<i>ε</i>-Poly-<i><span style="font-variant: small-caps">l</span></i>-lysine Suppressed Decay Development and Maintained Storage Quality in Guava Fruit by ROS Level Regulation and Antioxidant Ability Enhancement
Guava fruit is susceptible to decay, leading to losses in storability and quality. <i>ε</i>-Poly-<i><span style="font-variant: small-caps;">l</span></i>-lysine (<i>ε</i>-PL) is a safe antimicrobial polypeptide that has proven to be effective...
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MDPI AG
2025-03-01
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| author | Yingying An Li Li Mingming Wen Feng Luo Mei Tan Yuzhao Lin Hongbin Chen |
| author_facet | Yingying An Li Li Mingming Wen Feng Luo Mei Tan Yuzhao Lin Hongbin Chen |
| author_sort | Yingying An |
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| description | Guava fruit is susceptible to decay, leading to losses in storability and quality. <i>ε</i>-Poly-<i><span style="font-variant: small-caps;">l</span></i>-lysine (<i>ε</i>-PL) is a safe antimicrobial polypeptide that has proven to be effective in preserving produce’s quality. In the present research, <i>ε</i>-PL, at multiple concentrations (1, 2 and 4 g/L), was adopted to treat guavas, and the fruit were stored at 25 °C for 15 d. The results indicated that <i>ε</i>-PL retarded the guava storability decline and enhanced its quality. Treated guavas had a better appearance, as well as the lower disease index, relative electrolytic leakage, weight loss, respiration intensity, <i>a</i>* and <i>b</i>* values and reducing sugar content. They also showed higher firmness, commercially acceptable fruit rate, titratable acidity, <i>L</i>* value, total soluble sugar, vitamin C and sucrose levels. The optimal concentration of <i>ε</i>-PL was determined to be 2 g/L. Furthermore, compared to control guavas, fruit treated with 2 g/L <i>ε</i>-PL exhibited lower levels of superoxide anion, hydrogen peroxide and malondialdehyde but higher antioxidant enzyme activities in terms of ascorbate peroxidase, peroxidase, catalase and superoxide dismutase. These findings suggested that <i>ε</i>-PL raised the antioxidant enzyme activities to enhance the fruit’s antioxidant ability. This, in turn, reduced the reactive oxygen species levels and lipid peroxidation, ultimately improving the guava’s quality. Consequently, <i>ε</i>-PL is of practical significance for commercial application as it suppresses decay and stabilizes the quality of guavas, enhancing their postharvest marketability. |
| format | Article |
| id | doaj-art-c85fd0c2f2e94b4daeb7210ebc5e11b2 |
| institution | DOAJ |
| issn | 2077-0472 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Agriculture |
| spelling | doaj-art-c85fd0c2f2e94b4daeb7210ebc5e11b22025-08-20T02:41:54ZengMDPI AGAgriculture2077-04722025-03-0115665410.3390/agriculture15060654<i>ε</i>-Poly-<i><span style="font-variant: small-caps">l</span></i>-lysine Suppressed Decay Development and Maintained Storage Quality in Guava Fruit by ROS Level Regulation and Antioxidant Ability EnhancementYingying An0Li Li1Mingming Wen2Feng Luo3Mei Tan4Yuzhao Lin5Hongbin Chen6College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, ChinaCollege of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, ChinaCollege of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, ChinaCollege of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, ChinaCollege of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, ChinaCollege of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, ChinaCollege of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, ChinaGuava fruit is susceptible to decay, leading to losses in storability and quality. <i>ε</i>-Poly-<i><span style="font-variant: small-caps;">l</span></i>-lysine (<i>ε</i>-PL) is a safe antimicrobial polypeptide that has proven to be effective in preserving produce’s quality. In the present research, <i>ε</i>-PL, at multiple concentrations (1, 2 and 4 g/L), was adopted to treat guavas, and the fruit were stored at 25 °C for 15 d. The results indicated that <i>ε</i>-PL retarded the guava storability decline and enhanced its quality. Treated guavas had a better appearance, as well as the lower disease index, relative electrolytic leakage, weight loss, respiration intensity, <i>a</i>* and <i>b</i>* values and reducing sugar content. They also showed higher firmness, commercially acceptable fruit rate, titratable acidity, <i>L</i>* value, total soluble sugar, vitamin C and sucrose levels. The optimal concentration of <i>ε</i>-PL was determined to be 2 g/L. Furthermore, compared to control guavas, fruit treated with 2 g/L <i>ε</i>-PL exhibited lower levels of superoxide anion, hydrogen peroxide and malondialdehyde but higher antioxidant enzyme activities in terms of ascorbate peroxidase, peroxidase, catalase and superoxide dismutase. These findings suggested that <i>ε</i>-PL raised the antioxidant enzyme activities to enhance the fruit’s antioxidant ability. This, in turn, reduced the reactive oxygen species levels and lipid peroxidation, ultimately improving the guava’s quality. Consequently, <i>ε</i>-PL is of practical significance for commercial application as it suppresses decay and stabilizes the quality of guavas, enhancing their postharvest marketability.https://www.mdpi.com/2077-0472/15/6/654guava fruit<i>ε</i>-poly-<i><span style="font-variant: small-caps">l</span></i>-lysinestorabilitypostharvest qualityreactive oxygen speciesantioxidant enzymes |
| spellingShingle | Yingying An Li Li Mingming Wen Feng Luo Mei Tan Yuzhao Lin Hongbin Chen <i>ε</i>-Poly-<i><span style="font-variant: small-caps">l</span></i>-lysine Suppressed Decay Development and Maintained Storage Quality in Guava Fruit by ROS Level Regulation and Antioxidant Ability Enhancement Agriculture guava fruit <i>ε</i>-poly-<i><span style="font-variant: small-caps">l</span></i>-lysine storability postharvest quality reactive oxygen species antioxidant enzymes |
| title | <i>ε</i>-Poly-<i><span style="font-variant: small-caps">l</span></i>-lysine Suppressed Decay Development and Maintained Storage Quality in Guava Fruit by ROS Level Regulation and Antioxidant Ability Enhancement |
| title_full | <i>ε</i>-Poly-<i><span style="font-variant: small-caps">l</span></i>-lysine Suppressed Decay Development and Maintained Storage Quality in Guava Fruit by ROS Level Regulation and Antioxidant Ability Enhancement |
| title_fullStr | <i>ε</i>-Poly-<i><span style="font-variant: small-caps">l</span></i>-lysine Suppressed Decay Development and Maintained Storage Quality in Guava Fruit by ROS Level Regulation and Antioxidant Ability Enhancement |
| title_full_unstemmed | <i>ε</i>-Poly-<i><span style="font-variant: small-caps">l</span></i>-lysine Suppressed Decay Development and Maintained Storage Quality in Guava Fruit by ROS Level Regulation and Antioxidant Ability Enhancement |
| title_short | <i>ε</i>-Poly-<i><span style="font-variant: small-caps">l</span></i>-lysine Suppressed Decay Development and Maintained Storage Quality in Guava Fruit by ROS Level Regulation and Antioxidant Ability Enhancement |
| title_sort | i ε i poly i span style font variant small caps l span i lysine suppressed decay development and maintained storage quality in guava fruit by ros level regulation and antioxidant ability enhancement |
| topic | guava fruit <i>ε</i>-poly-<i><span style="font-variant: small-caps">l</span></i>-lysine storability postharvest quality reactive oxygen species antioxidant enzymes |
| url | https://www.mdpi.com/2077-0472/15/6/654 |
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