Endothelial cell proliferation and extracellular matrix-related pathways regulate pulmonary arterial hypertension in rat models
Objective To identify mechanisms regulating disease progression in rat models of pulmonary arterial hypertension (PAH). Methods Rat PAH models were established using subcutaneous monocrotaline (MCT) injection and the SU5416/hypoxia (SU/Hx) method. Transcriptomic sequencing of lung tissues was perfor...
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2025-06-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-6-770.pdf |
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| author | LU Xiaoxuan, GUO Lina, ZHANG Hong, SONG Wanlu, HU Yufei, YANG Peiran |
| author_facet | LU Xiaoxuan, GUO Lina, ZHANG Hong, SONG Wanlu, HU Yufei, YANG Peiran |
| author_sort | LU Xiaoxuan, GUO Lina, ZHANG Hong, SONG Wanlu, HU Yufei, YANG Peiran |
| collection | DOAJ |
| description | Objective To identify mechanisms regulating disease progression in rat models of pulmonary arterial hypertension (PAH). Methods Rat PAH models were established using subcutaneous monocrotaline (MCT) injection and the SU5416/hypoxia (SU/Hx) method. Transcriptomic sequencing of lung tissues was performed to identify gene expression and pathway alterations in PAH rats, followed by a comparative analysis with transcriptomic data of patients with idiopathic pulmonary arterial hypertension (IPAH) in NCBI database. Results Inflammatory-related genes such as CXCL9, CCL24, and SECTM1 were upregulated in both PAH rat models and IPAH patient lungs, while genes such as DGKG and DOCK9 were downregulated(P＜0.05). Pathways related to endothelial cell proliferation regulation and extracellular matrix (ECM) remodeling were significantly upregulated(P＜0.05). Conclusions The imbalance in endothelial cell proliferation and abnormal ECM remodeling may collectively contribute to PAH pathogenesis. Further exploration of these signaling pathways may provide deep insights for early diagnosis and targeted therapy of PAH. |
| format | Article |
| id | doaj-art-c88bdf853f7e4e2a88c4f4e0440fc957 |
| institution | Kabale University |
| issn | 1001-6325 |
| language | zho |
| publishDate | 2025-06-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj-art-c88bdf853f7e4e2a88c4f4e0440fc9572025-08-20T03:28:26ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252025-06-0145677077610.16352/j.issn.1001-6325.2025.06.0770Endothelial cell proliferation and extracellular matrix-related pathways regulate pulmonary arterial hypertension in rat modelsLU Xiaoxuan, GUO Lina, ZHANG Hong, SONG Wanlu, HU Yufei, YANG Peiran0Department of Physiology, Institute of Basic Medicine CAMS, School of Basic Medicine PUMC, Beijing 100005, ChinaObjective To identify mechanisms regulating disease progression in rat models of pulmonary arterial hypertension (PAH). Methods Rat PAH models were established using subcutaneous monocrotaline (MCT) injection and the SU5416/hypoxia (SU/Hx) method. Transcriptomic sequencing of lung tissues was performed to identify gene expression and pathway alterations in PAH rats, followed by a comparative analysis with transcriptomic data of patients with idiopathic pulmonary arterial hypertension (IPAH) in NCBI database. Results Inflammatory-related genes such as CXCL9, CCL24, and SECTM1 were upregulated in both PAH rat models and IPAH patient lungs, while genes such as DGKG and DOCK9 were downregulated(P＜0.05). Pathways related to endothelial cell proliferation regulation and extracellular matrix (ECM) remodeling were significantly upregulated(P＜0.05). Conclusions The imbalance in endothelial cell proliferation and abnormal ECM remodeling may collectively contribute to PAH pathogenesis. Further exploration of these signaling pathways may provide deep insights for early diagnosis and targeted therapy of PAH.https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-6-770.pdfpulmonary artery hypertension (pah)|animal model|endothelial cell|extracellular matrix (ecm) |
| spellingShingle | LU Xiaoxuan, GUO Lina, ZHANG Hong, SONG Wanlu, HU Yufei, YANG Peiran Endothelial cell proliferation and extracellular matrix-related pathways regulate pulmonary arterial hypertension in rat models Jichu yixue yu linchuang pulmonary artery hypertension (pah)|animal model|endothelial cell|extracellular matrix (ecm) |
| title | Endothelial cell proliferation and extracellular matrix-related pathways regulate pulmonary arterial hypertension in rat models |
| title_full | Endothelial cell proliferation and extracellular matrix-related pathways regulate pulmonary arterial hypertension in rat models |
| title_fullStr | Endothelial cell proliferation and extracellular matrix-related pathways regulate pulmonary arterial hypertension in rat models |
| title_full_unstemmed | Endothelial cell proliferation and extracellular matrix-related pathways regulate pulmonary arterial hypertension in rat models |
| title_short | Endothelial cell proliferation and extracellular matrix-related pathways regulate pulmonary arterial hypertension in rat models |
| title_sort | endothelial cell proliferation and extracellular matrix related pathways regulate pulmonary arterial hypertension in rat models |
| topic | pulmonary artery hypertension (pah)|animal model|endothelial cell|extracellular matrix (ecm) |
| url | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-6-770.pdf |
| work_keys_str_mv | AT luxiaoxuanguolinazhanghongsongwanluhuyufeiyangpeiran endothelialcellproliferationandextracellularmatrixrelatedpathwaysregulatepulmonaryarterialhypertensioninratmodels |