Viral inactivation in von Willebrand factor preparations via UVC irradiation: an experimental approach
[Objective] To evaluate the efficacy of ultraviolet-C (UVC) irradiation in inactivating porcine parvovirus (PPV), encephalomyocarditis virus (EMCV), pseudorabies virus (PRV) and vesicular stomatitis virus (VSV) within von Willebrand factor (vWF) preparations. [Methods] An ultraviolet inactivator (25...
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Institute of Blood Transfusion of Chinese Academy of Medical Sciences
2025-05-01
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| Series: | Zhongguo shuxue zazhi |
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| Online Access: | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.05.012&lang=en |
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| _version_ | 1849325284668473344 |
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| author | ZHANG Yalu MA Jie ZHANG Rong YANG Chunhui |
| author_facet | ZHANG Yalu MA Jie ZHANG Rong YANG Chunhui |
| author_sort | ZHANG Yalu |
| collection | DOAJ |
| description | [Objective] To evaluate the efficacy of ultraviolet-C (UVC) irradiation in inactivating porcine parvovirus (PPV), encephalomyocarditis virus (EMCV), pseudorabies virus (PRV) and vesicular stomatitis virus (VSV) within von Willebrand factor (vWF) preparations. [Methods] An ultraviolet inactivator (254 nm primary wavelength) was employed to optimize UV exposure dosages for the samples, and the initial test groups were set at five irradiation gradients: 100, 200, 400, 1 000, and 1 500 J/m2 gradients. Based on the results of the preliminary experiments, subsequent formal experiments implemented refined dosage parameters at 100, 125, 150, 175, and 200 J/m2. Virucidal efficacy against various pathogenic strains was evaluated via cytopathic effect (CPE) observation methodology, while simultaneously quantifying von Willebrand factor antigen levels (vWF∶Ag) and collagen-binding activity (vWF∶CBA) using enzyme-linked immunosorbent assays (ELISA) and automated coagulation analysis systems before and after UVC treatment. [Results] The results showed that > 100 J/m2 UVC irradiation achieved a≥4.0 log reduction factor in vWF. For vWF antigen, retention rates were observed to be 93.67%, 91.72%, 93.54%, 79.05%, and 85.50% at UVC doses of 100, 125, 150, 175, and 200 J/m2, respectively. Similarly, the retention rates of vWF collagen-binding activity were 81.11%, 87.81%, 86.15%, 73.73%, and 73.72% under the same dose conditions. [Conclusion] Considering both inactivation efficacy and functional preservation while ensuring safety and effectiveness, 100-150 J/m2 represents the optimal inactivation dose. |
| format | Article |
| id | doaj-art-c86f1a300aa64aed8b7c724a377faf13 |
| institution | Kabale University |
| issn | 1004-549X |
| language | zho |
| publishDate | 2025-05-01 |
| publisher | Institute of Blood Transfusion of Chinese Academy of Medical Sciences |
| record_format | Article |
| series | Zhongguo shuxue zazhi |
| spelling | doaj-art-c86f1a300aa64aed8b7c724a377faf132025-08-20T03:48:27ZzhoInstitute of Blood Transfusion of Chinese Academy of Medical SciencesZhongguo shuxue zazhi1004-549X2025-05-0138567367710.13303/j.cjbt.issn.1004-549x.2025.05.0121004-549X(2025)5-0673-05Viral inactivation in von Willebrand factor preparations via UVC irradiation: an experimental approachZHANG Yalu0MA Jie1ZHANG Rong2YANG Chunhui3Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, ChinaTaibang Biologic Group, Taian 271601, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, China[Objective] To evaluate the efficacy of ultraviolet-C (UVC) irradiation in inactivating porcine parvovirus (PPV), encephalomyocarditis virus (EMCV), pseudorabies virus (PRV) and vesicular stomatitis virus (VSV) within von Willebrand factor (vWF) preparations. [Methods] An ultraviolet inactivator (254 nm primary wavelength) was employed to optimize UV exposure dosages for the samples, and the initial test groups were set at five irradiation gradients: 100, 200, 400, 1 000, and 1 500 J/m2 gradients. Based on the results of the preliminary experiments, subsequent formal experiments implemented refined dosage parameters at 100, 125, 150, 175, and 200 J/m2. Virucidal efficacy against various pathogenic strains was evaluated via cytopathic effect (CPE) observation methodology, while simultaneously quantifying von Willebrand factor antigen levels (vWF∶Ag) and collagen-binding activity (vWF∶CBA) using enzyme-linked immunosorbent assays (ELISA) and automated coagulation analysis systems before and after UVC treatment. [Results] The results showed that > 100 J/m2 UVC irradiation achieved a≥4.0 log reduction factor in vWF. For vWF antigen, retention rates were observed to be 93.67%, 91.72%, 93.54%, 79.05%, and 85.50% at UVC doses of 100, 125, 150, 175, and 200 J/m2, respectively. Similarly, the retention rates of vWF collagen-binding activity were 81.11%, 87.81%, 86.15%, 73.73%, and 73.72% under the same dose conditions. [Conclusion] Considering both inactivation efficacy and functional preservation while ensuring safety and effectiveness, 100-150 J/m2 represents the optimal inactivation dose.https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.05.012&lang=enuvcvwfvirus inactivationppvprvemcvvsv |
| spellingShingle | ZHANG Yalu MA Jie ZHANG Rong YANG Chunhui Viral inactivation in von Willebrand factor preparations via UVC irradiation: an experimental approach Zhongguo shuxue zazhi uvc vwf virus inactivation ppv prv emcv vsv |
| title | Viral inactivation in von Willebrand factor preparations via UVC irradiation: an experimental approach |
| title_full | Viral inactivation in von Willebrand factor preparations via UVC irradiation: an experimental approach |
| title_fullStr | Viral inactivation in von Willebrand factor preparations via UVC irradiation: an experimental approach |
| title_full_unstemmed | Viral inactivation in von Willebrand factor preparations via UVC irradiation: an experimental approach |
| title_short | Viral inactivation in von Willebrand factor preparations via UVC irradiation: an experimental approach |
| title_sort | viral inactivation in von willebrand factor preparations via uvc irradiation an experimental approach |
| topic | uvc vwf virus inactivation ppv prv emcv vsv |
| url | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.05.012&lang=en |
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