikumar decellularizationtechniquesunveilingtheblueprintfortrachealtissueengineering AT naveenaankumar decellularizationtechniquesunveilingtheblueprintfortrachealtissueengineering AT nbshridhar decellularizationtechniquesunveilingtheblueprintfortrachealtissueengineering AT bhartibisht decellularizationtechniquesunveilingtheblueprintfortrachealtissueengineering AT manashkpaul decellularizationtechniquesunveilingtheblueprintfortrachealtissueengineering AT manashkpaul decellularizationtechniquesunveilingtheblueprintfortrachealtissueengineering |